NCT02650986

Brief Summary

This phase I/IIa trial studies the side effects and best dose of gene-modified T cells when given with or without decitabine, and to see how well they work in treating patients with malignancies expressing cancer-testis antigens 1 (NY-ESO-1) gene that have spread to other places in the body (advanced). A T cell is a type of immune cell that can recognize and kill abnormal cells of the body. Placing a modified gene for NY-ESO-1 into the patients' T cells in the laboratory and then giving them back to the patient may help the body build an immune response to kill tumor cells that express NY-ESO-1. Drugs used in chemotherapy, such as decitabine, work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing, or by stopping them from spreading. It is not yet known whether giving gene-modified T cells with or without decitabine works better in treating patients with malignancies expressing NY-ESO-1.

Trial Health

75
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
15

participants targeted

Target at below P25 for phase_1

Timeline
76mo left

Started Jul 2017

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress59%
Jul 2017Jul 2032

First Submitted

Initial submission to the registry

December 21, 2015

Completed
18 days until next milestone

First Posted

Study publicly available on registry

January 8, 2016

Completed
1.5 years until next milestone

Study Start

First participant enrolled

July 14, 2017

Completed
3.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 4, 2021

Completed
1.2 years until next milestone

Results Posted

Study results publicly available

May 31, 2022

Completed
10.1 years until next milestone

Study Completion

Last participant's last visit for all outcomes

July 14, 2032

Expected
Last Updated

June 17, 2025

Status Verified

June 1, 2025

Enrollment Period

3.6 years

First QC Date

December 21, 2015

Results QC Date

May 4, 2022

Last Update Submit

June 12, 2025

Conditions

Advanced Fallopian Tube CarcinomaAdvanced Malignant Solid NeoplasmAdvanced MelanomaAdvanced Ovarian CarcinomaAdvanced Primary Peritoneal CarcinomaAdvanced Synovial SarcomaClinical Stage III Cutaneous Melanoma AJCC v8Clinical Stage IV Cutaneous Melanoma AJCC v8Metastatic Fallopian Tube CarcinomaMetastatic MelanomaMetastatic Ovarian CarcinomaMetastatic Primary Peritoneal CarcinomaMetastatic Synovial SarcomaPathologic Stage III Cutaneous Melanoma AJCC v8Pathologic Stage IIIA Cutaneous Melanoma AJCC v8Pathologic Stage IIIB Cutaneous Melanoma AJCC v8Pathologic Stage IIIC Cutaneous Melanoma AJCC v8Pathologic Stage IIID Cutaneous Melanoma AJCC v8Pathologic Stage IV Cutaneous Melanoma AJCC v8Platinum-Resistant Fallopian Tube CarcinomaPlatinum-Resistant Ovarian CarcinomaPlatinum-Resistant Primary Peritoneal CarcinomaStage III Fallopian Tube Cancer AJCC v8Stage III Ovarian Cancer AJCC v8Stage III Primary Peritoneal Cancer AJCC v8Stage IIIA Fallopian Tube Cancer AJCC v8Stage IIIA Ovarian Cancer AJCC v8Stage IIIA Primary Peritoneal Cancer AJCC v8Stage IIIA1 Fallopian Tube Cancer AJCC v8Stage IIIA1 Ovarian Cancer AJCC v8Stage IIIA2 Fallopian Tube Cancer AJCC v8Stage IIIA2 Ovarian Cancer AJCC v8Stage IIIB Fallopian Tube Cancer AJCC v8Stage IIIB Ovarian Cancer AJCC v8Stage IIIB Primary Peritoneal Cancer AJCC v8Stage IIIC Fallopian Tube Cancer AJCC v8Stage IIIC Ovarian Cancer AJCC v8Stage IIIC Primary Peritoneal Cancer AJCC v8Stage IV Fallopian Tube Cancer AJCC v8Stage IV Ovarian Cancer AJCC v8Stage IV Primary Peritoneal Cancer AJCC v8Stage IVA Fallopian Tube Cancer AJCC v8Stage IVA Ovarian Cancer AJCC v8Stage IVA Primary Peritoneal Cancer AJCC v8Stage IVB Fallopian Tube Cancer AJCC v8Stage IVB Ovarian Cancer AJCC v8Stage IVB Primary Peritoneal Cancer AJCC v8Unresectable MelanomaUnresectable Ovarian CarcinomaUnresectable Synovial Sarcoma

Outcome Measures

Primary Outcomes (2)

  • Number of Participants With Dose Limiting Toxicities

    Will be assessed using Common Toxicity Criteria. Patients will be monitored by medical histories, physical examinations, ophthalmologic exams, and blood studies to detect potential toxicities from the treatment. the number of patients experiencing a DLT are reported.

