Metabolic Responses to Exercise and Recovery

NCT06088108 | Unknown DMC

• 1 other identifier: Metabex
• Study Type: interventional
• Target: 48
• Locations: 1 country
• Sites: 1

Brief Summary

Exercise benefits health through diverse metabolic processes and is central to healthy ageing. However, intense exercise also challenges the body, causing cellular damage that must be repaired. This means that we need to identify the level of exercise that can optimise health, and this level might potentially differ by age and sex. Our research aims to tackle this question, by studying the metabolic responses of the body both to exercise and during the subsequent recovery in 48 healthy and active participants between the ages of 8-10, 23-27 and 53-57. Participants will complete an inclusion questionnaire and, if eligible, be invited to visit the exercise laboratory 4-5-hours. We will collect questionnaire data and body measurements before participants undertake exercise on a treadmill. Biological samples (blood from adults only and saliva from everyone) will be collected at three time points (before, right after and one hour after exercise).

Conditions

Exercise; Metabolism

Keywords

Exercise; Metabolism; Recovery; Metabolomics

Outcome Measures

Primary Outcomes (1)

• Concentration of molecules relating to energy metabolism using targeted liquid chromatography-mass spectrometry assays

  Concentrations of metabolites measured in plasma and saliva at baseline, immediately after exercise and one hour after exercise using targeted liquid chromatography-mass spectrometry (LCMS) assays. These include amine, ion-pairing, lipid, fatty acid, acylcarnitine and oxylipin assays.

  At baseline, immediately after exercise and one hour after exercise

Secondary Outcomes (4)

• Concentrations of hormones relating to life history functions using different assays

  At baseline, immediately after exercise and one hour after exercise

• Total body water measurement

  Saliva collection before and 4 hours following consumption of deuterium

• Verbal memory

  Baseline, immediately after exercise and one hour after exercise

• Measurement of stress, mood, appetite perception, and the desire to eat different types of food

  Before breakfast, after breakfast as well as 15, 30, 45 and 60 minutes after exercise

Study Arms (1)

Single arm

EXPERIMENTAL

Study participants are asked to undertake the Cornell treadmill exercise protocol which they complete to voluntary exhaustion.

Device: Cornell treadmill exercise protocol

Interventions

Cornell treadmill exercise protocol DEVICE

Study participants are asked to undertake the Cornell treadmill exercise protocol which they complete to voluntary exhaustion.

Single arm

Eligibility Criteria

• Age: 8 Years - 57 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)

You may qualify if:

• Aged 8-10, 23-27 or 53-57
• Considered healthy without a disease, disability or other condition that would impair participation in physical activity.
• Considered active.
• A body mass index within the ranges of 19 to 24.5 (adults) or the 2nd and 99th percentile (children)

You may not qualify if:

• Outside required age brackets
• Having a disease, disability or other condition that would impair participation in physical activity.
• Having an implanted cardiac pacemaker, defibrillator, or other electronic medical devices.
• Receiving prescription medication one or more days every week over the past 3 months (some medications are permitted).
• Related by blood to another participant.
• Not willing at the time of recruitment to undergo blood sampling (adults only).
• Pregnancy/breastfeeding (adults only).
• Being a professional athlete.
• Not being physically active.
• Being a shift worker (night shift work for three or more days per week on more than two occasions in the six months before the visit day; adults only).
• Recent weight loss of more than 5% of weight in the last 6 months.
• Having a body mass index outside the range of 19 to 24.5 (adults) or outside the 2nd and 99th percentile (children)
• Smoking/vaping.
• Alcohol intake above a UK National Health Service (NHS) questionnaire cut-off.
• Regular use of recreational drugs.

Sponsors & Collaborators

• University College, London: lead
• University of East London: collaborator
• Imperial College London: collaborator

Study Sites (1)

University of East London SportsDock

London, E16 2RD, United Kingdom

RECRUITING

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Jonathan Wells, Professor

  University College, London

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Evelyn Maniaki, Dr

CONTACT

+447481306193; evelyn.maniaki.20@ucl.ac.uk

Andy Galbraith, Dr

CONTACT

A.J.Galbraith@uel.ac.uk

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: October 4, 2023
• First Posted: October 18, 2023
• Study Start: April 1, 2023
• Primary Completion: January 31, 2024
• Study Completion: January 31, 2024
• Last Updated: October 18, 2023

Record last verified: 2023-10

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP, CSR
• Time Frame: It will be available on UCL's repository from March 2025 onwards.

Locations

GB (1)