NCT05735717

Brief Summary

This is a phase II, open-label, prospective study of T cell receptor alpha/beta depletion (TCR α/β TCD) peripheral blood stem cell (PBSC) transplantation for children and adults with hematological malignancies. This is a safety/feasibility study of the investigational procedure/product.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
70

participants targeted

Target at P50-P75 for phase_2

Timeline
56mo left

Started May 2023

Longer than P75 for phase_2

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress40%
May 2023Nov 2030

First Submitted

Initial submission to the registry

January 10, 2023

Completed
1 month until next milestone

First Posted

Study publicly available on registry

February 21, 2023

Completed
3 months until next milestone

Study Start

First participant enrolled

May 11, 2023

Completed
4.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 30, 2027

Expected
3 years until next milestone

Study Completion

Last participant's last visit for all outcomes

November 30, 2030

Last Updated

July 24, 2025

Status Verified

July 1, 2025

Enrollment Period

4.6 years

First QC Date

January 10, 2023

Last Update Submit

July 21, 2025

Conditions

Keywords

BuFluG-CSFGFSRaGVHDHCTMACMelPBSCTPTLDRECISTTCR

Outcome Measures

Primary Outcomes (1)

  • Determine the rate of GVHD after alpha beta TCR depletion

    GVHD incidence after treatment.

    100 days

Secondary Outcomes (4)

  • Transplant engraftment

    42 days

  • Graft Failure

    100 days

  • Non-relapse mortality (NRM)

    12 months

  • Overall survival (OS)

    12 months

Study Arms (8)

Arm 1A: Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI Regimen, Closed to Accrual

EXPERIMENTAL

Patients will be treated on the most medically appropriate regimen with a preference for Flu/TBI Arm followed by an infusion at Day 0 of Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells.

Drug: FludarabineDrug: RituximabDrug: LevetiracetamBiological: Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells

Arm 2A: Fludarabine (flu), Busulfan (bu), Flu/Bu Regimen, Closed to Accrual

EXPERIMENTAL

Patients will be treated on the most medically appropriate regimen with a preference for Flu/TBI Arm followed by an infusion at Day 0 of Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells.

Drug: FludarabineDrug: BusulfanDrug: RituximabDrug: Levetiracetam

Arm 3A: Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients Only

EXPERIMENTAL

Flu/Bu/Mel will the preference for patients with JMML or infants with leukemia. , Closed to Accrual

Drug: FludarabineDrug: BusulfanDrug: MelphalanDrug: RituximabDrug: Levetiracetam

Arm 1B: ATG, Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI Regimen

EXPERIMENTAL

Patients will be treated on the most medically appropriate regimen with a preference for ATG/Flu/TBI Arm followed by an infusion at Day 0 of Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells.

Drug: FludarabineDrug: RituximabDrug: LevetiracetamDrug: Thymoglobulin

Arm 2B: ATG, Fludarabine (flu), Busulfan (bu), Flu/Bu Regimen

EXPERIMENTAL

Patients will be treated on the most medically appropriate regimen with a preference for ATG/Flu/BU/TBI Arm followed by an infusion at Day 0 of Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells.

Drug: FludarabineDrug: BusulfanDrug: RituximabDrug: LevetiracetamDrug: Thymoglobulin

Arm 3B: ATG, Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients Only

EXPERIMENTAL

ATG/Flu/Bu/Mel will the preference for patients with JMML or infants with leukemia.

Drug: FludarabineDrug: BusulfanDrug: MelphalanDrug: RituximabDrug: LevetiracetamDrug: Thymoglobulin

Arm 4B: ATG, Busulfan (BU), Cyclophosphamide (CY)

EXPERIMENTAL

Patients will be treated on the most medically appropriate regimen with a preference for ATG/BU/CY Arm followed by an infusion at Day 0 of Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells.

Drug: BusulfanDrug: RituximabDrug: LevetiracetamDrug: ThymoglobulinDrug: Cyclophosphamide

Arm 5B: ATG, Cyclophosphamide (CY), Total Body Irradiation (TBI)

EXPERIMENTAL

Patients will be treated on the most medically appropriate regimen with a preference for ATG/CY/TBI Arm followed by an infusion at Day 0 of Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells.

