Protein Requirements in Resistance Trained Females

NCT03397043 | Completed

• 1 other identifier: RTF
• Study Type: interventional
• Target: 8
• Locations: 0 countries
• Sites: N/A

Brief Summary

In this study, the investigators will use the minimally invasive indicator amino acid oxidation (IAAO) technique to determine protein requirements in weight-trained males. It is hypothesized that the present study will show that protein requirements for weight-trained females are i) greater than the current RDA for non-active individual's comparable estimates, and ii) greater than existing nitrogen balance-based estimates for weight-training individuals

Conditions

Healthy

Outcome Measures

Primary Outcomes (1)

• Phenylalanine oxidation

  In µmol/kg/h, the product of F13CO2 and VCO2. F13CO2 is determined via breath enrichment analysis of 13CO2 after 1-13C phenylalanine ingestion, and VCO2 is determined via indirect calorimetry

  Through study completion: an average of 6 metabolic trials (days), each separated by approximately 1 week

Secondary Outcomes (2)

• Phenylalanine rate of appearance

  Through study completion: an average of 6 metabolic trials (days), each separated by approximately 1 week

• Net protein balance

  Through study completion: an average of 6 metabolic trials (days), each separated by approximately 1 week

Study Arms (1)

Healthy females

EXPERIMENTAL

All participants receive the intervention: Protein intake (dose). This consists of varying levels of dietary protein intakes, in the form of crystalline amino acids, ranging from 0.2-3.0 g/kg/d

Other: Protein intake (dose)

Interventions

Protein intake (dose) OTHER

Protein intake, in the form of crystalline amino acids, will range between 0.2-3.0 g/kg/d

Healthy females

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy female weight-trained individuals that have trained consistently for \>1 year
• complete a physical activity readiness questionnaire (PAR-Q+)
• Train each muscle group (i.e. chest, back, legs) at least twice a week
• Meets strength relative to body weight GUIDELINES adapted from Morton et al., 2016; Chilibeck et al., 1997 (sex specific).
• Bench Press:
• body weight (kg)\*0.7
• Leg Press:
• body weight (kg)\*2.3

You may not qualify if:

• sedentary for greater than 1 month in the last 6 months prior to the study
• non-weight stable for the past month
• smoker
• Illicit drug use (e.g. growth hormone, testosterone, etc.)
• Individual plans to increase or decrease body mass in the next 3 months
• Habitually ingests greater than or equal to 3g protein kg/bw/day
• Use of supplements such as creatine and beta-alanine in the last 30 days.
• Inability to adhere to any of the protocol guidelines

Sponsors & Collaborators

• University of Toronto: lead

Study Officials

• Daniel R Moore, PhD

  University of Toronto

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Masking Details: Participants blinded to the protein dose consumed in a given metabolic trial
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: January 5, 2018
• First Posted: January 11, 2018
• Study Start: November 1, 2016
• Primary Completion: October 6, 2017
• Study Completion: November 30, 2018
• Last Updated: March 29, 2019

Record last verified: 2019-03