Identification of Novel Skeletal Muscle-derived Factors That Promote Lipid Oxidation (Columbus)

NCT01911091 | Active Not Recruiting DMC

• 1 other identifier: TRIMDFH 460196
• Study Type: interventional
• Target: 56
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to collect data to help researchers identify factors, such as certain proteins or genetic codes, that are secreted from muscle that are associated with the beneficial effects of exercise.

Conditions

Obesity; Metabolic Disorder; Disorder of Lipid Storage and Metabolism; Lipid Metabolism Disorders

Keywords

skeletal muscle; adipose tissue; metabolism; oxidation; mitochondrial capacity

Outcome Measures

Primary Outcomes (1)

• Measure change in mitochondrial capacity

  The difference will be measured in obese, lean and athletic participants. The Phosphocreatine (PCr) recovery time constant and the PCr level in oxygenated muscle at rest will be used to calculate maximum mitochondrial capacity.

  Baseline (Day -6), Day 18

Secondary Outcomes (2)

• Measure change of expression of proteins

  Baseline (Day -6), Day 0, Day 5, Day 12, Day 18

• Measure change in mRNA/miRNA levels

  Baseline (Day -6), Day 0, Day 5, Day 12, Day 18

Study Arms (3)

Group 1 - Regular exercise

EXPERIMENTAL

Alternate interval training and aerobic training and exercise

Behavioral: Exercise

Group 2 - Athlete exercise

NO INTERVENTION

Athletes are not given any intervention

Group 3 - Obese No Exercise

NO INTERVENTION

The Obese group will not receive intervention

Interventions

Exercise BEHAVIORAL

A 5-minute warm-up and a 5-minute cool-down prior to and following each exercise session, respectively. There will be alternating days of interval training and aerobic training. The interval training will be performed on an upright stationary bike, while the aerobic training will be performed on a treadmill. The interval training will consist of five-minute bouts of higher intensity alternated with 4 minutes of lower intensity for a total duration of 45 minutes. Intensity will increase each week. The aerobic training component will be fixed at a moderate intensity, but will increase in duration each week from 45 minutes to 75 minutes to 90 minutes during the third and final week.

Group 1 - Regular exercise

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Applicable to all Groups
• Healthy men and women, aged 18 - 40, inclusive.
• Willing to stop alcohol and caffeine consumption for 48 hours preceding each blood draw
• Applicable to Group 1
• BMI between 22 and 29.9 kg/m2
• Not involved in regular exercise program
• Willing to exercise every day for the study period
• Applicable to Group 2
• BMI between 22 and 29.9 kg/m2
• Maximal oxygen uptake (VO2max) ≥ 45 ml/kg fat-free mass
• /min
• Engaged in a minimum of 1.5 h of moderate to vigorous intensity aerobic exercise 3 times/ week
• Applicable to Group 3
• BMI ≥ 30 kg/m2 and weight ≤ 350 lbs
• Not involved in a regular exercise program

You may not qualify if:

• Applicable to All Groups
• History of Type 2 Diabetes
• "Unfavorable anatomy" for continuous venous blood sample collection
• Abnormal resting ECG
• Significant renal, cardiac, liver, lung, or neurological disease (controlled hypertension is acceptable if baseline bp \< 140/90 on medications)
• Use of drugs known to affect energy metabolism or body weight: including, but not limited to: orlistat, sibutramine, ephedrine, phenylpropanolamine, corticosterone, etc
• Current treatment with blood thinners or anti-platelet medications that cannot be safely stopped for testing procedures
• New onset (\<3 months on a stable regime) use of oral contraceptives or hormone replacement therapy
• Alcohol or other drug abuse
• Smoking within the past 3 months
• Females that are currently or have been pregnant or are currently or have nursed a child within the last 12 months (minimum).
• Parental enrollment into the study that compromises the well being of the child \[no partner or connected caregiver\]
• Unwilling or unable to abstain from caffeine or alcohol 48 hours prior to metabolic rate measurements
• Increased liver function tests
• Metal objects that would interfere with the measurement of body composition /magnetic resonance spectroscopy such as implanted rods, surgical clips, etc

Sponsors & Collaborators

• AdventHealth Translational Research Institute: lead
• Sanford-Burnham Medical Research Institute: collaborator
• Takeda: collaborator

Study Sites (1)

Translational Research Institute for Metabolism and Diabetes

Orlando, Florida, 32804, United States

Location

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Related Links

• Florida Hospital's Translational Research Institute for Metabolism and Diabetes

MeSH Terms

Conditions

Obesity; Lipid Metabolism Disorders; Metabolic Diseases

Interventions

Exercise

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Lauren Sparks, PhD

  Translational Research Institute for Metabolism and Diabetes

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: July 24, 2013
• First Posted: July 30, 2013
• Study Start: July 1, 2013
• Primary Completion: December 1, 2014
• Study Completion (Estimated): December 1, 2026
• Last Updated: March 30, 2026

Record last verified: 2026-03

Locations

US (1)