Exercise and Insulin Signaling in Human Skeletal Muscle

NCT02987491 | Completed

• 1 other identifier: 7605
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Obesity is associated with a decrease in skeletal muscle insulin sensitivity. Aerobic exercise can increase insulin sensitivity in the few hours following exercise, however the cellular mechanisms are not completely understood. The current project is to investigate mechanisms of exercise improvements to skeletal muscle insulin sensitivity.

Conditions

Insulin Sensitivity; Obesity; Sedentary Lifestyle

Keywords

exercise

Outcome Measures

Primary Outcomes (1)

• Skeletal muscle insulin sensitivity

  Skeletal muscle insulin sensitivity will be measured by hyperinsulinemic-euglycemic clamp.

  2 hours following rest or exercise

Study Arms (2)

Exercise Metabolic Study Day

EXPERIMENTAL

Participants will perform a single bout of moderate intensity exercise on a cycle ergometer for 60 minutes. A total of 3 muscle biopsies will be collected throughout the study day. Insulin sensitivity will be measured using a hyperinsulinemic-euglycemic clamp with glucose tracers.

Behavioral: Exercise

Resting Metabolic Study Day

NO INTERVENTION

Participants will rest quietly in bed for 60 minutes and resting energy metabolism will be measured with a ventilated hood. A total of 3 muscle biopsies will be collected throughout the study day. Insulin sensitivity will be measured using a hyperinsulinemic-euglycemic clamp with glucose tracers.

Interventions

Exercise BEHAVIORAL

Participants will perform 2 metabolic study days of either resting or acute bout of cycling exercise in a randomized cross-over design.

Exercise Metabolic Study Day

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Males or Females
• Aged 18-45 years
• Normal weight (body mass index \[BMI\] 18-26 kg/m2) or obese (BMI 30-46 kg/m2)
• Sedentary (\< 1 hour of planned physical activity per week for ≥ 6 months)
• Weight stable (\< 2 kg change in body mass for ≥ 6 months)
• Non-smokers (no tobacco or nicotine use for ≥ 1 year)

You may not qualify if:

• Hypertension (systolic blood pressure \>140 mmHg, diastolic blood pressure \> 90mmHg)
• Chronic health condition including diabetes, cardiovascular disease, treated hypertension, cancer, anemia, uncontrolled hyper- or hypothyroidism.
• Pregnant, nursing, irregular menses or post-menopausal (if female)
• Hyperglycemia (fasting glucose \>126 mg/dl)
• Hypercholesterolemia (fasting LDL\>140mg/dl)
• Hemoglobin \< 13.0 g/dl (males), \< 11.5 g/dl (females)
• Compromised renal function (outside 135-145 mmol/L sodium, 3.5-5.1 mmol/L potassium)
• Lidocaine allergy
• Medications including β-blockers, angiotensin converting enzyme inhibitors, insulin, thiazolidinediones, metformin, sulfonylureas, chronic non-steroidal anti-inflammatory use, anti-coagulant (e.g. warfarin), current antibiotics, opiates, monoamine oxidase inhibitors, benzodiazepines, or others that may impact the study outcomes
• Any physical limitation that prevents a participant from safely completing the exercise test
• Due to the risks associated with the current protocol, individuals with a diminished capacity to consent will be excluded. Similarly, due to the need for constant, accurate participant monitoring during metabolic study activities, participants will need to clearly understand verbal and written English. Participants who cannot clearly understand verbal and written English will be excluded.

Sponsors & Collaborators

• Oregon State University: lead

Study Sites (1)

Oregon State University

Corvallis, Oregon, 97331, United States

Location

Related Publications (2)

• Newsom SA, Stierwalt HD, Ehrlicher SE, Robinson MM. Substrate-Specific Respiration of Isolated Skeletal Muscle Mitochondria after 1 h of Moderate Cycling in Sedentary Adults. Med Sci Sports Exerc. 2021 Jul 1;53(7):1375-1384. doi: 10.1249/MSS.0000000000002615.

  PMID: 34127633 DERIVED

• Stierwalt HD, Ehrlicher SE, Robinson MM, Newsom SA. Skeletal Muscle ACSL Isoforms Relate to Measures of Fat Metabolism in Humans. Med Sci Sports Exerc. 2021 Mar 1;53(3):624-632. doi: 10.1249/MSS.0000000000002487.

  PMID: 32796254 DERIVED

MeSH Terms

Conditions

Insulin Resistance; Obesity; Sedentary Behavior; Motor Activity

Interventions

Exercise

Condition Hierarchy (Ancestors)

Hyperinsulinism; Glucose Metabolism Disorders; Metabolic Diseases; Nutritional and Metabolic Diseases; Overweight; Overnutrition; Nutrition Disorders; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Behavior

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Matthew Robinson, PhD

  Oregon State University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 21, 2016
• First Posted: December 9, 2016
• Study Start: November 1, 2016
• Primary Completion: June 28, 2018
• Study Completion: June 28, 2018
• Last Updated: August 28, 2019

Record last verified: 2019-08

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)