Drug: RituximabDrug: LevetiracetamDrug: ThymoglobulinDrug: Cyclophosphamide

Interventions

Fludarabine 25mg/m2 IV on days -8 to -6 or days -4 to -2. 40mg/m2 IV on days -5 to -2.

Arm 1A: Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI Regimen, Closed to AccrualArm 1B: ATG, Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI RegimenArm 2A: Fludarabine (flu), Busulfan (bu), Flu/Bu Regimen, Closed to AccrualArm 2B: ATG, Fludarabine (flu), Busulfan (bu), Flu/Bu RegimenArm 3A: Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 3B: ATG, Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients Only

Busulfan 82.1 mg\*hr/L IV on days -5 to -2 or days -8 to -5

Arm 2A: Fludarabine (flu), Busulfan (bu), Flu/Bu Regimen, Closed to AccrualArm 2B: ATG, Fludarabine (flu), Busulfan (bu), Flu/Bu RegimenArm 3A: Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 3B: ATG, Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 4B: ATG, Busulfan (BU), Cyclophosphamide (CY)

Melphalan 50 mg/m2 IV on days -4 to -2

Arm 3A: Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 3B: ATG, Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients Only

200 mg/m2 intravenous given once on day-1

Arm 1A: Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI Regimen, Closed to AccrualArm 1B: ATG, Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI RegimenArm 2A: Fludarabine (flu), Busulfan (bu), Flu/Bu Regimen, Closed to AccrualArm 2B: ATG, Fludarabine (flu), Busulfan (bu), Flu/Bu RegimenArm 3A: Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 3B: ATG, Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 4B: ATG, Busulfan (BU), Cyclophosphamide (CY)Arm 5B: ATG, Cyclophosphamide (CY), Total Body Irradiation (TBI)

As seizures have occurred following high dose busulfan, all patients will be treated with Keppra beginning day -6 and continuing until day -1 per institutional guidelines.

Also known as: Keppra
Arm 1A: Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI Regimen, Closed to AccrualArm 1B: ATG, Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI RegimenArm 2A: Fludarabine (flu), Busulfan (bu), Flu/Bu Regimen, Closed to AccrualArm 2B: ATG, Fludarabine (flu), Busulfan (bu), Flu/Bu RegimenArm 3A: Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 3B: ATG, Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 4B: ATG, Busulfan (BU), Cyclophosphamide (CY)Arm 5B: ATG, Cyclophosphamide (CY), Total Body Irradiation (TBI)

Patients will be treated on the most medically appropriate regimen followed by an infusion at Day 0 of Alpha/Beta T Cell-Depleted Hematopoietic Stem Cells.

Arm 1A: Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI Regimen, Closed to Accrual

rabbit anti-thymocyte globulin (rATG). Used in conditioning regimens for in vivo depletion of T cells, and the use of fludarabine model-based dosing to optimize dosing.

Also known as: ATG
Arm 1B: ATG, Fludarabine (flu), Total Body Irradiation (TBI), Flu/TBI RegimenArm 2B: ATG, Fludarabine (flu), Busulfan (bu), Flu/Bu RegimenArm 3B: ATG, Fludarabine (flu), Busulfan (bu), Melphalan (Mel) Regimen for Pediatric Patients OnlyArm 4B: ATG, Busulfan (BU), Cyclophosphamide (CY)Arm 5B: ATG, Cyclophosphamide (CY), Total Body Irradiation (TBI)

Cyclophosphamide 60 mg/kg IV over 2 hours on days -3 and -2

Arm 4B: ATG, Busulfan (BU), Cyclophosphamide (CY)Arm 5B: ATG, Cyclophosphamide (CY), Total Body Irradiation (TBI)

Eligibility Criteria

AgeUp to 60 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • Histological confirmation of hematological malignancies
  • Acute leukemias
  • Acute Myeloid Leukemia (AML) and related precursor neoplasms
  • Favorable risk AML is defined as having one of the following:
  • Acute lymphoblastic leukemia (ALL)/lymphoma
  • Myelodysplasia (MDS) IPSS INT-2 or High Risk (i.e. RAEB, RAEBt) or Refractory Anemia with severe pancytopenia, transfusion dependence, or high risk cytogenetics or molecular features.
  • Age 60 years of age or younger at the time of consent
  • Karnofsky performance status ≥ 70% or Lansky play score 50% for ≤16 years of age.
  • Adequate organ function

