NCT03166254

Brief Summary

Both metastatic squamous non-small cell lung cancer (NSCLC) and extensive stage small cell lung cancer (SCLC) are incurable with current therapies, but due to mutations induced by cigarette smoke, typically express a large number of altered proteins that can be recognized as foreign by the immune system. This antigenicity is thought to explain the efficacy of pembrolizumab as either a first or second line treatment in this disease. For patients who receive chemotherapy plus immunotherapy as a first line therapy, there is sound rationale for combination treatment with immunotherapy and a therapeutic antitumor vaccine as a maintenance strategy. Regardless of PD-L1 expression in the tumor, monoclonal antibodies that block PD-1/PD-L1 interactions are effective second line therapies after chemotherapy in both NSCLC and SCLC. In addition, by targeting the immune system against tumor specific antigens using a peptide vaccine, the efficacy of pembrolizumab alone is expected to be enhanced, with an improved response rate and prolonged overall survival with no additional toxicity. This pilot study will provide a preliminary test of the feasibility of generating a personalized, tumor neoantigen-specific therapeutic vaccine and the safety of combining it with checkpoint blockade immunotherapy.

Trial Health

45
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Timeline
13mo left

Started Apr 2019

Longer than P75 for phase_1

Status
withdrawn

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress87%
Apr 2019May 2027

First Submitted

Initial submission to the registry

May 23, 2017

Completed
2 days until next milestone

First Posted

Study publicly available on registry

May 25, 2017

Completed
1.9 years until next milestone

Study Start

First participant enrolled

April 30, 2019

Completed
3.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 30, 2022

Completed
4.9 years until next milestone

Study Completion

Last participant's last visit for all outcomes

May 31, 2027

Expected
Last Updated

April 12, 2019

Status Verified

April 1, 2019

Enrollment Period

3.2 years

First QC Date

May 23, 2017

Last Update Submit

April 10, 2019

Conditions

Outcome Measures

Primary Outcomes (1)

  • Safety and feasibility of the combined regimen as measured by number of participants who experience a serious adverse event

    -The descriptions and grading scales found in the revised NCI Common Terminology Criteria for Adverse Events (CTCAE) version 5.0 will be utilized for all toxicity reporting.

    30 days following the completion of treatment (estimated to be 2 years and 16 weeks)

Secondary Outcomes (8)

  • Objective response rate as measured by RECIST 1.1

    Through completion of treatment (estimated to be 108 weeks)

  • Progression-free survival (PFS) as measured by RECIST 1.1

    Through completion of follow-up (estimated to be 7 years)

  • Overall survival (OS)

    Through completion of follow-up (estimated to be 7 years)

  • Clinical benefit rate (CBR)

    Through completion of treatment (estimated to be 108 weeks)

  • Duration of response (DOR)

    Through completion of treatment (estimated to be 108 weeks)

  • +3 more secondary outcomes

Study Arms (2)

Cohort A: Stage IV squamous NSCLC

EXPERIMENTAL

* 4 cycles of standard of care (SOC) platinum doublet chemotherapy (investigator's choice) * Pembrolizumab (200 mg every 3 weeks (Q3W) for 4 cycles while patient is receiving SOC chemo). Pembrolizumab may continue to be administered Q3W for a maximum of 35 total administrations * All patients will begin the NEO-PV-01 vaccination at Week 12, 3 weeks following the 4th cycle of pembrolizumab / chemotherapy. At Week 12 (Cycle 5), NEO-PV-01 vaccinations will be administered in a prime-boost schedule with 5 priming vaccinations over a 3 week period followed by booster vaccinations at 1 month and 2 months after the last priming vaccination * Up to 20 personalized vaccine peptides will be administered for each patient. Vaccine administration will occur on Days 1, 4, 8, 15 during Cycle 5 of pembrolizumab administration and Day 1 of Cycle 6 of pembrolizumab administration, on Day 8 during Cycle 7 of pembrolizumab administration, and on Day 15 during Cycle 8 of pembrolizumab administration

Drug: PembrolizumabBiological: NEO-PV-01 vaccineProcedure: BiopsyDrug: Poly ICLCProcedure: LeukapheresisProcedure: Peripheral blood samples

