NCT06449222

Brief Summary

This study is a Phase II, multi-site, randomized, open-label clinical study to evaluate the safety, efficacy, and pharmacokinetics (PK) of BNT327 at two dose levels in combination with chemotherapeutic agents in the first- and second-line treatment of participants with locally advanced/metastatic triple-negative breast cancer (mTNBC).

Trial Health

78
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
83

participants targeted

Target at P50-P75 for phase_2

Timeline
37mo left

Started Aug 2024

Longer than P75 for phase_2

Geographic Reach
4 countries

31 active sites

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress36%
Aug 2024Jun 2029

First Submitted

Initial submission to the registry

June 3, 2024

Completed
4 days until next milestone

First Posted

Study publicly available on registry

June 7, 2024

Completed
3 months until next milestone

Study Start

First participant enrolled

August 26, 2024

Completed
3.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

August 1, 2028

Expected
10 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2029

Last Updated

February 25, 2026

Status Verified

February 1, 2026

Enrollment Period

3.9 years

First QC Date

June 3, 2024

Last Update Submit

February 23, 2026

Conditions

Keywords

Programmed Death-1Programmed Death Ligand-1Programmed Death-1 monoclonal antibodiesAnti vascular endothelial growth factor-A (anti-VEGF-A)

Outcome Measures

Primary Outcomes (5)

  • Occurrence of treatment emergent adverse events (TEAEs), adverse events of special interest (AESIs), treatment emergent serious adverse events (SAEs)

    In the combination treatment regimen according to the (US) National Cancer Institute (NCI) Common Terminology Criteria for Adverse Events version 5.0 (CTCAE version 5.0). By treatment arm and overall.

    up to 100 days after the last dose of treatment

  • Occurrence of dose interruption, dose reduction, and discontinuation of study treatment due to adverse events (AEs)

    By treatment arm and overall.

    up to 100 days after the last dose of treatment

  • Objective Response Rate (ORR)

    Defined as the proportion of participants in whom a confirmed complete response (CR) or partial response (PR) (per Response Evaluation Criteria in Solid Tumors version 1.1 \[RECIST 1.1\] based on the investigator's assessment) is observed as best overall response. By treatment arm.

    Until end-of-treatment visit, i.e., up to 24 months after the first dose of treatment

  • Best percentage change from baseline in the tumor size

    Based on the investigator's tumor assessment according to RECIST 1.1. Defined as the change from baseline in percent to the minimal tumor size until tumor progression/recurrence or death (whichever occurs first). By treatment arm.

    Until end-of-treatment visit, i.e., up to 24 months after the first dose of treatment

  • Proportion of participants who have achieved early tumor shrinkage

    Defined as ≥10% decrease in the pretreatment sum of diameters at first post-treatment tumor scan in target lesions. By treatment arm.

    up to 4 months after first dose of treatment

Secondary Outcomes (11)

  • PK assessment: Maximum concentration (Cmax) derived from serum concentration of BNT327

    from pre-dose to 15 days after study treatment

  • PK assessment: Area Under the Curve During the Dosing Interval (AUCtau) derived from serum concentration of BNT327

    from pre-dose to 15 days after study treatment

  • Incidence of detectable BNT327 antidrug antibodies in serum

    from pre-dose to 100 days after last dose of study treatment

  • ORR as assessed by the investigator

    Until end-of-treatment visit, i.e., up to 24 months after the first dose of treatment

  • Duration of Response (DOR)

    Until end-of-treatment visit, i.e., up to 24 months after the first dose of treatment

  • +6 more secondary outcomes

Study Arms (5)

Cohort 1 Arm 1 - BNT327 DL1 + Nab-paclitaxel

EXPERIMENTAL
Drug: BNT327 Dose Level 1 (DL1)Drug: Nab-placlitaxel

Cohort 1 Arm 2 - BNT327 DL2 + Nab-paclitaxel

EXPERIMENTAL
Drug: BNT327 Dose Level 1 (DL2)Drug: Nab-placlitaxel

Cohort 2 Arm 1 - BNT327 Optimized dose + Paclitaxel

EXPERIMENTAL
Drug: PaclitaxelDrug: BNT327 Optimized Dose

Cohort 2 Arm 2 - BNT327 Equivalent Q3W dose + Gemcitabine + Carboplatin

EXPERIMENTAL
Drug: CarboplatinDrug: GemcitabineDrug: BNT327 Equivalent Q3W Dose

Cohort 2 Arm 3 - BNT327 Equivalent Q3W dose + Eribulin

EXPERIMENTAL
Drug: EribulinDrug: BNT327 Equivalent Q3W Dose

Interventions

Intravenous (IV) infusion

Cohort 1 Arm 1 - BNT327 DL1 + Nab-paclitaxel

IV infusion

Cohort 1 Arm 1 - BNT327 DL1 + Nab-paclitaxelCohort 1 Arm 2 - BNT327 DL2 + Nab-paclitaxel