    Up to 30 days

  • Number of Participants With Feasibility Concerns in Manufacturing of NY-ESO-1/ dnTGFbetaRII Engineered Cells

    Unfeasibility will be defined as 3 or more preparations not meeting the lot release criteria in each cohort. Sufficient numbers of circulating, lot released confirmed T cells will be measured prior to administration. The count of patients not meeting the preparation criteria are reported.

    1 month

Secondary Outcomes (3)

  • Expression of the NY-ESO-1 T Cell Receptor (TCR) Transgenic Protein in Peripheral Blood Mononuclear Cells (PBMC)

    Up to 15 years

  • Replication Competent Retrovirus (RCR)

    Up to 1 year

  • Tumor Response (Complete and Partial Response)

    Up to 90 days after TCR transgenic PBMC adoptive transfer

Study Arms (2)

Cohort I (cyclophosphamide, TCR/dnTGFbetaRII)

EXPERIMENTAL

Patients undergo leukapheresis on day -6 and receive cyclophosphamide IV over 2 hours on days -5 and -4. Patients then receive TGFbDNRII-transduced autologous tumor infiltrating lymphocytes IV over 15 minutes on day 0. Eligible patients who showed initial response/disease control, may receive a second TGFbDNRII-transduced autologous tumor infiltrating lymphocytes infusion at any time after progression is confirmed.

Drug: CyclophosphamideOther: Laboratory Biomarker AnalysisProcedure: LeukapheresisBiological: TGFbDNRII-transduced Autologous Tumor Infiltrating Lymphocytes

Cohort II (decitabine, cyclophosphamide, TCR/dnTGFbetaRII)

EXPERIMENTAL

Patients undergo leukapheresis on day -6 and receive decitabine IV over 1 hour on days -6 to -4 and cyclophosphamide IV over 2 hours on days -3 and -2. Patients then receive TGFbDNRII-transduced autologous tumor infiltrating lymphocytes IV over 15 minutes on day 0. Eligible patients who showed initial response/disease control, may receive a second TGFbDNRII-transduced autologous tumor infiltrating lymphocytes infusion at any time after progression is confirmed.

Drug: CyclophosphamideDrug: DecitabineOther: Laboratory Biomarker AnalysisProcedure: LeukapheresisBiological: TGFbDNRII-transduced Autologous Tumor Infiltrating Lymphocytes

Interventions

Given IV

Also known as: (-)-Cyclophosphamide, 2H-1,3,2-Oxazaphosphorine, 2-[bis(2-chloroethyl)amino]tetrahydro-, 2-oxide, monohydrate, Carloxan, Ciclofosfamida, Ciclofosfamide, Cicloxal, Clafen, Claphene, CP monohydrate, CTX, CYCLO-cell, Cycloblastin, Cycloblastine, Cyclophospham, Cyclophosphamid monohydrate, Cyclophosphamide Monohydrate, Cyclophosphamidum, Cyclophosphan, Cyclophosphane, Cyclophosphanum, Cyclostin, Cyclostine, Cytophosphan, Cytophosphane, Cytoxan, Fosfaseron, Genoxal, Genuxal, Ledoxina, Mitoxan, Neosar, Revimmune, Syklofosfamid, WR- 138719
Cohort I (cyclophosphamide, TCR/dnTGFbetaRII)Cohort II (decitabine, cyclophosphamide, TCR/dnTGFbetaRII)

Given IV

Also known as: 5-Aza-2''-deoxycytidine, Dacogen, Decitabine for Injection, Deoxyazacytidine, Dezocitidine
Cohort II (decitabine, cyclophosphamide, TCR/dnTGFbetaRII)

Correlative studies

Cohort I (cyclophosphamide, TCR/dnTGFbetaRII)Cohort II (decitabine, cyclophosphamide, TCR/dnTGFbetaRII)
LeukapheresisPROCEDURE

Undergo leukapheresis

Also known as: Leukocytopheresis, Therapeutic Leukopheresis
Cohort I (cyclophosphamide, TCR/dnTGFbetaRII)Cohort II (decitabine, cyclophosphamide, TCR/dnTGFbetaRII)

Given IV

Also known as: TGFbDNRII-transduced Autologous TILs
Cohort I (cyclophosphamide, TCR/dnTGFbetaRII)Cohort II (decitabine, cyclophosphamide, TCR/dnTGFbetaRII)

