Whey Protein Support to Metabolic and Performance Adaptations in Response HIIT

NCT03570424 | Completed

• 1 other identifier: 7867835
• Study Type: interventional
• Target: 35
• Locations: 1 country
• Sites: 1

Brief Summary

High intensity interval training (HIIT) has recently emerged as a time efficient alternative to conventional endurance exercise, conferring similar or superior benefits in terms of metabolic and performance adaptations in both athletic and non-athletic populations. Some of these physiological adaptations include augmented mitochondrial biogenesis and improved substrate metabolism in peripheral tissues such as skeletal muscle. However, nutritional strategies to optimise the adaptations to HIIT have yet to be established. Recent evidence suggests that acute nutritional status can affect the molecular regulation of genes mediating substrate metabolism and mitochondrial biogenesis. Moreover, preliminary evidence suggests that completion of exercise in fasted conditions augments some of these exercise-induced adaptations compared with the fed state. Given the fact that the transient molecular adaptations to acute exercise mediate long-term physiological adaptations, an investigation into the effects of different nutritional interventions on metabolic and performance responses to HIIT is warranted. The purpose of this study is to determine the effects of fasted vs. fed-state (Whey Protein) HIIT on metabolic and performance adaptations in the acute (single exercise session) and chronic (3 weeks, 9 exercise sessions) phases. The primary hypothesis is that different pre-exercise feeding conditions (e.g. fasted placebo vs. Whey protein fed) will result in divergent physiological adaptations in terms of skeletal muscle metabolism and performance, both in response to a single HIIT session and a chronic HIIT intervention.

Conditions

Muscle, Skeletal; High-Intensity Interval Training; Exercise

Keywords

Organelle Biogenesis; Adaptation, Physiological; Whey Proteins; Protein Hydrolysates

Outcome Measures

Primary Outcomes (4)

• Organelle Biogenesis (Mitochondrial) Acute

  Acute phase - change in Peroxisome Proliferator Activated Receptor 1 alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression in response to a single HIIT session. Measured using real-time polymerase chain reaction (RT-PCR).

  Acute - 3 hours post exercise session 1

• Exercise Performance

  Mean power output (Watts) during 20 minute cycling performance test. Measured using cycle ergometer and associated software.

  Chronic - 72 hours post exercise session 9

• Anaerobic Exercise Performance

  Anaerobic exercise performance peak power (Watts). Measured using 30 second Wingate test on a Monark 894E cycle ergometer.

  Chronic - 72 hours post exercise session 9

• Organelle Biogenesis (Mitochondrial) Chronic

  Chronic Phase - change in Citrate Synthase Activity measured using commercially available assay kits.

  Chronic - 48 hours post exercise session 9

Secondary Outcomes (2)

• Organelle Biogenesis (Mitochondrial)

  Acute: 3 hours post HIIT session 1.

• Cycling Economy

  Chronic - 72 hours post exercise session 9

Study Arms (3)

Placebo

PLACEBO COMPARATOR

Intervention (Nutrient support to HIIT): Participants consume 0.33g.kg-1 body mass of artificially flavoured and textured placebo 45 minutes prior to HIIT exercise

Dietary Supplement: Placebo

Whey Protein

EXPERIMENTAL

Intervention (Nutrient support to HIIT): Participants consume 0.33g.kg-1 body mass intact whey protein 45 minutes prior to HIIT exercise

Dietary Supplement: Nutrient support to HIIT

Whey Protein Hydrolysate

EXPERIMENTAL

Intervention (Nutrient support to HIIT): Participants consume 0.33g.kg-1 body mass hydrolysed whey protein 45 minutes prior to HIIT exercise

Dietary Supplement: Nutrient support to HIIT

Interventions

Nutrient support to HIIT DIETARY_SUPPLEMENT

3 groups of young (aged 18-35 y), healthy, recreationally active, aerobically untrained (VO2max \<50 ml.kg.min-1) males will undertake 3 weeks (9 sessions) of HIIT under different nutrient conditions following \>10h overnight fast: i) Placebo: Fasted artificially flavoured and textured placebo 45 minutes prior to exercise; ii) Whey protein 45 minutes prior to exercise; iii) Whey protein hydrolysate 45 minutes prior to exercise).

Whey Protein; Whey Protein Hydrolysate

Placebo DIETARY_SUPPLEMENT

3 groups of young (aged 18-35 y), healthy, recreationally active, aerobically untrained (VO2max \<50 ml.kg.min-1) males will undertake 3 weeks (9 sessions) of HIIT under different nutrient conditions following \>10h overnight fast: i) Fasted artificially flavoured and textured placebo 45 minutes prior to exercise; ii) Fed Whey protein 45 minutes prior to exercise; iii) Fed Whey protein hydrolysate 45 minutes prior to exercise).

Placebo

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy (absence of clinical condition)
• Recreationally active
• Aerobically untrained (VO2max \<50 ml.kg.min-1)
• Protein sufficient (\>0.8 g.kg.d-1)
• Males
• Able to provide informed consent
• No contraindications to high intensity exercise

You may not qualify if:

• BMI \>30 kg.m-2
• Metabolic disease (mitochondrial, Type 2 Diabetes)

Sponsors & Collaborators

• University of Limerick: lead

Study Sites (1)

University of Limerick

Limerick, Munster, V94 T9PX, Ireland

Location

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Brian P Carson, PhD

  University of Limerick

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Masking Details: Participants are block randomised to one of three nutrient conditions on provision of informed consent. This information is held by the PI and members of the research team independent of the outcomes assessor (masked). All three beverages are made up by members of the research team independent of the outcomes assessor (masked) and the participants (masked). Each participant (masked) is provided with a drink in a black, non-transparent, container with no details of its contents other than that is a "nutrient supplement".
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Lecturer in Exercise Physiology, University of Limerick

Model Details: A randomly assigned, parallel group, simple pre-post design has been adopted to answer this question. 3 groups of young (aged 18-35 y), healthy, recreationally active, aerobically untrained (VO2max \<50 ml.kg.min-1) males will undertake 3 weeks (9 sessions) of HIIT under different nutrient conditions following \>10h overnight fast: i) Fasted placebo (0.33g.kg-1 body artificially flavoured and textured placebo); ii) Fed Whey protein (0.33g.kg-1 body mass intact whey protein 45 minutes prior to exercise); iii) Fed Whey protein hydrolysate (0.33g.kg-1 body mass hydrolysed whey protein 45 minutes prior to exercise). Participants will undergo biological sampling (venous blood and muscle biopsy) and measures of performance pre and post the intervention.

Study Record Dates

• First Submitted: May 22, 2018
• First Posted: June 27, 2018
• Study Start: January 31, 2018
• Primary Completion: February 1, 2019
• Study Completion: February 1, 2019
• Last Updated: January 13, 2020

Record last verified: 2020-01

Data Sharing

• IPD Sharing: Will not share

Locations

IE (1)