IV infusion

Cohort 1 Arm 2 - BNT327 DL2 + Nab-paclitaxel

IV infusion

Cohort 2 Arm 2 - BNT327 Equivalent Q3W dose + Gemcitabine + Carboplatin

IV infusion

Cohort 2 Arm 2 - BNT327 Equivalent Q3W dose + Gemcitabine + Carboplatin

IV infusion

Cohort 2 Arm 1 - BNT327 Optimized dose + Paclitaxel

IV infusion

Cohort 2 Arm 3 - BNT327 Equivalent Q3W dose + Eribulin

IV infusion

Cohort 2 Arm 1 - BNT327 Optimized dose + Paclitaxel

IV infusion

Cohort 2 Arm 2 - BNT327 Equivalent Q3W dose + Gemcitabine + CarboplatinCohort 2 Arm 3 - BNT327 Equivalent Q3W dose + Eribulin

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Have given informed consent by signing and dating an informed consent form before initiation of any study-specific procedures.
  • Male or female, aged ≥18 years at the time of giving informed consent.
  • Are willing and able to comply with scheduled visits, the treatment schedule, the planned study assessments (including participant completed diaries) and other requirements of the study. This includes that they are able to understand and follow study-related instructions.
  • Have confirmed locally recurrent inoperable or mTNBC as defined by the most recent American Society of Clinical Oncology (ASCO) / College of American Pathologists (CAP) guidelines. Note, participants initially diagnosed with hormone receptor-positive and/or HER2-positive breast cancer must have histological confirmation of TNBC in a tumor biopsy obtained from a local recurrence or distant metastasis site.
  • Systemic treatment naïve locally advanced/metastatic participants are eligible if:
  • They have received no prior systemic therapy in the locally advanced unresectable/metastatic setting including chemotherapy, immunotherapy, or investigational agents.
  • They have completed treatment for Stage I-III breast cancer, if indicated, and ≥6 months has elapsed between the completion of treatment with curative intent (e.g., date of primary breast tumor surgery or date of last adjuvant chemotherapy administration, or date of last radiation therapy, whichever occurred last) and first documented local or distant disease recurrence. This also includes participants initially diagnosed with hormone receptor-positive and/or HER2-positive breast cancer prior to TNBC diagnosis.
  • Participants who received one prior systemic therapy in the locally advanced/metastatic setting are eligible if:
  • They have received one systemic chemotherapy in the metastatic setting and have progressed on first line therapy. Radiographic progression must have been documented. Radiographic progression is defined as unequivocal progression of existing tumor lesions or developing new tumor lesions as assessed by the investigator.
  • They have had a recurrence-free interval of ≥6 months if they have received treatment with curative intent in the past. A recurrence-free interval of ≥6 months is required for all participants (both first- and second-line treatment settings) who have received prior treatment for breast cancer with curative intent.
  • Have provided a tissue sample, archival or fresh, during the screening period (bone biopsies, fine needle aspiration biopsies, and samples from pleural or peritoneal fluid are not acceptable; participants with only one target lesion are not eligible to provide a biopsy). If an archival tumor sample is not available, the participant must undergo a fresh biopsy, if medically feasible to be eligible for the study.
  • Have at least one measurable lesion as the targeted lesion based on RECIST version 1.1. Lesions treated after prior local treatment (radiotherapy, ablation, interventional procedures) are generally not considered as target lesions. If the lesion with prior local treatment is the only targeted lesion, evidence-based radiology must be provided to demonstrate disease progression (the single bone metastasis or the single central nervous system metastasis should not be considered as a measurable lesion).
  • Eastern Cooperative Oncology Group performance status of 0 or 1.
  • Have a minimum life expectancy of \>3 months.
  • Have adequate organ function, as defined below:
  • +17 more criteria

You may not qualify if:

