The Effect of a Plant Protein Isolate on Muscle Protein Synthesis in Humans at Rest and After Resistance Exercise

NCT05020808 | Completed

• 1 other identifier: 2020_04_07_EHS
• Study Type: interventional
• Target: 33
• Locations: 1 country
• Sites: 1

Brief Summary

Plant-based proteins contain several amino acids that are essential to signal growth, reconditioning, maintenance and preservation of skeletal muscle mass. This study aims to inform on the nutrient activation of muscle protein synthesis (MPS) by feeding a plant protein isolate equivalent to 0.33 g of protein per kilogram of body mass.

Conditions

Metabolism; Exercise; Dietary Supplements

Keywords

amino acids; deuterium oxide; humans; protein synthesis; plant proteins; resistance training; skeletal muscle

Outcome Measures

Primary Outcomes (1)

• Myofibrillar fractional synthetic rate change

  The fractional rate of myofibrillar protein synthesis measured by deuterium incorporation into skeletal muscle sampled by microbiopsy

  4 hours

Study Arms (2)

Nonessential amino acid formulation (NEAA)

SHAM COMPARATOR

Novel non-essential amino acid blend dosed at 0.33 g/kg body mass in 400 ml of water. Postprandial muscle protein synthesis at rest, and after resistance exercise to be measured by deuterium incorporation in to skeletal muscle sampled by bilateral microbiopsies. Product Number: NEAA56812

Dietary Supplement: NEAA

Plant Protein Isolate (PPI)

ACTIVE COMPARATOR

Plant protein isolate (Fava bean) dosed at 0.33 g/kg body mass in 400 ml of water. Postprandial muscle protein synthesis at rest, and after resistance exercise to be measured by deuterium incorporation in to skeletal muscle sampled by bilateral microbiopsies. Product Number: FP2011273

Dietary Supplement: PPI

Interventions

NEAA DIETARY_SUPPLEMENT

Food grade mixture of non-essential amino acids in powdered form to be dissolved in water. Supplied by Marigot Ltd, Cork, Ireland

Nonessential amino acid formulation (NEAA)

PPI DIETARY_SUPPLEMENT

Food grade plant (legume - Fava) protein isolate to be dissolved in water. Supplied by Marigot Ltd, Cork, Ireland

Also known as: Plant Protein Isolate

Plant Protein Isolate (PPI)

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• \- Men and women aged 18 to 35 years

You may not qualify if:

• Known intolerance to any item contained in the protein products.
• Blood borne disease risk, current illness or medication that would adversely affect participation
• Known adverse reaction to venepuncture and/or biopsy
• Inability to undertake resistance exercise
• Inability to adhere to protocol guidelines

Sponsors & Collaborators

• University of Limerick: lead
• Marigot Ltd.: collaborator
• Enterprise Ireland: collaborator

Study Sites (1)

University of Limerick

Limerick, Munster, V94 T9PX, Ireland

Location

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Philip M Jakeman, PhD

  University of Limerick

  PRINCIPAL INVESTIGATOR

• Robert W Davies, PhD

  University of Limerick

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Masking Details: Single blind (participant)
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Model Details: Block randomized control trial, parallel group design

Study Record Dates

• First Submitted: August 17, 2021
• First Posted: August 25, 2021
• Study Start: September 14, 2021
• Primary Completion: June 20, 2022
• Study Completion: June 20, 2022
• Last Updated: June 22, 2022

Record last verified: 2022-06

Data Sharing

• IPD Sharing: Will not share

Locations

IE (1)