Impact of Amino Acids to Enhance Anabolism After Home-based Exercise

NCT04850820 | Completed

• 1 other identifier: B2B
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

An adequate quantity of lean body mass is vital for optimal health and performance, and is accrued when net protein balance (NPB) is positive. Amino acids are the building blocks of protein, and when consumed following resistance exercise, significantly improve NPB. However, no study has investigated how a supplement of all of the essential amino acids (EAA) affects NPB following resistance exercise in a free-living, home-based setting, particularly when compared to a supplement of branched-chain amino acids (BCAA). Therefore, the purpose of this study is to determine the impact of a novel EAA supplement on anabolism (e.g., NPB) as compared to BCAA and placebo supplements following home-based resistance exercise.

Conditions

Amino Acids, Essential; Metabolism; Exercise

Keywords

Home-based resistance exercise; Protein synthesis; Net protein balance; Protein breakdown; Essential amino acids; Branched-chain amino acids; Protein metabolism

Outcome Measures

Primary Outcomes (1)

• Whole-body net leucine balance (umol/kg)

  Used as a proxy of whole-body protein balance, and estimated by using \[13C\]leucine ingestion and breath/urine sample collection

  5 hours/trial (four trials total)

Secondary Outcomes (1)

• Urinary 3-methylhistidine (3-methylhistidine:creatinine ratio)

  5 hours/trial (four trials total)

Study Arms (4)

Novel essential amino acid supplementation (EAA+)

EXPERIMENTAL

Arm will investigate the effect of a novel essential amino acid-based formula (essential amino acids enriched with active botanical compounds; EAA+) developed by Iovate Health Sciences International Inc. on post-exercise anabolism. The formulation was recently granted a Natural Product Number (NPN: 80087022) and approved by the Natural and Non-prescription Health Products Directorate of Health Canada.

Dietary Supplement: Novel essential amino acid supplementation

Branched-chain amino acid supplementation (BCAA)

ACTIVE COMPARATOR

Arm will used a branched-chain amino acid supplement developed by Iovate Health Sciences International Inc. to compare with the experimental arm after exercise.

Dietary Supplement: Branched-chain amino acid supplementation (BCAA)

Isocaloric carbohydrate supplementation

PLACEBO COMPARATOR

Carbohydrate supplement that is isocaloric to the EAA+ supplement and designed to function as a placebo to the experimental arm after exercise.

Dietary Supplement: Isocaloric carbohydrate supplementation

Rested control

OTHER

Carbohydrate supplement that is isocaloric to the EAA+ supplement but consumed at rest to serve as the baseline control

Dietary Supplement: Isocaloric carbohydrate supplementation

Interventions

Novel essential amino acid supplementation DIETARY_SUPPLEMENT

This intervention will determine the impact of EAA+ on markers of whole-body net protein balance, protein synthesis, and protein breakdown following home-based resistance exercise in a free-living setting.

Novel essential amino acid supplementation (EAA+)

Branched-chain amino acid supplementation (BCAA) DIETARY_SUPPLEMENT

This intervention will determine the impact of the BCAA supplement (to be compared to EAA+) on markers of whole-body net protein balance, protein synthesis, and protein breakdown following home-based resistance exercise in a free-living setting.

Branched-chain amino acid supplementation (BCAA)

Isocaloric carbohydrate supplementation DIETARY_SUPPLEMENT

This intervention will determine the impact of an isocaloric carbohydrate placebo supplement (to be compared to EAA+) on markers of whole-body net protein balance, protein synthesis, and protein breakdown following home-based resistance exercise in a free-living setting.

Isocaloric carbohydrate supplementation; Rested control

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Aged 18-35 years
• Recreationally active; currently performing structured exercise (e.g., running, weight lifting, team-sport activity) at least 2 days per week

You may not qualify if:

• Body Mass Index: \>29.9kg/m\^2
• Inability to perform physical activity as determined by the Physical Activity Readiness Questionnaire
• Inability to adhere to protocol guidelines (e.g., alcohol, habitual diet)
• Regular tobacco use
• Illicit drug use (e.g., growth hormone, testosterone)
• Diagnosed medical condition under the care of a physician
• Inability to abstain from supplements (e.g., protein, creatine, HMB, BCAA, phosphatidic acid) at least three weeks before and during trials
• Individuals on any medications known to affect protein metabolism (e.g., corticosteroids, non-steroidal anti-inflammatories, or prescription-strength acne medications)

Sponsors & Collaborators

• University of Toronto: lead
• Iovate Health Sciences International Inc: collaborator

Study Sites (1)

Goldring Centre for High Performance Sport

Toronto, Ontario, M5S2C9, Canada

Location

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Daniel Moore

  University of Toronto

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: All investigators involved with the study, as well as participants, will be blinded to treatment conditions (double-blind design) until data analysis is completed.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Model Details: In a randomized, counterbalanced order, participants will receive all treatments arms (essential amino acids, branched-chain amino acids, and placebo).

Study Record Dates

• First Submitted: April 17, 2021
• First Posted: April 20, 2021
• Study Start: April 1, 2021
• Primary Completion: December 31, 2021
• Study Completion: December 31, 2021
• Last Updated: May 5, 2022

Record last verified: 2022-04

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)