NCT05627635

Brief Summary

This phase I/II trial tests the safety, side effects, best dose, and efficacy of FOLFOX and bevacizumab in combination with botensilimab and balstilimab (3B-FOLFOX) in treating patients with microsatellite stable (MSS) colorectal cancer that has spread from where it first started (primary site) to other places in the body (metastatic). Chemotherapy drugs, such as FOLFOX, work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing, or by stopping them from spreading. Bevacizumab is in a class of medications called antiangiogenic agents. It works by stopping the formation of blood vessels that bring oxygen and nutrients to tumor. This may slow the growth and spread of tumor. Balstilimab and botensilimab are in a class of medications called monoclonal antibodies. They bind to proteins, called PD-L1 and CTLA-4, which is found on some types of tumor cells. These PD-1 and CTLA-4 proteins are known to affect the body's defense mechanism to identify and fight against tumor cells. The combination of these drugs may lead to improved disease control and outcomes in patients with MSS metastatic colorectal cancer.

Trial Health

75
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
20

participants targeted

Target at P25-P50 for phase_1

Timeline
2mo left

Started May 2023

Typical duration for phase_1

Geographic Reach
1 country

1 active site

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress94%
May 2023Jun 2026

First Submitted

Initial submission to the registry

November 17, 2022

Completed
8 days until next milestone

First Posted

Study publicly available on registry

November 25, 2022

Completed
5 months until next milestone

Study Start

First participant enrolled

May 3, 2023

Completed
3.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 23, 2026

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 23, 2026

Last Updated

November 10, 2025

Status Verified

November 1, 2025

Enrollment Period

3.1 years

First QC Date

November 17, 2022

Last Update Submit

November 6, 2025

Conditions

Outcome Measures

Primary Outcomes (2)

  • Incidence of adverse events (phase I)

    Toxicity, graded according to the National Cancer Institute (NCI)-Common Terminology Criteria for Adverse Events (CTCAE) version (v)5.0. A dose limiting toxicity is a toxicity that occurs in the first 6 weeks of treatment with 3B-FOLFOX and that is attributed at least as possibly related to the study drugs

    Up to 6 weeks

  • Overall response (phase II)

    As assessed by Response Evaluation Criteria in Solid Tumors (RECIST) version (v)1.1. Will examine the proportion of the liver metastasis among the 60 enrolled patients and carry out subgroup analysis for overall response for patients with and without liver metastasis.

    Up to 5 years

Secondary Outcomes (8)

  • Overall response (phase I)

    Up to 5 years

  • Progression-free survival (PFS) (phase I)

    Time to disease progression/ relapse or death as a result of any cause, assessed up to 5 years

  • Overall survival (OS) (phase I)

    Time to death as a result of any cause, assessed up to 5 years

  • PFS (phase II)

    Time to disease progression or death as a result of any cause, assessed up to 5 years

  • OS (phase II)

    Time to death as a result of any cause, assessed up to 5 years

  • +3 more secondary outcomes

Study Arms (3)

Phase I (3B-FOLFOX)

EXPERIMENTAL

Patients receive FOLFOX, bevacizumab, balstilimab, and botensilimab IV on study. Patients undergo an x-ray, CT scan, PET scan, and/or MRI throughout the trial. Patients also undergo blood sample collection during screening and on study.

Biological: BalstilimabBiological: BevacizumabProcedure: Biospecimen CollectionBiological: BotensilimabProcedure: Computed TomographyDrug: FluorouracilDrug: Leucovorin CalciumProcedure: Magnetic Resonance ImagingDrug: OxaliplatinProcedure: Positron Emission TomographyProcedure: X-Ray Imaging

Phase II, Arm I (3B-FOLFOX)

EXPERIMENTAL

Patients receive FOLFOX, bevacizumab and balstilimab IV with botensilimab IV at a lower dose on study. Patients undergo an x-ray, CT scan, PET scan, and/or MRI throughout the trial. Patients also undergo blood sample collection during screening and on study.