Eligibility Criteria

Age12 Years+
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Patients with solid tumors as described below:
  • Inoperable or metastatic (advanced) melanoma:
  • Has received, is intolerant, or refused a CTLA-4 inhibitor (ipilimumab) or a PD-1 inhibitor (nivolumab or pembrolizumab) as monotherapy and/or a combination of ipilimumab and nivolumab
  • Has received or is intolerant of a BRAF inhibitor or the combination of BRAF and MEK inhibitors for BRAFv600 mutant melanoma and a PD-1 inhibitor as monotherapy or in combination
  • Inoperable or metastatic (advanced) ovarian, primary peritoneal or fallopian tube carcinoma:
  • Has received platinum containing chemotherapy and has platinum refractory or resistant disease that has progressed on second line therapy
  • If platinum sensitive disease, should have received \>= 2 lines of chemotherapy
  • May have received PARP inhibitors, bevacizumab or other targeted VEGF inhibitor therapy
  • Inoperable or metastatic (advanced) synovial sarcoma:
  • Should have received and progressed on \>= two lines of systemic therapy
  • Subjects with other histologies:
  • Must have previously received two lines of systemic standard care (or effective salvage chemotherapy regimens) for metastatic disease, if known to be effective for that disease, and have been deemed either non-responders (progressive disease) or have recurred
  • For cohorts 1, 2 and 3 only: Patient's tumor must be positive by histological or molecular assay for NY-ESO-1, according to the screening algorithm; historical results may be used
  • For cohort 4, NY-ESO-1 results will be noted but NY-ESO-1 positivity is not required for eligibility
  • Human leukocyte antigen (HLA)-A\*0201 (HLA-A2.1) positivity by molecular subtyping (blood test or buccal swab, historical documentation acceptable)
  • +26 more criteria

You may not qualify if:

  • Previously known hypersensitivity to any of the agents used in this study
  • Currently receiving any other investigational agents. Note: Patients who have suffered \>grade 2 irAEs during previous checkpoint inhibitor therapy should be excluded.
  • Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements; electrocardiogram (EKG) will be done at screening; cardiac stress test will be done as clinically indicated, the specific test to be chosen at the discretion of the treating physician.
  • History of severe autoimmune disease requiring steroids or other immunosuppressive treatments
  • History of inflammatory bowel disease, celiac disease, or other chronic gastrointestinal conditions associated with diarrhea or bleeding, which in the investigator's opinion would place the patient at an increased risk for adverse effect or current acute colitis of any origin; treated cases with no active disease are eligible
  • Potential requirement for systemic corticosteroids or concurrent immunosuppressive drugs based on prior history or received systemic steroids within the last 4 weeks prior to enrollment (inhaled or topical steroids at standard doses or isolated use of steroids as premedication for medical procedures to minimize allergic reaction \[e.g. computed tomography (CT) scan dye\] are allowed)
  • Known active infection with human immunodeficiency virus (HIV), hepatitis B, hepatitis C or cytomegalovirus (CMV)
  • Dementia or significantly altered mental status that would prohibit the understanding or rendering of compliance with the requirements of this protocol even with caregiver support
  • Pregnancy or breast-feeding; female patients must be surgically sterile or be postmenopausal for two years, or must agree to use effective contraception during the period of treatment and 6 months after; all female patients with reproductive potential must have a negative pregnancy test (serum/urine) within 48 hours from starting the conditioning chemotherapy
  • Lack of availability of a patient for immunological and clinical follow-up assessment

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Roswell Park Cancer Institute

Buffalo, New York, 14263, United States

Location

MeSH Terms

Conditions

Fallopian Tube NeoplasmsMelanomaOvarian NeoplasmsSarcoma, Synovial

Interventions

CyclophosphamideDecitabineInjectionsLeukapheresis

Condition Hierarchy (Ancestors)

Genital Neoplasms, FemaleUrogenital NeoplasmsNeoplasms by SiteNeoplasmsFallopian Tube DiseasesAdnexal DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital DiseasesNeuroendocrine TumorsNeuroectodermal TumorsNeoplasms, Germ Cell and EmbryonalNeoplasms by Histologic TypeNeoplasms, Nerve TissueNevi and MelanomasSkin NeoplasmsSkin DiseasesSkin and Connective Tissue DiseasesEndocrine Gland NeoplasmsOvarian DiseasesEndocrine System DiseasesGonadal DisordersNeoplasms, Connective TissueNeoplasms, Connective and Soft TissueSarcoma

Intervention Hierarchy (Ancestors)

Phosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus CompoundsAzacitidineAza CompoundsCytidinePyrimidine NucleosidesPyrimidinesHeterocyclic Compounds, 1-RingHeterocyclic CompoundsNucleosidesNucleic Acids, Nucleotides, and NucleosidesRibonucleosidesDrug Administration RoutesDrug TherapyTherapeuticsCytapheresisBiological TherapyBlood Component RemovalLeukocyte Reduction ProceduresCell SeparationCytological TechniquesClinical Laboratory TechniquesInvestigative Techniques

Results Point of Contact

Title
Kris Attwood
Organization
Roswell Park Comprehensive Cancer Center

Study Officials

  • Philip McCarthy, MD

    Roswell Park Cancer Institute

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
Yes

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
SEQUENTIAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 21, 2015

First Posted

January 8, 2016

Study Start

July 14, 2017

Primary Completion

March 4, 2021

Study Completion (Estimated)

July 14, 2032

Last Updated

June 17, 2025

Results First Posted

May 31, 2022

Record last verified: 2025-06

Locations