Cohort B: Extensive stage SCLC

EXPERIMENTAL

* 4 cycles of standard of care (SOC) platinum doublet chemotherapy (investigator's choice) * Pembrolizumab (200 mg every 3 weeks (Q3W) for 4 cycles while patient is receiving SOC chemo). Pembrolizumab may continue to be administered Q3W for a maximum of 35 total administrations * All patients will begin the NEO-PV-01 vaccination at Week 12, 3 weeks following the 4th cycle of pembrolizumab / chemotherapy. At Week 12 (Cycle 5), NEO-PV-01 vaccinations will be administered in a prime-boost schedule with 5 priming vaccinations over a 3 week period followed by booster vaccinations at 1 month and 2 months after the last priming vaccination * Up to 20 personalized vaccine peptides will be administered for each patient. Vaccine administration will occur on Days 1, 4, 8, 15 during Cycle 5 of pembrolizumab administration and Day 1 of Cycle 6 of pembrolizumab administration, on Day 8 during Cycle 7 of pembrolizumab administration, and on Day 15 during Cycle 8 of pembrolizumab administration

Drug: PembrolizumabBiological: NEO-PV-01 vaccineProcedure: BiopsyDrug: Poly ICLCProcedure: LeukapheresisProcedure: Peripheral blood samples

Interventions

-Pembrolizumab will be given intravenously over the course of 30 minutes

Also known as: Keytruda
Cohort A: Stage IV squamous NSCLCCohort B: Extensive stage SCLC

-Generation of the vaccine is expected to take approximately 12 weeks

Also known as: Personalized vaccine
Cohort A: Stage IV squamous NSCLCCohort B: Extensive stage SCLC
BiopsyPROCEDURE

-Surgical or core needle biopsy of an accessible site for DNA and RNA sequencing, immunological analysis, and generation of NEO-PV-01 vaccine

Cohort A: Stage IV squamous NSCLCCohort B: Extensive stage SCLC

-NEO-PV-01 is combined with the adjuvant poly-ICLC prior to administration.

Also known as: Hiltonol
Cohort A: Stage IV squamous NSCLCCohort B: Extensive stage SCLC
LeukapheresisPROCEDURE

-Peripheral blood mononuclear cells (PBMCs) for comprehensive immune system monitoring will be obtained from leukapheresis samples collected up to 7 days prior to initiation of NEO PV-01 vaccination and at 7 days (Week 20) following the first NEO-PV-01 booster vaccination.

Cohort A: Stage IV squamous NSCLCCohort B: Extensive stage SCLC

-Blood samples (80 mL) for immune monitoring will be obtained prior to study treatment and at Weeks 6, 14, 16, 24, 36, 48, 63, 75, 87, and 99.

Cohort A: Stage IV squamous NSCLCCohort B: Extensive stage SCLC

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Cohort A: Histologically confirmed stage IV squamous NSCLC
  • Cohort B: Histologically confirmed extensive stage SCLC
  • Sufficient tumor tissue must be available for histologic assessment of PD-L1 expression and for sequence and immunological analysis.
  • Measurable disease by RECIST 1.1. Lesions situated in a previously irradiated area are considered measurable if progression has been demonstrated in such lesions.
  • At least 18 years of age on the day of signing informed consent.
  • Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1
  • Normal bone marrow and organ function as defined in the table below within 10 days of study entry:
  • Absolute neutrophil count (ANC): ≥1500/µL
  • Platelets: ≥100 000/µL
  • Hemoglobin: ≥9.0 g/dL or ≥5.6 mmol/L (Criteria must be met without erythropoietin dependency and without packed red blood cell (pRBC) transfusion within last 2 weeks)
  • Creatinine: ≤1.5 × upper limit of normal (ULN) OR
  • Measured or calculated2 creatinine clearance (GFR can also be used in place of creatinine or CrCl): ≥30 mL/min for patient with creatinine levels / \>1.5 × institutional ULN
  • Total bilirubin: ≤1.5 ×ULN OR direct bilirubin ≤ULN for patients with total bilirubin levels \>1.5 × ULN
  • AST (SGOT) and ALT (SGPT): ≤2.5 × ULN (≤5 × ULN for patients with liver metastases)
  • International normalized ratio (INR) OR prothrombin time (PT) activated partial thromboplastin time (aPTT): ≤1.5 × ULN unless patient is receiving anticoagulant therapy as long as PT or aPTT is within therapeutic range of intended use of anticoagulants
  • +3 more criteria