  • Are pregnant or breastfeeding or are planning pregnancy or planning to father children during the study or within 6 months after the last dose of IMP.
  • Have a medical, psychological, or social condition which, in the opinion of the investigator, could compromise their wellbeing if they participate in the study, or that could prevent, limit, or confound the protocol-specified assessments or procedures, or that could impact adherence to protocol described requirements.
  • Have received any of the following therapies or drugs prior to the initiation of study:
  • Participants who received prior treatment with a PD(L)-1/Vascular Endothelial Growth Factor bispecific antibody.
  • Have received a systemic anticancer regimen within 4 weeks prior to the initiation of study treatment or have received palliative radiotherapy within 7 days prior to the initiation of study treatment, or have received any other chemotherapy, curative/palliative radiotherapy, biologic therapy (including tumor vaccines, cytokines, or growth factors for tumor control) or any experimental antitumor drugs within 4 weeks prior to the initiation of study treatment.
  • Have received other systemic immunostimulatory agents or immunosuppressive therapies (such as interferon-alpha \[IFN-α\], interleukin-2 \[IL-2\], or methotrexate) within 4 weeks prior to the initiation of study treatment or are within five half-lives of the treatment drug (whichever is longer). Exception: Excluding local, intranasal, intraocular, intra-articular or inhaled corticosteroids, short-term use (≤7 days) of corticosteroids for prophylaxis (e.g., prevention of contrast agent allergy) or treatment of non-autoimmune conditions (e.g., delayed hypersensitivity reactions caused by exposure to allergens).
  • Have received systemic corticosteroids (at a dosage greater than 10 mg/day of prednisone or an equivalent dose of other corticosteroids) within 3 weeks prior to the initiation of study treatment.
  • Have been vaccinated with live attenuated vaccine(s) within 4 weeks prior to initiation of study treatment.
  • Received broad-spectrum IV antibiotics therapy within 3 weeks prior to initiation of study treatment.
  • Use of any non-study investigational medicinal product within five half-lives of first dose or within 4 weeks, whichever is longer, before initiation of study treatment in this study or ongoing participation in the active treatment phase of another interventional clinical study.
  • Have undergone major organ surgery (core needle biopsies are allowed \>7 days prior study start), significant trauma, or invasive dental procedures (such as dental implants) within 28 days prior to the initiation of study treatment or plan to undergo elective surgery during the study. Placement of vascular infusion devices is allowed.
  • Have received allogeneic hematopoietic stem cell transplantation or organ transplantation.
  • Have spinal cord compression or central nervous system metastases that is untreated and symptomatic or requires treatment with corticosteroids or anticonvulsants for associated-symptom control. Exception: Treated brain metastases which is no longer symptomatic and for which no corticosteroid or anticonvulsant treatment is needed (the participant must be recovered from the acute toxic effect of radiotherapy; study treatment assignment must be ≥2 weeks after completion of radiotherapy).
  • Have active autoimmune disease that has required systemic treatment in the past 2 years (e.g., with use of disease modifying agents, corticosteroids, or immunosuppressive drugs). Replacement therapy (e.g., thyroxine, insulin, or physiologic corticosteroid replacement therapy for adrenal or pituitary insufficiency) is not considered a form of systemic treatment. Those who had a history of autoimmune diseases with anticipated relapse (such as systemic lupus erythematosus, rheumatoid arthritis, vasculitis, etc.), except for those with clinically stable autoimmune thyroid disease or type 1 diabetes.
  • Have had other malignant tumors within 2 years prior to the study treatment are not allowed. Except for those: who have been cured with local treatment (such as basal cell or squamous cell carcinoma of the skin, superficial or non-invasive bladder cancer, carcinoma in situ of the cervix, ductal carcinoma in situ of the breast, and papillary carcinoma of thyroid; including prostate cancer with CR within the past 3 years).
  • +30 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (31)

Beverly Hills Cancer Center

Beverly Hills, California, 90211, United States

Location

Valkyrie Clinical Trials

Los Angeles, California, 90067, United States

Location

Stanford University School of Medicine - Stanford Cancer Institute (SCI) - Stanford Women's Cancer Center

Palo Alto, California, 94304-2201, United States

Location

Saint John's Health Center - John Wayne Cancer Institute (JWCI)

Santa Monica, California, 90404-2312, United States

Location

Rocky Mountain Cancer Centers (RMCC)

