NCT07466316

Brief Summary

This phase III trial compares higher dose chemotherapy, with vincristine, dactinomycin and cyclophosphamide, over a shorter amount of time to lower dose chemotherapy plus maintenance, with vincristine, dactinomycin, cyclophosphamide, irinotecan and vinorelbine, over a longer amount of time, along with standard of care surgery and radiation, in patients with newly diagnosed intermediate risk rhabdomyosarcoma. Vincristine and vinorelbine are in a class of medications called vinca alkaloids. They work by stopping tumor cells from growing and dividing and may kill them. Dactinomycin is a type of antibiotic that is only used in cancer chemotherapy (antineoplastic antibiotic). It works by damaging the cell's DNA and may kill tumor cells. Cyclophosphamide is in a class of medications called alkylating agents. It works by damaging the cell's DNA and may kill tumor cells. It may also lower the body's immune response. Irinotecan is in a class of antineoplastic medications called topoisomerase I inhibitors. It blocks a certain enzyme needed for cell division and DNA repair and may kill tumor cells. It is not yet known if the higher dose chemotherapy over a shorter amount of time or the lower dose chemotherapy with maintenance over a longer amount of time is more effective in the treatment of patient with newly diagnosed, intermediate risk rhabdomyosarcoma.

Trial Health

65
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
342

participants targeted

Target at P50-P75 for phase_3

Timeline
58mo left

Started Jun 2026

Longer than P75 for phase_3

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

January 28, 2026

Completed
1 month until next milestone

First Posted

Study publicly available on registry

March 12, 2026

Completed
3 months until next milestone

Study Start

First participant enrolled

June 22, 2026

Expected
4.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 31, 2031

Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 31, 2031

Last Updated

March 12, 2026

Status Verified

March 1, 2026

Enrollment Period

4.8 years

First QC Date

January 28, 2026

Last Update Submit

March 11, 2026

Conditions

Outcome Measures

Primary Outcomes (1)

  • Event free survival (EFS)

    Will be estimated using the Kaplan-Meier method and will be compared between the randomized treatment groups using the stratified log-rank test.

    From randomization until the first occurrence of progression or relapse, second malignancy, or death, up to 5 years

Secondary Outcomes (5)

  • Overall survival (OS)

    From randomization to death from any cause, up to 5 years

  • Clinician-reported adverse events (AEs)

    Up to 5 years

  • Proportion of patients who successfully undergo real-time review of delayed primary excision (DPE)

    Up to week 12 of treatment

  • Local failure rate for patients deemed eligible for DPE

    Up to 5 years

  • Percentage of molecular biomarker testing that is resulted within the 6-week timeframe

    Up to cycle 3 (each cycle is 21 days)

Other Outcomes (16)

  • Somatic molecular featuresTP53 mutation

    Up to 5 years

  • Somatic molecular features MYCN amplification TP53 mutation

    Up to 5 years

  • Somatic molecular features MYOD1 mutation

    Up to 5 years

  • +13 more other outcomes

Study Arms (2)

Regimen A (Higher cyclophosphamide dose regimen

EXPERIMENTAL

See Detailed Description for Regimen A.

Procedure: Biospecimen CollectionProcedure: Bone Marrow AspirationProcedure: Bone Marrow BiopsyProcedure: Bone ScanProcedure: Computed TomographyDrug: CyclophosphamideBiological: DactinomycinProcedure: Lumbar PunctureProcedure: Lymph Node BiopsyProcedure: Magnetic Resonance ImagingProcedure: Positron Emission TomographyRadiation: Radiation TherapyProcedure: ResectionOther: Survey AdministrationDrug: Vincristine Sulfate

Regimen B (Lower cyclophosphamide dose, maintenance)

EXPERIMENTAL

See Detailed Description for Regimen B.

Procedure: Biospecimen CollectionProcedure: Bone Marrow AspirationProcedure: Bone Marrow BiopsyProcedure: Bone ScanProcedure: Computed TomographyDrug: CyclophosphamideBiological: DactinomycinDrug: Irinotecan HydrochlorideProcedure: Lumbar PunctureProcedure: Lymph Node BiopsyProcedure: Magnetic Resonance ImagingProcedure: Positron Emission TomographyRadiation: Radiation TherapyProcedure: ResectionOther: Survey AdministrationDrug: Vincristine SulfateDrug: Vinorelbine Tartrate

Interventions

Undergo bone marrow biopsy

Also known as: Biopsy of Bone Marrow, Biopsy, Bone Marrow
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)
Bone ScanPROCEDURE

Undergo bone scan

Also known as: Bone Scintigraphy
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Undergo CT scan

Also known as: CAT, CAT Scan, Computed Axial Tomography, Computerized Axial Tomography, Computerized axial tomography (procedure), Computerized Tomography, Computerized Tomography (CT) scan, CT, CT Scan, Diagnostic CAT Scan, Diagnostic CAT Scan Service Type, tomography
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Given IV and PO