You may not qualify if:

  • Cohort A: Received any prior systemic therapy for cancer treatment.
  • Cohort B: May not have received more than one cycle of platinum doublet given with or without an anti-PD-1 or anti-PD-L1 immunotherapeutic.
  • Prior chemotherapy, targeted small molecule therapy, or radiation therapy within 14 days prior to day 1 or who has not recovered (ie, ≤ Grade 1 or at baseline from adverse events due to previous therapies). Patients with ≤Grade 2 neuropathy may be eligible.
  • Note: If patient received major surgery, they must have recovered adequately from the toxicity and/or complications from the intervention prior to starting study treatment.
  • Received prior radiotherapy within 2 weeks of start of study treatment. Patients must have recovered from all radiation-related toxicities, not require corticosteroids, and not have had radiation pneumonitis. A 1-week washout is permitted for palliative radiation (≤2 weeks of radiotherapy) to non-CNS disease.
  • Patients may not receive or have received any radiation therapy at the biopsy sites.
  • Is currently participating in or has participated in a study of an investigational agent or has used an investigational device within 4 weeks prior to the first dose of study treatment.
  • Note: Patients who have entered the follow-up phase of an investigational study may participate as long as it has been 4 weeks after the last dose of the previous investigational agent.
  • Has had an allogeneic tissue/solid organ transplant.
  • Has a diagnosis of immunodeficiency or is receiving systemic steroid therapy \> 10 mg prednisone daily or any other form of immunosuppressive therapy within 7 days prior to Day 1.
  • Prior treatment with an anti-PD-1, anti-PD-L1, or anti-PD-L2 agent.
  • Prior treatment with a cancer vaccine.
  • Prior treatment with an agent directed to another stimulatory or co-inhibitory T-cell receptor (eg, CTLA-4, OX-40, CD137).
  • Received a live vaccine within 30 days prior to Day 1. Examples of live vaccines include, but are not limited to, the following: measles, mumps, rubella, varicella/zoster (chicken pox), yellow fever, rabies, Bacillus Calmette-Guérin (BCG), and typhoid vaccine. Seasonal influenza vaccines for injection are generally killed virus vaccines and are allowed; however, intranasal influenza vaccines (eg, FluMist®) are live attenuated vaccines and are not allowed.
  • Known active CNS metastases and/or carcinomatous meningitis. Subjects with previously treated brain metastases may participate provided they are stable (without evidence of progression by imaging for at least four weeks prior to Day 1 and any neurologic symptoms have returned to baseline), have no evidence of new or enlarging brain metastases, and are not using steroids for at least 7 days prior to trial treatment.
  • +17 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Links

MeSH Terms

Conditions

Carcinoma, Non-Small-Cell Lung

Interventions

pembrolizumabBiopsypoly ICLCLeukapheresis

Condition Hierarchy (Ancestors)

Carcinoma, BronchogenicBronchial NeoplasmsLung NeoplasmsRespiratory Tract NeoplasmsThoracic NeoplasmsNeoplasms by SiteNeoplasmsLung DiseasesRespiratory Tract Diseases

Intervention Hierarchy (Ancestors)

CytodiagnosisCytological TechniquesClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisSpecimen HandlingDiagnostic Techniques, SurgicalSurgical Procedures, OperativeInvestigative TechniquesCytapheresisBiological TherapyTherapeuticsBlood Component RemovalLeukocyte Reduction ProceduresCell Separation

Study Officials

  • Ramaswamy Govindan, M.D.

    Washington University School of Medicine

    PRINCIPAL INVESTIGATOR
0

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 23, 2017

First Posted

May 25, 2017

Study Start

April 30, 2019

Primary Completion

June 30, 2022

Study Completion (Estimated)

May 31, 2027

Last Updated

April 12, 2019

Record last verified: 2019-04