Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men

NCT04596553 | Completed

• 1 other identifier: H20-00943
• Study Type: interventional
• Target: 17
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.

Conditions

Skeletal Muscle; Extracellular Matrix; Amino Acids Metabolism; Exercise; Dietary Supplements

Keywords

Muscle Collagen Turnover; Myofibrillar Protein Synthesis; Extracellular Matrix; Anabolism Signaling; Resistance Exercise; Skeletal Muscle; Bone Turnover

Outcome Measures

Primary Outcomes (8)

• Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis

  Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.

  0 - 4 hours post-exercise

• Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis

  Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.

  0 - 2 hours post-exercise

• Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis

  Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.

  2 - 4 hours post-exercise

• Fractional synthetic rate (change over time) Muscle Collagen Synthesis

  Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.

  0 - 4 hours post-exercise

• Fractional synthetic rate (change over time) Muscle Collagen Synthesis

  Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.

  0 - 2 hours post-exercise

• Fractional synthetic rate (change over time) Muscle Collagen Synthesis

  Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.

  2 - 4 hours post-exercise

• Pro-Collagen Type I N-Propeptide (PINP)

  Osteoblastic marker of bone collagen synthesis

  1 hour prior to exercise

• Pro-Collagen Type I N-Propeptide (PINP)

  Osteoblastic marker of bone collagen synthesis

  4 hours post exercise

Secondary Outcomes (5)

• Plasma Amino Acids Response to Supplementation

  0 - 4 hours post-exercise

• Muscle mechanistic target of rapamycin Pathway Activation

  0 - 4 hours post-exercise

• PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV)

  0 - 4 hours post-exercise

• Pro-Collagen Type III N-Propeptide (PIIINP)

  1 hour prior to exercise

• Pro-Collagen Type III N-Propeptide (PIIINP)

  4 hours post exercise

Study Arms (3)

Essential Amino Acid

ACTIVE COMPARATOR

Participants will consume 1 dose of: 15 g crystalline essential amino acid supplement (Pure Encapsulation Essential Aminos 180) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water

Dietary Supplement: Essential Amino Acid

Collagen Peptide

ACTIVE COMPARATOR

Participants will consume 1 dose of: 15g collagen peptide (Gelita TENDOFORTE) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water

Dietary Supplement: Collagen Peptide

Maltodextrin Placebo

PLACEBO COMPARATOR

Participants will consume 1 dose of:15 g maltodextrin (Canadian Protein Maltodextrin) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water

Dietary Supplement: Maltodextrin Placebo

Interventions

Essential Amino Acid DIETARY_SUPPLEMENT

Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement

Essential Amino Acid

Collagen Peptide DIETARY_SUPPLEMENT

Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement

Collagen Peptide

Maltodextrin Placebo DIETARY_SUPPLEMENT

Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement

Maltodextrin Placebo

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Can fluently read and write in English
• Male
• years

You may not qualify if:

• Diabetes, cardiovascular disease, or current pharmaceutical therapy
• Medical conditions impacting ability to undertake strenuous physical activity
• Lower body resistance training in the previous 6 months
• BMI \> 30
• Currently smoking with cigarettes
• Allergy to any study supplements
• Currently taking any protein, amino acid, or collagen supplement
• Participate in greater than 2 hours of weekly vigorous exercise

Sponsors & Collaborators

• University of British Columbia: lead

Study Sites (1)

Chan Gunn Pavilion

Vancouver, British Columbia, V6T 1Z3, Canada

Location

Related Publications (7)

• Shaw G, Lee-Barthel A, Ross ML, Wang B, Baar K. Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. Am J Clin Nutr. 2017 Jan;105(1):136-143. doi: 10.3945/ajcn.116.138594. Epub 2016 Nov 16.

  PMID: 27852613 BACKGROUND

• Holm L, Rahbek SK, Farup J, Vendelbo MH, Vissing K. Contraction mode and whey protein intake affect the synthesis rate of intramuscular connective tissue. Muscle Nerve. 2017 Jan;55(1):128-130. doi: 10.1002/mus.25398. Epub 2016 Sep 26.

  PMID: 27603578 BACKGROUND

• Frontera WR, Ochala J. Skeletal muscle: a brief review of structure and function. Calcif Tissue Int. 2015 Mar;96(3):183-95. doi: 10.1007/s00223-014-9915-y. Epub 2014 Oct 8.

  PMID: 25294644 BACKGROUND

• Egerman MA, Glass DJ. Signaling pathways controlling skeletal muscle mass. Crit Rev Biochem Mol Biol. 2014 Jan-Feb;49(1):59-68. doi: 10.3109/10409238.2013.857291. Epub 2013 Nov 18.

  PMID: 24237131 BACKGROUND

• Holm L, van Hall G, Rose AJ, Miller BF, Doessing S, Richter EA, Kjaer M. Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle. Am J Physiol Endocrinol Metab. 2010 Feb;298(2):E257-69. doi: 10.1152/ajpendo.00609.2009. Epub 2009 Nov 10.

  PMID: 19903866 BACKGROUND

• Alcock RD, Shaw GC, Tee N, Burke LM. Plasma Amino Acid Concentrations After the Ingestion of Dairy and Collagen Proteins, in Healthy Active Males. Front Nutr. 2019 Oct 15;6:163. doi: 10.3389/fnut.2019.00163. eCollection 2019.

  PMID: 31681789 BACKGROUND

• Miller BF, Olesen JL, Hansen M, Dossing S, Crameri RM, Welling RJ, Langberg H, Flyvbjerg A, Kjaer M, Babraj JA, Smith K, Rennie MJ. Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol. 2005 Sep 15;567(Pt 3):1021-33. doi: 10.1113/jphysiol.2005.093690. Epub 2005 Jul 7.

  PMID: 16002437 BACKGROUND

MeSH Terms

Conditions

Motor Activity

Interventions

Amino Acids, Essential

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Amino Acids; Amino Acids, Peptides, and Proteins

Study Officials

• Cameron Mitchell, PhD

  University of British Columbia

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Model Details: \- Double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid)

Study Record Dates

• First Submitted: September 28, 2020
• First Posted: October 22, 2020
• Study Start: January 25, 2021
• Primary Completion: February 13, 2022
• Study Completion: February 13, 2022
• Last Updated: June 13, 2023

Record last verified: 2023-06

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)