The Impact of Protein Intake on Protein Metabolism During Intensified Training

NCT02801344 | Completed

• 1 other identifier: PFE
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA). Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake. The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.

Conditions

Healthy

Keywords

exercise; performance; recovery; muscle; protein

Outcome Measures

Primary Outcomes (1)

• Change in 24h whole body protein balance

  Protein balance (mg/kg/d; protein synthesis - protein breakdown) will be measured using \[15N\]Glycine method on day 1 and day 4 to determine the change over the intensified training period to compare between protein levels

  Difference between day 1 and day 4 whole body protein balance

Secondary Outcomes (3)

• Change from baseline physical performance (5 km time trial) 5days after the beginning of training

  5days after the beginning of training

• change from baseline Physical performance (muscle strength) 5days after the beginning of training

  5days after the beginning of training

• change from baseline Physical Performance (muscle power) 5days after the beginning of training

  5days after the beginning of training

Other Outcomes (5)

• Creatine Phosphate Kinase activity on day 5

  5days after the beginning of training

• Myoglobin concentration on day 5

  5days after the beginning of training

• IL-6 concentration on day 5

  5days after the beginning of training

Study Arms (3)

Normal protein intake

EXPERIMENTAL

participants will receive the controlled-diet containing 0.8 g protein /kg/day and the test drink containing 0.14 g protein/kg/d.

Dietary Supplement: Normal; Other: Controlled-diet

Moderate protein intake

EXPERIMENTAL

participants will receive the controlled-diet containing 1.20 g protein /kg/day and the test drink containing 0.40 g protein/kg/d.

Dietary Supplement: Moderate; Other: Controlled-diet

High protein intake

EXPERIMENTAL

participants will receive the controlled-diet containing 1.83 g protein /kg/day and the test drink containing 1.03 g protein/kg/d.

Dietary Supplement: High; Other: Controlled-diet

Interventions

Normal DIETARY_SUPPLEMENT

test drink which contains low amount of amino acids (0.14 g/kg/day)

Normal protein intake

Moderate DIETARY_SUPPLEMENT

test drink which contains moderate amount of amino acids (0.40 g/kg/day)

Moderate protein intake

High DIETARY_SUPPLEMENT

test drink which contains high amount of amino acids (1.03 g/kg/day)

High protein intake

Controlled-diet OTHER

the diet containing 0.8 g protein /kg/day

High protein intake; Moderate protein intake; Normal protein intake

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy, male, endurance-trained participants who have run regularly more than 45 km or 4.5 hours/week
• Participants who are categorized at least "very good" based on a study by Shvartz \& Reibold \[40\], in which peak rate of oxygen consumption(VO2 Peak) is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y), ≥47 ml/kg/min (40-44 y), ≥44 ml/kg/min (45-50 y) according to his age,
• Participants who can cover 10 km in less than 60 min after the VO2peak test and 5 km Time trial on session 2.
• Participants will be 18-50 years old.
• Participants are willing to abide by the compliance rules of this study

You may not qualify if:

• Inability to meet health and physical activity guidelines according to the The Physical Activity Readiness Questionnaire for everyone.
• Female
• Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor
• Regular tobacco use
• Illicit drug use (e.g. growth hormone, testosterone, etc.)

Sponsors & Collaborators

• University of Toronto: lead
• Ajinomoto Co., Inc.: collaborator

Study Sites (1)

Goldring Centre For High Performance Sport

Toronto, Ontario, M5S 2C9, Canada

Location

Related Publications (1)

• Williamson E, Kato H, Volterman KA, Suzuki K, Moore DR. Greater plasma essential amino acids and lower 3-methylhistidine with higher protein intake during endurance training: a randomised control trial. Amino Acids. 2023 Oct;55(10):1285-1291. doi: 10.1007/s00726-022-03210-z. Epub 2022 Dec 7.

  PMID: 36477889 DERIVED

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Daniel Moore, Ph.D.

  Faculty of Kinesiology and Physical Education, University of Toronto

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate professor

Study Record Dates

• First Submitted: May 25, 2016
• First Posted: June 15, 2016
• Study Start: May 1, 2016
• Primary Completion: September 1, 2017
• Study Completion: February 1, 2018
• Last Updated: March 29, 2019

Record last verified: 2019-03

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)