You may not qualify if:

  • Pregnant or breastfeeding.
  • Active uncontrolled infection within 1 week of starting preparative therapy
  • Known seropositive for HIV or known active Hepatitis B or C infection with detectable viral load by PCR.
  • Any prior autologous or allogeneic transplant
  • CML blast crisis
  • Active central nervous system malignancy

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Minnesota Masonic Cancer Center

Minneapolis, Minnesota, 55455, United States

RECRUITING

MeSH Terms

Conditions

Hematologic NeoplasmsLeukemia, Myeloid, AcutePrecursor Cell Lymphoblastic Leukemia-LymphomaChromosome AberrationsNeoplasm, ResidualAnemia, Refractory, with Excess of BlastsLeukemia, Myelomonocytic, JuvenileNeurofibromatosis 1Chromosome 7, monosomybeta-ThalassemiaInfluenza, Human

Interventions

fludarabineBusulfanMelphalanRituximabLevetiracetamthymoglobulinCyclophosphamide

Condition Hierarchy (Ancestors)

Neoplasms by SiteNeoplasmsHematologic DiseasesHemic and Lymphatic DiseasesLeukemia, MyeloidLeukemiaNeoplasms by Histologic TypeLeukemia, LymphoidLymphoproliferative DisordersLymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesPathologic ProcessesPathological Conditions, Signs and SymptomsNeoplastic ProcessesAnemia, RefractoryAnemiaMyelodysplastic SyndromesBone Marrow DiseasesMyelodysplastic-Myeloproliferative DiseasesNeurofibromatosesNeurofibromaNerve Sheath NeoplasmsNeoplasms, Nerve TissueNeoplastic Syndromes, HereditaryNeurocutaneous SyndromesNervous System DiseasesHeredodegenerative Disorders, Nervous SystemNeurodegenerative DiseasesPeripheral Nervous System DiseasesNeuromuscular DiseasesGenetic Diseases, InbornCongenital, Hereditary, and Neonatal Diseases and AbnormalitiesThalassemiaAnemia, Hemolytic, CongenitalAnemia, HemolyticHemoglobinopathiesRespiratory Tract InfectionsInfectionsOrthomyxoviridae InfectionsRNA Virus InfectionsVirus DiseasesRespiratory Tract Diseases

Intervention Hierarchy (Ancestors)

Butylene GlycolsGlycolsAlcoholsOrganic ChemicalsMesylatesAlkanesulfonatesAlkanesulfonic AcidsAlkanesHydrocarbons, AcyclicHydrocarbonsSulfonic AcidsSulfur AcidsSulfur CompoundsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedPhenylalanineAmino Acids, AromaticAmino Acids, CyclicAmino AcidsAmino Acids, Peptides, and ProteinsAntibodies, Monoclonal, Murine-DerivedAntibodies, MonoclonalAntibodiesImmunoglobulinsImmunoproteinsBlood ProteinsProteinsSerum GlobulinsGlobulinsAcetamidesAmidesAcetatesAcids, AcyclicCarboxylic AcidsPyrrolidinonesPyrrolidinesHeterocyclic Compounds, 1-RingHeterocyclic CompoundsPhosphoramide MustardsPhosphoramidesOrganophosphorus Compounds

Study Officials

  • Margaret MacMillan

    University of Minnesota Masonic Cancer Center

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Margaret MacMillan

CONTACT

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 10, 2023

First Posted

February 21, 2023

Study Start

May 11, 2023

Primary Completion (Estimated)

November 30, 2027

Study Completion (Estimated)

November 30, 2030

Last Updated

July 24, 2025

Record last verified: 2025-07

Data Sharing

IPD Sharing
Will not share

For the purposes of data and safety monitoring, this study is classified as high risk (investigator initiated under an IND). Therefore the following requirements will be fulfilled: * The Masonic Cancer Center Data and Safety Monitoring Council (DSMC) will review the study's progress at least quarterly. * The PI will comply with at least twice yearly monitoring of the project by the Masonic Cancer Center monitoring services. * The PI will oversee the submission of all reportable adverse events per the definition of reportable in Section 11.5 to the Masonic Cancer Center's SAE Coordinator, the University of Minnesota IRB, and the FDA.

Locations