Biological: BalstilimabBiological: BevacizumabProcedure: Biospecimen CollectionBiological: BotensilimabProcedure: Computed TomographyDrug: FluorouracilDrug: Leucovorin CalciumProcedure: Magnetic Resonance ImagingDrug: OxaliplatinProcedure: Positron Emission TomographyProcedure: X-Ray Imaging

Phase II, Arm II (3B-FOLFOX)

EXPERIMENTAL

Patients receive FOLFOX, bevacizumab and balstilimab IV with botensilimab IV at a higher dose on study. Patients undergo an x-ray, CT scan, PET scan, and/or MRI throughout the trial. Patients also undergo blood sample collection during screening and on study.

Biological: BalstilimabBiological: BevacizumabProcedure: Biospecimen CollectionBiological: BotensilimabProcedure: Computed TomographyDrug: FluorouracilDrug: Leucovorin CalciumProcedure: Magnetic Resonance ImagingDrug: OxaliplatinProcedure: Positron Emission TomographyProcedure: X-Ray Imaging

Interventions

BalstilimabBIOLOGICAL

Given IV

Also known as: AGEN 2034, AGEN-2034, AGEN2034
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)
BevacizumabBIOLOGICAL

Given IV

Also known as: ABP 215, Anti-VEGF, Anti-VEGF Humanized Monoclonal Antibody, Anti-VEGF Monoclonal Antibody SIBP04, Anti-VEGF rhuMAb, Avastin, Bevacizumab awwb, Bevacizumab Biosimilar ABP 215, Bevacizumab Biosimilar BEVZ92, Bevacizumab Biosimilar BI 695502, Bevacizumab Biosimilar CBT 124, Bevacizumab Biosimilar CT-P16, Bevacizumab Biosimilar FKB238, Bevacizumab Biosimilar GB-222, Bevacizumab Biosimilar HD204, Bevacizumab Biosimilar HLX04, Bevacizumab Biosimilar IBI305, Bevacizumab Biosimilar LY01008, Bevacizumab Biosimilar MIL60, Bevacizumab Biosimilar Mvasi, Bevacizumab Biosimilar MYL-1402O, Bevacizumab Biosimilar QL 1101, Bevacizumab Biosimilar RPH-001, Bevacizumab Biosimilar SCT501, Bevacizumab Biosimilar Zirabev, Bevacizumab-awwb, Bevacizumab-bvzr, BP102, BP102 Biosimilar, HD204, Immunoglobulin G1 (Human-Mouse Monoclonal rhuMab-VEGF Gamma-Chain Anti-Human Vascular Endothelial Growth Factor), Disulfide With Human-Mouse Monoclonal rhuMab-VEGF Light Chain, Dimer, Mvasi, MYL-1402O, Recombinant Humanized Anti-VEGF Monoclonal Antibody, rhuMab-VEGF, SCT501, SIBP 04, SIBP-04, SIBP04, Zirabev
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Undergo a blood sample collection

Also known as: Biological Sample Collection, Biospecimen Collected, Specimen Collection
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)
BotensilimabBIOLOGICAL

Given IV

Also known as: AGEN 1181, AGEN-1181, AGEN1181, Anti-CTLA-4 Monoclonal Antibody AGEN1181
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Undergo a CT scan

Also known as: CAT, CAT Scan, Computed Axial Tomography, Computerized Axial Tomography, Computerized Tomography, CT, CT Scan, tomography
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Given IV

Also known as: 5 Fluorouracil, 5 Fluorouracilum, 5 FU, 5-Fluoro-2,4(1H, 3H)-pyrimidinedione, 5-Fluorouracil, 5-Fluracil, 5-Fu, 5FU, AccuSite, Carac, Fluoro Uracil, Fluouracil, Flurablastin, Fluracedyl, Fluracil, Fluril, Fluroblastin, Ribofluor, Ro 2-9757, Ro-2-9757
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Given IV