Denver, Colorado, 80220, United States

Location

Yale University - Yale Cancer Center

New Haven, Connecticut, 06520, United States

Location

Carle Foundation Hospital d/b/a Carle Cancer Center

Urbana, Illinois, 61801, United States

Location

HealthPartners Regions Specialty Clinics

Saint Louis Park, Minnesota, 55426, United States

Location

Rutgers Cancer Institute of NJ (Rutgers, The State University of New Jersey)

New Brunswick, New Jersey, 08901, United States

Location

Stony Brook University Hospital

Stony Brook, New York, 11794, United States

Location

The West Clinic, P.C. d/b/a West Cancer Center

Germantown, Tennessee, 38138, United States

Location

SCRI Oncology Partners

Nashville, Tennessee, 37203, United States

Location

Bon Secours St. Francis Medical Center

Midlothian, Virginia, 23114, United States

Location

Peninsula Oncology Centre

Frankston, 3199, Australia

Location

Peter MacCallum Cancer Centre

Melbourne, 3050, Australia

Location

Baskent Universitesi Tip Fakultesi Adana Hastanesi

Adana, 01120, Turkey (Türkiye)

Location

Medical Park Seyhan Hospital

Adana, 01230, Turkey (Türkiye)

Location

Sakarya University - Faculty of Medicine

Adapazarı, 54290, Turkey (Türkiye)

Location

Hacettepe Universitesi Kanser Enstitusu

Ankara, 06100, Turkey (Türkiye)

Location

Sbu Dr.A.Y. Ankara Onkoloji SUAM

Ankara, 06100, Turkey (Türkiye)

Location

Memorial Ankara Hospital

Ankara, 06520, Turkey (Türkiye)

Location

Koc Universitesi Hastanesi (Koc University Hospital)

Istanbul, 34010, Turkey (Türkiye)

Location

Bezmialem Vakif Universitesi Tip Fakultesi Hastanesi

Istanbul, 34093, Turkey (Türkiye)

Location

Goztepe Prof. Dr. Suleyman Yalcin Sehir Hastanesi

Istanbul, 34722, Turkey (Türkiye)

Location

Yeditepe University Hospital

Istanbul, 34755, Turkey (Türkiye)

Location

Hull and East Yorkshire Hospitals NHS Trust - Castle Hill Hospital

Cottingham, HU16 5JQ, United Kingdom

Location

Edinburgh Cancer Centre-Western General Hospital

Edinburgh, EH4 2XU, United Kingdom

Location

St James's University Hospital - Leeds Teaching Hospitals NHS Trust

Leeds, LS9 7TF, United Kingdom

Location

St Bartholomew's Hospital - Barts Health NHS Trust

London, EC1A 7BE, United Kingdom

Location

Sarah Cannon Research Institute

London, W1G 6AD, United Kingdom

Location

University College London Hospitals NHS Foundation Trust

London, W1T 7HA, United Kingdom

Location

Related Publications (1)

  • Biteghe FAN, Mungra N, Malindi Z, Chalomie NET, Stanislas KA, Yasmin-Karim S, Mike Makrigiorgos G, Chipidza FE, Akinrinmade OA, Sridhar S, Barth S, Ngwa W. The Therapeutic Potential of Photoimmunotherapy as a Safe, Effective and Non-Toxic Treatment Option for Superficial Triple Negative Breast Cancer. Cancer Med. 2025 Sep;14(18):e71255. doi: 10.1002/cam4.71255.

MeSH Terms

Conditions

Triple Negative Breast Neoplasms

Interventions

CarboplatinGemcitabinePaclitaxeleribulin

Condition Hierarchy (Ancestors)

Breast NeoplasmsNeoplasms by SiteNeoplasmsBreast DiseasesSkin DiseasesSkin and Connective Tissue Diseases

Intervention Hierarchy (Ancestors)

Coordination ComplexesOrganic ChemicalsHeterocyclic CompoundsDeoxycytidineCytidinePyrimidine NucleosidesPyrimidinesHeterocyclic Compounds, 1-RingTaxoidsCyclodecanesCycloparaffinsHydrocarbons, AlicyclicHydrocarbons, CyclicHydrocarbonsDiterpenesTerpenes

Study Officials

  • BioNTech Responsible Person

    BioNTech SE

    STUDY DIRECTOR

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 3, 2024

First Posted

June 7, 2024

Study Start

August 26, 2024

Primary Completion (Estimated)

August 1, 2028

Study Completion (Estimated)

June 1, 2029

Last Updated

February 25, 2026

Record last verified: 2026-02

Data Sharing

IPD Sharing
Will not share

Locations