NCT04370301

Brief Summary

This initial cohort of this phase II trial studied the outcomes of using a JAK inhibitor prior to reduced intensity haploidentical (Haplo) transplantation for the treatment of primary or secondary myelofibrosis (MF). The primary risk of using Haplo HCT in patients with MF is graft failure. In the first cohort, all patients engrafted. There were no instances of graft failure. However, a large number of patients did have graft versus host disease as a complication of their transplant. JAK inhibitors have since been approved for the indication of graft versus host disease treatment. And we are also using them for graft versus host disease prevention in a study of MF patients with sibling and unrelated donors. Therefore, we are opening a new cohort of the current study using the JAK inhibitor prior to, during and after Haplo transplant. Our goal is to decrease graft versus host disease in patients receiving a Haplo MF transplant without increasing the risk of graft failure.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
20

participants targeted

Target at below P25 for phase_2

Timeline
40mo left

Started Feb 2021

Longer than P75 for phase_2

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress61%
Feb 2021Aug 2029

First Submitted

Initial submission to the registry

April 28, 2020

Completed
2 days until next milestone

First Posted

Study publicly available on registry

April 30, 2020

Completed
10 months until next milestone

Study Start

First participant enrolled

February 9, 2021

Completed
6.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 30, 2027

Expected
2.3 years until next milestone

Study Completion

Last participant's last visit for all outcomes

August 31, 2029

Last Updated

January 12, 2026

Status Verified

January 1, 2026

Enrollment Period

6.2 years

First QC Date

April 28, 2020

Last Update Submit

January 8, 2026

Conditions

Outcome Measures

Primary Outcomes (1)

  • Probability of primary and secondary graft failure

    Primary graft failure is defined as failure to achieve an absolute neutrophil count of \> 500/ul by 42 days after bone marrow or peripheral blood stem cell transplantation. Secondary graft failure is defined as cytopenias after initial engraftment, with (a) donor chimerism of \< 5% or (b) falling donor chimerism with intervention such as second transplant or donor lymphocyte infusion or (c) patient death due to cytopenias, and fall in donor chimerism, even if chimerism is \> 5%. Exclusion criteria for diagnosis of graft failure are (a) disease relapse, (b) graft-versus-host disease, and (c) other causes of cytopenias such as, viral infection and drug toxicity.

    Up to 5 years

Secondary Outcomes (9)

  • Incidence of severe (grade 3 or 4) cytokine release syndrome

    Up to 5 years

  • Non-relapse mortality (NRM)

    Day 100

  • NRM

    1 year

  • Overall survival

    1 year

  • Overall survival

    3 years

  • +4 more secondary outcomes

Study Arms (2)

Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)

EXPERIMENTAL

JAK INHIBITOR THERAPY: Patients receive a JAK inhibitor at least 8 weeks prior to the start of HCT conditioning through day -4 before transplantation. CONDITIONING: Patients receive melphalan IV over 1 hour on day -5, fludarabine IV over 30-60 minutes on days -5 to -2, and undergo TBI on day -1 or day -1 and day 0. TRANSPLANT: Patients receive peripheral blood stem cell infusion on day 0. GVHD PROPHYLAXIS: Patients then receive cyclophosphamide IV over 3 hours on days 3-4, tacrolimus IV beginning day 5 then PO for about 6 months, mycophenolate mofetil PO BID or TID beginning day 5 for 6 weeks, and G-CSF SC beginning day 7 until neutrophil recovery is \> 1,500/mm\^3. All patients undergo MRI, CT, bone marrow biopsy and aspiration and blood sample collection throughout the trial. Patients also undergo ECHO or MUGA on the trial.

Drug: CyclophosphamideDrug: JAK InhibitorDrug: FludarabineBiological: Recombinant Granulocyte Colony-Stimulating FactorDrug: MelphalanDrug: Mycophenolate MofetilProcedure: Peripheral Blood Stem Cell TransplantationDrug: TacrolimusRadiation: Total-Body IrradiationProcedure: Computed TomographyProcedure: Magnetic Resonance ImagingProcedure: Bone Marrow BiopsyProcedure: Bone Marrow AspirationProcedure: Biospecimen CollectionProcedure: Echocardiography TestProcedure: Multigated Acquisition Scan

Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

EXPERIMENTAL

JAK INHIBITOR THERAPY: Patients receive a JAK inhibitor at least 8 weeks prior to the start of HCT conditioning through day -4 before transplantation. Additionally, patients receive a JAK inhibitor following transplantation beginning day 5 through 9-12 months after transplant. CONDITIONING: Patients receive melphalan IV over 1 hour on day -5, fludarabine IV over 30-60 minutes on days -5 to -2, and undergo TBI on day -1 or day -1 and day 0. TRANSPLANT: Patients receive peripheral blood stem cell infusion on day 0. GVHD PROPHYLAXIS: Patients then receive cyclophosphamide IV over 3 hours on days 3-4, tacrolimus IV beginning day 5 then PO for about 6 months, mycophenolate mofetil PO BID or TID beginning day 5 for 6 weeks, and G-CSF SC beginning day 7 until neutrophil recovery is \> 1,500/mm\^3. All patients undergo MRI, CT, bone marrow biopsy and aspiration and blood sample collection throughout the trial. Patients also undergo ECHO or MUGA on the trial.