Also known as: Adinepar, Calcifolin, Calcium (6S)-Folinate, Calcium Folinate, Calcium Leucovorin, Calfolex, Calinat, Cehafolin, Citofolin, Citrec, Citrovorum Factor, Cromatonbic Folinico, Dalisol, Disintox, Divical, Ecofol, Emovis, Factor, Citrovorum, Flynoken A, Folaren, Folaxin, FOLI-cell, Foliben, Folidan, Folidar, Folinac, Folinate Calcium, folinic acid, Folinic Acid Calcium Salt Pentahydrate, Folinoral, Folinvit, Foliplus, Folix, Imo, Lederfolat, Lederfolin, Leucosar, leucovorin, Rescufolin, Rescuvolin, Tonofolin, Wellcovorin
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Undergo MRI

Also known as: Magnetic Resonance, Magnetic Resonance Imaging Scan, Medical Imaging, Magnetic Resonance / Nuclear Magnetic Resonance, MR, MR Imaging, MRI, MRI Scan, NMR Imaging, NMRI, Nuclear Magnetic Resonance Imaging
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Given IV

Also known as: 1-OHP, Ai Heng, Aiheng, Dacotin, Dacplat, Diaminocyclohexane Oxalatoplatinum, Eloxatin, Eloxatine, JM-83, Oxalatoplatin, Oxalatoplatinum, RP 54780, RP-54780, SR-96669
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Undergo a PET scan

Also known as: Medical Imaging, Positron Emission Tomography, PET, PET Scan, Positron Emission Tomography Scan, Positron-Emission Tomography, proton magnetic resonance spectroscopic imaging, PT
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)
X-Ray ImagingPROCEDURE

Undergo an x-ray

Also known as: Conventional X-Ray, Diagnostic Radiology, Medical Imaging, X-Ray, Radiographic Imaging, Radiography, RG, Static X-Ray, X-Ray
Phase I (3B-FOLFOX)Phase II, Arm I (3B-FOLFOX)Phase II, Arm II (3B-FOLFOX)

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Documented informed consent of the participant and/or legally authorized representative
  • Assent, when appropriate, will be obtained per institutional guidelines
  • Age: \>= 18 years
  • Eastern Cooperative Oncology Group (ECOG) =\< 1
  • Life expectancy \>= 3 months
  • Patients should have a pathologically proven diagnosis of colorectal adenocarcinoma
  • Histological or cytological confirmed microsatellite stable (MSS) adenocarcinoma of colon or rectum. Microsatellite status should be performed per local standard of practice (immunohistochemistry \[IHC\] and or polymerase chain reaction \[PCR\], or next-generation sequencing, the presence of POLE mutations will be collected if available through next-generation sequencing)
  • Patients should have measurable metastatic disease as per Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 guidelines
  • Patients should not have a history of perforations or fistulas
  • For the safety cohorts (phase I): Metastatic colorectal cancer with 0 to 2 prior lines of therapy prior to enrollment on study and without prior progression within 3 months from last dose of oxaliplatin, in the event of prior oxaliplatin exposure. Evidence of radiographic progression after last treatment before enrollment should be documented
  • Patients with prior FOLFOX therapy should not have required dose modifications and should not have experienced unacceptable toxicities
  • Patients with other prior 5-FU-based therapies should not have required prior fluorouracil (5-FU) dose modifications below 2400 mg/m\^2 every 2 weeks
  • No prior oxaliplatin hypersensitivity
  • weeks should have elapsed from last prior chemotherapy and initiation of study treatment
  • For the efficacy cohorts (phase II): No prior treatment for metastatic disease. If prior FOLFOX adjuvant therapy was administered, there should be no evidence of disease relapse within the first 12 months after completion of adjuvant therapy
  • +16 more criteria

You may not qualify if:

  • Prior immunotherapy
  • Patients with a condition requiring systemic treatment with either corticosteroids (\> 10 mg daily prednisone equivalent) within 14 days or another immunosuppressive medication within 30 days of the first dose of study treatment. Inhaled or topical steroids, and adrenal replacement steroid doses \> 10 mg daily prednisone equivalent, are permitted in the absence of active autoimmune disease
  • Prior allogeneic organ transplantation
  • Surgical intervention within 4 weeks prior to study treatment, except for minor procedures such as port placement
  • Prior allergic reaction or hypersensitivity to any of the study drug components
  • Active autoimmune disease or history of autoimmune disease that required systemic treatment within 2 years before starting treatment, i.e., with use of disease-modifying agents or immunosuppressive drugs
  • Uncontrolled hypertension, defined as systolic blood pressure (SBP) \>150, diastolic blood pressure (DBP) \> 90
  • History of acute thrombotic venous events in the last 30 days before enrollment. If within 30 days, the patient should be on anticoagulants and without symptoms
  • Clinically significant (i.e., active) cardiovascular disease: cerebral vascular accident/stroke or myocardial infarction within 12 months of enrollment, unstable angina, congestive heart failure (New York Heart Association class \>= III), or serious uncontrolled cardiac arrhythmia requiring medication
  • Obstructive bowel symptoms related to unresected primary or carcinomatosis
  • Any persistent toxicities (Common Terminology Criteria for Adverse Events \[CTCAE\] grade \>= 2) from prior cancer therapy, excluding endocrinopathies stable on medication, stable neuropathy that is grade 1 or less, and alopecia
  • Non-healing wounds
  • Symptomatic active bleeding
  • Grade \>= 2 proteinuria as demonstrated by \>= 2+ protein and \>= 1.0 g of protein with 24-hour urine collection (patients found to have \>= 2+ protein on dipstick urinalysis must have 24-hour urine collection and demonstrate \< 1 g of protein in 24 hours in order to be eligible for the study)
  • Active brain metastases or leptomeningeal metastases with the following exceptions:
  • +13 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

City of Hope Medical Center

Duarte, California, 91010, United States

Location

MeSH Terms

Conditions

Colonic NeoplasmsRectal NeoplasmsColorectal Neoplasms

Interventions

balstilimabBevacizumabImmunoglobulin GDisulfidesSpecimen HandlingFluorouracildehydroftorafurLeucovorinMagnetic Resonance SpectroscopyOxaliplatinX-RaysPhantoms, Imaging

Condition Hierarchy (Ancestors)

Intestinal NeoplasmsGastrointestinal NeoplasmsDigestive System NeoplasmsNeoplasms by SiteNeoplasmsDigestive System DiseasesGastrointestinal DiseasesColonic DiseasesIntestinal DiseasesRectal Diseases

Intervention Hierarchy (Ancestors)

Antibodies, Monoclonal, HumanizedAntibodies, MonoclonalAntibodiesImmunoglobulinsImmunoproteinsBlood ProteinsProteinsAmino Acids, Peptides, and ProteinsSerum GlobulinsGlobulinsImmunoglobulin IsotypesSulfidesAnionsIonsElectrolytesInorganic ChemicalsHydrogen SulfideSulfur CompoundsOrganic ChemicalsClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisInvestigative TechniquesUracilPyrimidinonesPyrimidinesHeterocyclic Compounds, 1-RingHeterocyclic CompoundsFormyltetrahydrofolatesTetrahydrofolatesFolic AcidPterinsPteridinesHeterocyclic Compounds, 2-RingHeterocyclic Compounds, Fused-RingCoenzymesEnzymes and CoenzymesSpectrum AnalysisChemistry Techniques, AnalyticalCoordination ComplexesElectromagnetic RadiationElectromagnetic PhenomenaMagnetic PhenomenaPhysical PhenomenaRadiationRadiation, IonizingEquipment and Supplies

Study Officials

  • Marwan G Fakih

    City of Hope Medical Center

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
SEQUENTIAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 17, 2022

First Posted

November 25, 2022

Study Start

May 3, 2023

Primary Completion (Estimated)

June 23, 2026

Study Completion (Estimated)

June 23, 2026

Last Updated

November 10, 2025

Record last verified: 2025-11

Locations