NCT05835687

Brief Summary

Loc3CAR is a Phase I clinical trial evaluating the use of autologous B7-H3-CAR T cells for participants ≤ 21 years old with primary CNS neoplasms. B7-H3-CAR T cells will be locoregionally administered via a CNS reservoir catheter. Study participants will be divided into two cohorts: cohort A with B7-H3-positive relapsed/refractory non-brainstem primary CNS tumors, and cohort B with diffuse midline gliomas (DMG). Participants will receive four (4) B7-H3-CAR T cell infusions over a 4 week period. The purpose of this study is to find the maximum (highest) dose of B7-H3-CAR T cells that are safe to give patients with primary brain tumors. Primary objectives

  • To determine the safety, maximum tolerated dose (MTD) and recommended phase 2 dose (RP2D) for the locoregional delivery of autologous B7-H3-CAR T cells in patients ≤ 21 years of age with recurrent/refractory B7-H3+ primary CNS tumors (Cohort A) or DMG (Cohort B). Secondary objectives
  • To assess the efficacy, defined as sustained objective response, a partial response (PR) or complete response (CR) observed anytime on active treatment with B7-H3-CAR T cells in patients with relapsed/refractory B7-H3+ primary CNS tumors (Cohort A) or DMG (Cohort B).
  • To characterize and monitor neurologic toxicities in patients while on study (Cohort A and B).

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
48

participants targeted

Target at P50-P75 for phase_1

Timeline
22mo left

Started Apr 2023

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress63%
Apr 2023Mar 2028

First Submitted

Initial submission to the registry

March 23, 2023

Completed
1 month until next milestone

Study Start

First participant enrolled

April 27, 2023

Completed
1 day until next milestone

First Posted

Study publicly available on registry

April 28, 2023

Completed
4.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 1, 2028

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 1, 2028

Last Updated

May 5, 2026

Status Verified

May 1, 2026

Enrollment Period

4.8 years

First QC Date

March 23, 2023

Last Update Submit

May 1, 2026

Conditions

Outcome Measures

Primary Outcomes (1)

  • Maximum tolerated dose (MTD)

    To determine the maximum tolerated dose for the locoregional delivery of autologous B7-H3-CAR T cells in patients with recurrent/refractory B7-H3- positive primary CNS tumors (Cohort A) or diffuse midline glioma (DMG) (Cohort B).

    Four (4) weeks after the first B7-H3-CAR T-cell infusion or 7 days after the fourth B7-H3-CAR T cell infusion, whichever is longer

Secondary Outcomes (1)

  • Radiographic response

    Four (4) weeks post B7-H3-CAR T-cell infusion

Study Arms (2)

Arm A (relapsed/refractory CNS tumors)

EXPERIMENTAL

Patients with B7-H3-positive relapsed/refractory non-brainstem primary CNS tumors.

Drug: B7-H3-CAR T cells

Arm B (diffuse midline gliomas [DMG])

EXPERIMENTAL

Patients with DMG.

Drug: B7-H3-CAR T cells

Interventions

Autologous T cells transduced with a lentiviral vector expressing a B7-H3-CAR with a CD28z signaling domain and 41BB ligand (B7-H3-CAR T cells). Four (4) infusions of B7-H3-CAR T cells will be locoregionally administered via CNS reservoir catheter.

Arm A (relapsed/refractory CNS tumors)Arm B (diffuse midline gliomas [DMG])

Eligibility Criteria

AgeUp to 21 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • Age ≤ 21 years of age
  • Primary CNS tumor
  • For Cohort A, must have evidence of relapsed or refractory non-brainstem CNS tumor
  • For Cohort B, must meet one of the following criteria:
  • Adequate tumor tissue from primary tumor resection or biopsy for central pathology review (i.e., B7-H3 expression evaluation by immunohistochemistry \[IHC\] or H3K27M mutation if pontine lesion)
  • Has a diagnosis of diffuse midline glioma that harbors a mutation associated with this entity (e.g. H3K27M)
  • Has presumptive/suspected brainstem high-grade neoplasm with available imaging for central imaging review
  • Life expectancy of \> 12 weeks
  • Adult patient, parent or legal guardian can understand and is willing to sign a written informed consent document according to institutional guidelines

You may not qualify if:

  • \. Participant has other clinically significant medical disorders (e.g. serious infections or significant cardiac, pulmonary, hepatic, psychiatric, or other organ dysfunction) that could compromise their ability to tolerate protocol therapy or would interfere with study procedure.
  • Age ≤ 21 years of age
  • Primary CNS tumor with measurable or evaluable disease and meets criteria for either Cohort A or B:
  • Cohort A: relapsed/refractory non-brainstem CNS primary tumor AND tumor is B7-H3 positive
  • Cohort B: Diffuse midline glioma AND tumor is:
  • B7-H3 positive if non-pontine
  • OR H3K27-altered diffuse midline pontine glioma
  • OR radiographically-confirmed classic/typical DIPG
  • Estimated life expectancy of \>12 weeks
  • Karnofsky or Lansky performance score ≥50
  • Participant of childbearing/child-fathering potential agrees to use contraception
  • For females of childbearing age:
  • Not pregnant with negative serum pregnancy test
  • Not lactating with intent to breastfeed
  • Chemotherapy/biologic therapy must be discontinued ≥ 7 days prior to enrollment
  • +66 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

St. Jude Children's Research Hospital

Memphis, Tennessee, 38105, United States

RECRUITING

Related Links

MeSH Terms

Conditions

Central Nervous System NeoplasmsRhabdoid TumorEpendymomaGliomaGlioblastomaMedulloblastoma

Condition Hierarchy (Ancestors)

Nervous System NeoplasmsNeoplasms by SiteNeoplasmsNervous System DiseasesNeoplasms, Complex and MixedNeoplasms by Histologic TypeNeoplasms, NeuroepithelialNeuroectodermal TumorsNeoplasms, Germ Cell and EmbryonalNeoplasms, Glandular and EpithelialNeoplasms, Nerve TissueAstrocytomaNeuroectodermal Tumors, Primitive

Study Officials

  • Christopher DeRenzo, MD

    St. Jude Children's Research Hospital

    PRINCIPAL INVESTIGATOR
  • Kelsey Bertrand, MD, MSc

    St. Jude Children's Research Hospital

    PRINCIPAL INVESTIGATOR
  • Giedre Krenciute, PhD

    St. Jude Children's Research Hospital

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Tabatha E. Doyle, RN

CONTACT

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 23, 2023

First Posted

April 28, 2023

Study Start

April 27, 2023

Primary Completion (Estimated)

March 1, 2028

Study Completion (Estimated)

March 1, 2028

Last Updated

May 5, 2026

Record last verified: 2026-05

Data Sharing

IPD Sharing
Will share

Individual participant de-identified datasets containing the variables analyzed in the published article will be made available (related to the study primary or secondary objectives contained in the publication). Supporting documents such as the protocol, statistical analyses plan, and informed consent are available through the CTG website for the specific study. Data used to generate the published article will be made available at the time of article publication. Investigators who seek access to individual level de-identified data will contact the computing team in the Department of Biostatistics (ClinTrialDataRequest@stjude.org) who will respond to the data request.

Shared Documents
STUDY PROTOCOL, SAP, ICF
Time Frame
Data will be made available at the time of article publication.
Access Criteria
Data will be provided to researchers following a formal request with the following information: full name of requestor, affiliation, data set requested, and timing of when data is needed. As an informational point, the lead statistician and study principal investigator will be informed that primary results datasets have been requested.

Locations