Also known as: (-)-Cyclophosphamide, 2H-1,3,2-Oxazaphosphorine, 2-[bis(2-chloroethyl)amino]tetrahydro-, 2-oxide, monohydrate, Asta B 518, B 518, B-518, B518, Carloxan, Ciclofosfamida, Ciclofosfamide, Cicloxal, Clafen, Claphene, CP monohydrate, CTX, CYCLO-cell, Cycloblastin, Cycloblastine, Cyclophospham, Cyclophosphamid monohydrate, Cyclophosphamide Monohydrate, Cyclophosphamidum, Cyclophosphan, Cyclophosphane, Cyclophosphanum, Cyclostin, Cyclostine, Cytophosphan, Cytophosphane, Cytoxan, Fosfaseron, Genoxal, Genuxal, Ledoxina, Mitoxan, Neosar, Revimmune, Syklofosfamid, WR 138719, WR- 138719, WR-138719, WR138719
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)
DactinomycinBIOLOGICAL

Given IV

Also known as: Actinomycin A IV, Actinomycin C1, Actinomycin D, Actinomycin I1, Actinomycin IV, Actinomycin X 1, Actinomycin-[thr-val-pro-sar-meval], Cosmegen, DACT, Dactinomycine, Lyovac Cosmegen, Meractinomycin
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Given IV

Also known as: Campto, Camptosar, Camptothecin 11, Camptothecin-11, CPT 11, CPT-11, CPT11, Irinomedac, Irinotecan Hydrochloride Trihydrate, Irinotecan Monohydrochloride Trihydrate, U 101440E, U-101440E, U101440E
Regimen B (Lower cyclophosphamide dose, maintenance)

Undergo lumbar puncture

Also known as: LP, Spinal Tap
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Undergo lymph node biopsy

Also known as: Biopsy of Lymph Node
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Undergo MRI

Also known as: Magnetic Resonance, Magnetic Resonance Imaging (MRI), Magnetic resonance imaging (procedure), Magnetic Resonance Imaging Scan, Medical Imaging, Magnetic Resonance / Nuclear Magnetic Resonance, MR, MR Imaging, MRI, MRI Scan, MRIs, NMR Imaging, NMRI, Nuclear Magnetic Resonance Imaging, sMRI, Structural MRI
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Undergo FDG PET scan

Also known as: Medical Imaging, Positron Emission Tomography, PET, PET Scan, Positron emission tomography (procedure), Positron Emission Tomography Scan, Positron-Emission Tomography, PT
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Undergo radiation therapy

Also known as: Cancer Radiotherapy, Energy Type, ENERGY_TYPE, Irradiate, Irradiated, Irradiation, Radiation, Radiation Therapy, NOS, Radiotherapeutics, Radiotherapy, RT, Therapy, Radiation
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)
ResectionPROCEDURE

Undergo resection surgery

Also known as: Surgical Resection
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Ancillary studies

Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Given IV

Also known as: Kyocristine, Leurocristine Sulfate, Leurocristine, sulfate, Oncovin, Vincasar, Vincosid, Vincrex, Vincristine, sulfate
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Given IV

Also known as: Biovelbin, Eunades, KW 2307, KW-2307, KW2307, Navelbine, Navelbine Ditartrate, NVB, Vinorelbine Ditartrate
Regimen B (Lower cyclophosphamide dose, maintenance)

Undergo blood and cerebrospinal fluid sample collection

Also known as: Biological Sample Collection, Biospecimen Collected, Specimen Collection
Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Undergo bone marrow aspiration

Regimen A (Higher cyclophosphamide dose regimenRegimen B (Lower cyclophosphamide dose, maintenance)