Drug: CyclophosphamideDrug: JAK InhibitorDrug: FludarabineBiological: Recombinant Granulocyte Colony-Stimulating FactorDrug: MelphalanDrug: Mycophenolate MofetilProcedure: Peripheral Blood Stem Cell TransplantationDrug: TacrolimusRadiation: Total-Body IrradiationProcedure: Computed TomographyProcedure: Magnetic Resonance ImagingProcedure: Bone Marrow BiopsyProcedure: Bone Marrow AspirationProcedure: Biospecimen CollectionProcedure: Echocardiography TestProcedure: Multigated Acquisition Scan

Interventions

Given SC

Also known as: Recombinant Colony-Stimulating Factor 3, rhG-CSF, 143011-72-7
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Given IV

Also known as: PBPC transplantation, PBSCT, Peripheral Blood Progenitor Cell Transplantation, peripheral stem cell support, Peripheral Stem Cell Transplant, peripheral stem cell transplantation
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo TBI

Also known as: TBI, Total Body Irradiation, Whole Body Irradiation, Whole-Body Irradiation, Total-Body Irradiation Prior to Stem Cell Transplant, SCT_TBI
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo CT

Also known as: CAT Scan, Computed Axial Tomography, CT SCAN
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo MRI

Also known as: MR Imaging, MRI
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo bone marrow biopsy and aspiration

Also known as: Biopsy of Bone Marrow
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo bone marrow biopsy and aspiration

Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo ECHO

Also known as: EC
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo MUGA

Also known as: Blood Pool Scan, Equilibrium Radionuclide Angiography, Gated Blood Pool Imaging, Gated Heart Pool Scan, MUGA, MUGA Scan, Multi-Gated Acquisition Scan, Radionuclide Ventriculogram Scan, RNVG, SYMA Scanning, Synchronized Multigated Acquisition Scanning
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Given IV

Also known as: (-)-Cyclophosphamide, 2H-1,3,2-Oxazaphosphorine, 2-[bis(2-chloroethyl)amino]tetrahydro-, 2-oxide, monohydrate, Carloxan, Ciclofosfamida, Ciclofosfamide, Cicloxal, Clafen, Claphene, CP monohydrate, CTX, CYCLO-cell, Cycloblastin, Cycloblastine, Cyclophospham, Cyclophosphamid monohydrate, Cyclophosphamide Monohydrate, Cyclophosphamidum, Cyclophosphan, Cyclophosphane, Cyclophosphanum, Cyclostin, Cyclostine, Cytophosphan, Cytophosphane, Cytoxan, Fosfaseron, Genoxal, Genuxal, Ledoxina, Mitoxan, Neosar, Revimmune, Syklofosfamid, WR- 138719, Asta B 518, B-518, WR-138719
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Given PO

Also known as: JAK inhibitors, Janus Kinase Inhibitor, Ruxolitinib, Fedratinib
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Given IV

Also known as: Fluradosa
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Given IV

Also known as: Alanine Nitrogen Mustard, CB-3025, L-PAM, L-Phenylalanine mustard, L-sarcolysin, L-Sarcolysin Phenylalanine mustard, L-Sarcolysine, Melphalanum, Phenylalanine Mustard, Phenylalanine Nitrogen Mustard, Sarcoclorin, Sarkolysin, WR-19813
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Given PO

Also known as: Cellcept, MMF
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Given IV and PO

Also known as: FK 506, Fujimycin, Hecoria, Prograf, Protopic, FK-506, Tacforius
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Undergo blood sample collection

Also known as: Biological Sample Collection
Cohort I (JAK inhibitor, conditioning, GVHD prophylaxis)Cohort II (JAK inhibitor, conditioning, GVHD prophylaxis)

