NCT05800210

Brief Summary

This study will assess the safety, efficacy, and feasibility of ⍺/β CD3+ T-cell and CD19+ B-cell depletion in allogeneic stem cell transplantation in patients with acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), juvenile myelomonocytic leukemia (JMML), high risk myelodysplastic syndrome (MDS), chronic myeloid leukemia (CML) and lymphoma. Subjects will receive an allogeneic stem cell transplant that has been depleted of ⍺/β CD3+ T-cells and CD19+ B-cells using the Miltenyi CliniMACS Prodigy® system.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
20

participants targeted

Target at below P25 for phase_2

Timeline
12mo left

Started May 2024

Typical duration for phase_2

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress67%
May 2024May 2027

First Submitted

Initial submission to the registry

March 23, 2023

Completed
13 days until next milestone

First Posted

Study publicly available on registry

April 5, 2023

Completed
1.1 years until next milestone

Study Start

First participant enrolled

May 3, 2024

Completed
2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 1, 2026

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2027

Expected
Last Updated

June 15, 2025

Status Verified

June 1, 2025

Enrollment Period

2 years

First QC Date

March 23, 2023

Last Update Submit

June 12, 2025

Conditions

Keywords

hematologic malignancyGVHDstem cell transplantationgraft manipulation

Outcome Measures

Primary Outcomes (1)

  • Acute graft versus host disease (aGVHD) incidence

    Compare the incidence of grade II to IV aGVHD following allogeneic stem cell trasplantation utilizing α/β CD3+ T-cell and CD19+ B-cell depletion compared to historical controls by day +100

    100 days

Secondary Outcomes (7)

  • Event-free survival

    2 years

  • Overall survival

    2 years

  • Probability of hematopoietic engraftment

    100 days

  • Cytomegalovirus (CMV) viremia incidence

    1 year

  • Epstein-Barr virus (EBV) viremia incidence

    1 year

  • +2 more secondary outcomes

Study Arms (1)

Allogeneic stem cell transplant with ⍺/β CD3+ T-cell and CD19+ B-cell depleted graft

EXPERIMENTAL
Device: Miltenyi CliniMACS Prodigy ® system

Interventions

Subjects will receive an allogeneic stem cell transplant that has been depleted of ⍺/β CD3+ T-cells and CD19+ B-cells using the Miltenyi CliniMACS Prodigy® system.

Allogeneic stem cell transplant with ⍺/β CD3+ T-cell and CD19+ B-cell depleted graft

Eligibility Criteria

Age6 Months - 39 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • A. Children, Adolescents, Young adults (ages 6 months to ≤39 years) with the following diseases may be eligible:
  • i. ALL
  • ALL high risk including one or more of the following: (t(9;22) or 11q23 chromosomal abnormality, primary induction failure (≤15% blasts at time of registration), mixed phenotype acute leukemia (MPAL), persistent MRD (≥0.01% by flow or persistent abnormal karyotype detected by cytogenetics) or hypodiploidy (≤44 chromosomes)) in first remission
  • ALL in second remission and beyond
  • ii. AML
  • History of AML induction/reinduction Failure (≤15% blasts at time of registration)
  • AML in CR1 with poor cytogenetics (i.e., 12p, 5a, -7, FLT3 mutation/duplication, t(9;11) and others)
  • AML with persistent minimal residual disease (MRD) in CR1(≥0.01% on flow or persistent abnormal karyotype detected by cytogenetics)
  • AML CR2 or beyond
  • AML in refractory relapse but ≤15% bone marrow leukemia blasts
  • Therapy-related AML
  • iii. Juvenile MyeloMonocytic Leukemia (JMML)
  • JMML in CR1 without CBL mutation
  • JMML with recurrence of disease with or without CBL mutation
  • JMML CR2 or beyond
  • +18 more criteria

You may not qualify if:

  • A. Patients with documented uncontrolled infection
  • B. Patients who have received allogeneic hematopoietic stem cell transplantation within 6 months, unless being done as a boost.
  • C. Patients with active ≥Grade 2 aGVHD.
  • D. Demonstrated lack of compliance with medical care.
  • E. Females or males of childbearing potential who are unwilling or unable to use an acceptable method to avoid pregnancy for the entire study period and for at least 8 weeks after the last dose of study drug.
  • F. Females who are known to be pregnant or breastfeeding.
  • G. History of any other disease, metabolic dysfunction, clinical examination finding, or clinical laboratory finding giving reasonable suspicion of a disease or condition that contraindicates the use of protocol therapy or that might affect the interpretation of the results of the study or that puts the subject at high risk for treatment complications, in the opinion of the treating physician.
  • H. Prisoners or subjects who are incarcerated, or subjects who are compulsorily detained for treatment of either a psychiatric or physical illness.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Florida

Gainesville, Florida, 32608, United States

RECRUITING

MeSH Terms

Conditions

Precursor Cell Lymphoblastic Leukemia-LymphomaLeukemia, Myeloid, AcuteLeukemia, Myelomonocytic, JuvenileMyelodysplastic SyndromesLeukemia, Myelogenous, Chronic, BCR-ABL PositiveLymphoma, Non-HodgkinHodgkin DiseaseHematologic Neoplasms

Condition Hierarchy (Ancestors)

Leukemia, LymphoidLeukemiaNeoplasms by Histologic TypeNeoplasmsHematologic DiseasesHemic and Lymphatic DiseasesLymphoproliferative DisordersLymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesLeukemia, MyeloidMyelodysplastic-Myeloproliferative DiseasesBone Marrow DiseasesMyeloproliferative DisordersChronic DiseaseDisease AttributesPathologic ProcessesPathological Conditions, Signs and SymptomsLymphomaNeoplasms by Site

Study Officials

  • Jordan Milner, MD

    University of Florida

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 23, 2023

First Posted

April 5, 2023

Study Start

May 3, 2024

Primary Completion

May 1, 2026

Study Completion (Estimated)

May 1, 2027

Last Updated

June 15, 2025

Record last verified: 2025-06

Locations