Eligibility Criteria

AgeUp to 50 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • Patient must be ≤ 50 years of age at the time of enrollment
  • Patients with newly diagnosed soft tissue RMS of any subtype, except adult-type pleomorphic, based upon institutional histopathologic classification, are eligible to enroll on the study based upon FOXO1 fusion status, Stage, Intergroup Rhabdomyosarcoma Study (IRS) group, and age, as below. FOXO1 fusion status must be determined prior to enrollment. RMS types included under embryonal rhabdomyosarcoma (ERMS) include those which are reclassified in the 2020 World Health Organization (WHO) classification as ERMS (typical, dense and botryoid variants) and spindle cell/sclerosing RMS (encompassing the historical spindle cell ERMS variant and the newly recognized sclerosing RMS variant). Classification of alveolar Rhabdomyosarcoma (ARMS) in the 2020 WHO Classification is the same as in the International Classification of Rhabdomyosarcoma (ICR) and includes classic and solid variants.
  • FOXO1 fusion negative (FN)
  • Stage 2/3, Group III
  • Stage 4, Group IV, \< 10 years old
  • FOXO1 fusion positive (FP)
  • Stages 1-3, Groups I-III
  • Disease/staging imaging studies, if applicable, must be obtained within 21 days prior to enrollment and start of protocol therapy (repeat if necessary)
  • FOXO1 status results must be available to enroll. All patients will undergo institutional pathology review and institutional FOXO1 fusion determination regardless of histology prior to enrollment. FOXO1 status may confirmed by cytogenetic, fluorescence in situ hybridization (FISH), or next generation sequencing techniques. FOXO1 fusion results should be SUBMITTED as an upload to RAVE at study enrollment because this information is required for randomization.
  • Please note the following:
  • Institutional PAX3 versus (vs.) PAX7 determination is not required but should be submitted if available. Institutional FOXO1 testing may be performed at a contract or commercial lab as long as reports can be submitted and uploaded to RAVE. Additional molecular pathology reports, including the MCI report, are not required but should be submitted if available.
  • Patients who are \< 10 years old with distant metastatic disease (Stage 4) who have institutional molecular testing indicating fusion negative (FN) RMS but are later found to have fusion positive (FP) RMS by MCI or other testing will be considered to have metastatic FP disease and will go off study
  • Appropriate lymph node sampling based on primary site of disease is required
  • Patients must have a performance status of Lansky performance status score ≥ 50 for patients ≤ 16 years of age or Karnofsky performance status score ≥ 50 for patients \> 16 years of age
  • Peripheral absolute neutrophil count (ANC) ≥ 750/μL (All laboratory studies to determine eligibility must be performed within 7 days prior to enrollment unless otherwise indicated. Laboratory studies must be repeated prior to the start of protocol therapy if \> 7 days have elapsed from their most recent prior assessment. Laboratory tests need not be repeated if therapy starts within seven (7) days of their most recent prior assessment. If the result of a laboratory study that is repeated at any time post-enrollment and prior to the start of protocol therapy is outside the limits for eligibility, then the evaluation must be rechecked within 48 hours prior to initiating protocol therapy. The results of the recheck must be within the limits for eligibility to proceed. If the result of the recheck is outside the limits of eligibility, the patient may not receive protocol therapy and will be considered off protocol therapy.)
  • +23 more criteria

You may not qualify if:

  • Patients with evidence of uncontrolled infection are not eligible
  • Previous or concurrent cancer(s) that is/was being treated with chemotherapy and/or radiation.
  • Note: Surgical resection alone of previous or concurrent cancer(s) is allowed
  • Patients with central nervous system involvement of RMS as defined below:
  • Malignant cells detected in cerebrospinal fluid
  • Intra-parenchymal brain metastases separate and distinct from primary tumor (i.e., direct extension from parameningeal primary tumors is allowed)
  • Diffuse leptomeningeal disease
  • Patients with known Charcot-Marie-Tooth disease
  • Patients who have received any chemotherapy (excluding steroids) and/or radiation therapy for RMS prior to enrollment. Note: the following exception:
  • Patients requiring emergency radiation therapy for life-threatening complications of tumor burden due to RMS. These patients are eligible, provided they are consented to ARST2531 prior to administration of radiation.
  • Note: Patients who have received or are receiving chemotherapy or radiation for non-malignant conditions (eg, autoimmune diseases) are eligible. Patients must discontinue chemotherapy for non-malignant conditions prior to starting protocol therapy
  • Female patients who are pregnant since fetal toxicities and teratogenic effects have been noted for several of the study drugs. A pregnancy test is required for female patients of childbearing potential
  • Lactating females who plan to breastfeed their infants
  • Sexually active patients of reproductive potential who have not agreed to use an effective contraceptive method for the duration of their study participation

Contact the study team to confirm eligibility.

Sponsors & Collaborators

MeSH Terms

Conditions

Rhabdomyosarcoma

Interventions

Specimen HandlingBiopsyCyclophosphamideDactinomycinIrinotecanSpinal PunctureSentinel Lymph Node BiopsyMagnetic Resonance SpectroscopyRadiotherapyRadiationVincristineVinorelbine

Condition Hierarchy (Ancestors)

MyosarcomaNeoplasms, Muscle TissueNeoplasms, Connective and Soft TissueNeoplasms by Histologic TypeNeoplasmsSarcoma

Intervention Hierarchy (Ancestors)

Clinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisInvestigative TechniquesCytodiagnosisCytological TechniquesDiagnostic Techniques, SurgicalSurgical Procedures, OperativePhosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus CompoundsHeterocyclic Compounds, 3-RingHeterocyclic Compounds, Fused-RingHeterocyclic CompoundsPeptides, CyclicMacrocyclic CompoundsPolycyclic CompoundsPeptidesAmino Acids, Peptides, and ProteinsCamptothecinAlkaloidsDiagnostic Techniques, NeurologicalPuncturesTherapeuticsLymph Node ExcisionSpectrum AnalysisChemistry Techniques, AnalyticalPhysical PhenomenaVinca AlkaloidsSecologanin Tryptamine AlkaloidsIndole AlkaloidsIndolesHeterocyclic Compounds, 2-RingIndolizidinesIndolizines

Study Officials

  • Christine M Heske

    Children's Oncology Group

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 3
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
NETWORK
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 28, 2026

First Posted

March 12, 2026

Study Start (Estimated)

June 22, 2026

Primary Completion (Estimated)

March 31, 2031

Study Completion (Estimated)

March 31, 2031

Last Updated

March 12, 2026

Record last verified: 2026-03