Rate-limiting Amino Acids in Endurance-trained Athlete

NCT02628249 | Completed

• 1 other identifier: IAAO-EA
• Study Type: interventional
• Target: 5
• Locations: 0 countries
• Sites: N/A

Brief Summary

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA). The biological value of protein depends on the amino acid composition. As liebig's law of the minimum, the protein synthesis is limited due to the lack of single rate limiting amino acid. In this case, the first rate limiting amino acid determines protein intakes to maximize the protein synthesis. Adding a small amount of rate-limiting amino acid to the diet improves the biological value of its protein diet. The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. however, the IAAO method has never been utilized for determining the first rate limiting amino acid. Therefore, the purpose of this study is to address of IAAO method to be applied for determining the rate-limiting amino acid in endurance athlete.

Conditions

Increased Metabolic Requirement; Healthy

Outcome Measures

Primary Outcomes (1)

• 13CO2 (carbon dioxide) excretion rate (μmol/kg/h)

  8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.

  At 8 hours after the end of exercise

Secondary Outcomes (1)

• [13C]phenylalanine oxidation rate (μmol/kg/h)

  at 8 hours after the end of exercise

Study Arms (5)

Base protein

EXPERIMENTAL

0.8 g/kg/d of protein provided as crystalline amino acid made after egg protein.

Dietary Supplement: Base protein

Sufficient protein

EXPERIMENTAL

1.75 g/kg/d protein provided as crystalline amino acid made after egg protein.

Dietary Supplement: Sufficient protein

Base + BCAA

EXPERIMENTAL

Base protein intake + Branched chain amino acids

Dietary Supplement: Base protein; Dietary Supplement: Branched chain amino acids

Base + EAA

EXPERIMENTAL

Base protein intake + essential amino acids.

Dietary Supplement: Base protein; Dietary Supplement: Essential amino acids

Base + NEAA

EXPERIMENTAL

Base protein intake + non essential amino acids

Dietary Supplement: Base protein; Dietary Supplement: Non essential amino acids

Interventions

Base protein DIETARY_SUPPLEMENT

0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein

Base + BCAA; Base + EAA; Base + NEAA; Base protein

Sufficient protein DIETARY_SUPPLEMENT

1.75 g/kg/d protein provided as crystalline amino acid modeled after egg protein.

Sufficient protein

Branched chain amino acids DIETARY_SUPPLEMENT

Branched chain amino acids modeled after egg protein.

Base + BCAA

Essential amino acids DIETARY_SUPPLEMENT

Essential amino acids modeled after egg protein.

Base + EAA

Non essential amino acids DIETARY_SUPPLEMENT

Non essential amino acids modeled after egg protein.

Base + NEAA

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Endurance-trained participants who regularly more than 40 km/week
• Ability to perform the exercise stimulus (20 km run) on metabolic trial.

You may not qualify if:

• Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
• Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
• Regular tobacco use
• Illicit drug use

Sponsors & Collaborators

• University of Toronto: lead

Related Publications (1)

• Kato H, Suzuki K, Bannai M, Moore DR. Branched-Chain Amino Acids Are the Primary Limiting Amino Acids in the Diets of Endurance-Trained Men after a Bout of Prolonged Exercise. J Nutr. 2018 Jun 1;148(6):925-931. doi: 10.1093/jn/nxy048.

  PMID: 29746639 DERIVED

MeSH Terms

Interventions

Amino Acids; Amino Acids, Essential

Intervention Hierarchy (Ancestors)

Amino Acids, Peptides, and Proteins

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant professaor

Study Record Dates

• First Submitted: December 7, 2015
• First Posted: December 11, 2015
• Study Start: September 1, 2015
• Primary Completion: June 1, 2017
• Study Completion: December 1, 2017
• Last Updated: April 1, 2019

Record last verified: 2019-03