Eligibility Criteria

Age18 Years - 70 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Age between 18 and 70 years
  • Diagnosis of primary myelofibrosis (PMF) as defined by the 2016 World Health Organization classification system or diagnosis of secondary MF as defined by the International Working Group (IWG) for Myeloproliferative Neoplasms Research and Treatment criteria
  • Patients meeting the criteria for intermediate-1, intermediate-2 or high-risk disease by the Dynamic International Prognostic Scoring System (DIPSS)-plus scoring system (DIPSS may be used if all data from DIPSS are not available)
  • Ability to understand and the willingness to sign a written informed consent document (or legally authorized representative)
  • Patient must be a potential hematopoietic stem cell transplant candidate
  • Meeting criteria for 1st phase as above, at time of initiation of JAK inhibitor, including ability to understand and willingness to sign a written informed consent (or legally authorized representative). Patients arriving to our institution for transplant and not enrolled in Part 1 may still be enrolled in Part 2 if Part 1 criteria met. These patients will have Part 1 endpoints transcribed from medical records
  • Received JAK inhibitor for at least 8 weeks immediately prior to conditioning and be willing to continue until 9-12 months post-transplant as tolerated
  • Karnofsky performance status score \>= 70
  • Calculated creatinine clearance using the Cockcroft-Gault formula or 24 hour (hr) urine creatinine clearance must be \> 60 ml/min
  • Total serum bilirubin must be \< 3 mg/dL unless the elevation is thought to be due to Gilbert's disease or hemolysis
  • Transaminases must be \< 3 x the upper limit of normal
  • Patients with clinical or laboratory evidence of liver disease will be evaluated for the cause of liver disease, its clinical severity in terms of liver function, and the degree of portal hypertension. Patients with fulminant liver failure, cirrhosis with evidence of portal hypertension or bridging fibrosis, alcoholic hepatitis, hepatic encephalopathy, or correctable hepatic synthetic dysfunction evidenced by prolongation of the prothrombin time, ascites related to portal hypertension, bacterial or fungal abscess, biliary obstruction, chronic viral hepatitis with total serum bilirubin \> 3 mg/dL, and symptomatic biliary disease will be excluded
  • Diffusion capacity of the lung for carbon monoxide (DLCO) corrected \> 60% normal; may not be on supplemental oxygen
  • Left ventricular ejection fraction \> 40% OR shortening fraction \> 26%
  • Comorbidity Index \< 5 at the time of pre-transplant evaluation
  • +4 more criteria

You may not qualify if:

  • Contraindication to receiving a JAK inhibitor including:
  • Patients who have known hypersensitivity to JAK inhibitors
  • Clinical or laboratory evidence of significant renal or hepatic impairment including cirrhosis
  • Active uncontrolled infection
  • Known human immunodeficiency virus (HIV) positivity
  • Women who are pregnant or trying to conceive
  • Caution should be used in patients with platelets \< 100 though adjustments in dose can be made to accommodate anyone with platelets \> 50
  • History of prior allogeneic transplant
  • Leukemic transformation (\> 20% blasts)
  • Uncontrolled viral or bacterial infection at the time of study enrollment
  • Active or recent (prior 6 month) invasive fungal infection without infectious disease (ID) consult and approval
  • Known HIV positivity
  • Pregnant or breastfeeding
  • Availability of an human leukocyte antigen (HLA)-identical or 1-allele-mismatched related donor or an HLA 10 of 10 matched unrelated donor

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Fred Hutch/University of Washington Cancer Consortium

Seattle, Washington, 98109, United States

RECRUITING

MeSH Terms

Conditions

Primary Myelofibrosis

Interventions

CyclophosphamideJanus Kinase Inhibitorsruxolitinibfedratinibfludarabinepegylated granulocyte colony-stimulating factorMelphalanMycophenolic AcidPeripheral Blood Stem Cell TransplantationTacrolimusWhole-Body IrradiationMagnetic Resonance Spectroscopy

Condition Hierarchy (Ancestors)

Myeloproliferative DisordersBone Marrow DiseasesHematologic DiseasesHemic and Lymphatic Diseases

Intervention Hierarchy (Ancestors)

Phosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus CompoundsProtein Kinase InhibitorsEnzyme InhibitorsMolecular Mechanisms of Pharmacological ActionPharmacologic ActionsChemical Actions and UsesPhenylalanineAmino Acids, AromaticAmino Acids, CyclicAmino AcidsAmino Acids, Peptides, and ProteinsCaproatesAcids, AcyclicCarboxylic AcidsFatty AcidsLipidsHematopoietic Stem Cell TransplantationStem Cell TransplantationCell TransplantationCell- and Tissue-Based TherapyBiological TherapyTherapeuticsTransplantationSurgical Procedures, OperativeMacrolidesLactonesRadiotherapyInvestigative TechniquesSpectrum AnalysisChemistry Techniques, Analytical

Study Officials

  • Rachel B. Salit

    Fred Hutch/University of Washington Cancer Consortium

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Rachel B. Salit

CONTACT

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

April 28, 2020

First Posted

April 30, 2020

Study Start

February 9, 2021

Primary Completion (Estimated)

April 30, 2027

Study Completion (Estimated)

August 31, 2029

Last Updated

January 12, 2026

Record last verified: 2026-01

Data Sharing

IPD Sharing
Will not share

Locations