Effect of Non-essential Amino Acids on Protein Requirements for Endurance Athletes

NCT02874638 | Completed

• 1 other identifier: IAAO-EA3
• Study Type: interventional
• Target: 7
• Locations: 0 countries
• Sites: N/A

Brief Summary

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA). The biological value of protein depends on its amino acid composition. Essential amino acids are believed to have main role on whole body protein metabolism. However, it remains to be determined what role non-essential amino acids (NEAA) may have in regulating protein metabolism and contributing to the increased protein requirements after endurance exercise. The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. However, the IAAO method has never been utilized for assessing the effect of NEAA on protein requirements after endurance exercise. The objective of the current study is to investigate the importance of NEAA for endurance-trained male subjects.

Conditions

Increased Metabolic Requirement; Healthy

Outcome Measures

Primary Outcomes (1)

• 13CO2 (carbon dioxide) excretion rate (μmol/kg/h)

  8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.

  at 8 hours after the end of exercise]

Secondary Outcomes (1)

• [13C]phenylalanine oxidation rate (μmol/kg/h)

  at 8 hours after the end of exercise

Study Arms (4)

BASE egg protein

EXPERIMENTAL

0.8 g/kg/d of BASE protein provided as crystalline amino acid made after egg protein.

Dietary Supplement: BASE egg protein

BCAA-enriched egg protein

EXPERIMENTAL

branched-chain amino acid-enriched egg protein

Dietary Supplement: BCAA-enriched egg protein

small amount of essential amino acids

EXPERIMENTAL

small amount of essential amino acids made after egg protein, which is equivalent to the amount of essential amino acids in BASE

Dietary Supplement: small amount of essential amino acids

large amount of essential amino acids

EXPERIMENTAL

large amount of essential amino acids made after egg protein, which is equivalent to the amount of amino acids in BCAA

Dietary Supplement: large amount of essential amino acids

Interventions

BASE egg protein DIETARY_SUPPLEMENT

0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein

BASE egg protein

BCAA-enriched egg protein DIETARY_SUPPLEMENT

BCAA-enriched egg protein provided as crystalline amino acid.

BCAA-enriched egg protein

small amount of essential amino acids DIETARY_SUPPLEMENT

essential amino acids modeled after egg protein, provided as crystalline amino acid, which is equivalent to the amount of essential amino acid in BASE egg protein

small amount of essential amino acids

large amount of essential amino acids DIETARY_SUPPLEMENT

essential amino acids modeled after egg protein, provided as crystalline amino acid, which is equivalent to the amount of amino acid in BCAA-enriched egg protein

large amount of essential amino acids

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• endurance-trained participants who regularly run more than 40 km/week
• Participants who are categorized at least "very good" based on a study by Shvartz \& Reibold, in which VO2peak is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y)
• Ability to complete the 16-km familiarization run in session 2

You may not qualify if:

• Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
• Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
• Regular tobacco use
• Illicit drug use

Sponsors & Collaborators

• University of Toronto: lead
• Ajinomoto Co., Inc.: collaborator

Study Officials

• Daniel Moore, Ph.D.

  University of Toronto

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: August 17, 2016
• First Posted: August 22, 2016
• Study Start: September 1, 2016
• Primary Completion: August 1, 2017
• Study Completion: December 31, 2017
• Last Updated: April 1, 2019

Record last verified: 2019-03

Data Sharing

• IPD Sharing: Will not share