The Effects of Acute Total Sleep Deprivation Versus Normal Sleep on Metabolism

NCT01800253 | Completed

• 1 other identifier: SleepMetaJCCB2013
• Study Type: interventional
• Target: 17
• Locations: 1 country
• Sites: 1

Brief Summary

The study proposes to investigate whether acute total deprivation affects metabolism as measured through blood and peripheral tissues. Its aim is also to investigate how acute total sleep deprivation affects neurodegenerative markers, as well as hormones, memory performance and aspects of appetite regulation.

Conditions

Sleep Deprivation; Sleep

Outcome Measures

Primary Outcomes (1)

• Gene expression and DNA methylation

  This study has been designed to measure the changes in gene expression and DNA methylation in circulating blood, i.e. mainly of white blood cells with active transcription and DNA regulation, and how this relates to possible changes in peripheral tissues involved in metabolism.

  Change from baseline (ie. around 1930 in the evening - before sleep intervention) to 12 hours later (around 0730 in the morning after the nighttime intervention), and to 15 hours later (around 1030 in the morning after the nighttime intervention)

Secondary Outcomes (4)

• Circulating hormone and neuromolecular levels

  Change in circulating hormone levels from baseline (ie. around 1930 in the evening - before sleep intervention) to 12 hours later (around 0730 in the morning after the nighttime intervention)

• Appetitive evaluation

  Change in appetitive ratings after the sleep intervetion (from around 0700 in the morning after the nighttime intervention), repeated each hour

• Portion Size Task

  Change in selected portion size in the morning, at around 0830 hours in the morning following each nighttime intervention, and 2h30min later, i.e. 30 min after consuming an Oral glucose tolerance test, i.e. around 1100 hours

• Inhibitory task

  Change in cognitive inhibitory performance at around 0810 hours in the morning following the respective nighttime intervention.

Study Arms (2)

Total sleep deprivation

EXPERIMENTAL

Participants will be required to stay up for the entire night before 'Blood Samples' and 'Tissue samples' will be taken and the 'Portion Size Task' and 'Inhibitory task' will be performed. This will then be followed by the 'Oral glucose tolerance test' with additional 'Blood Samples' to be taken as described for that test.

Behavioral: Inhibitory task; Procedure: Blood samples; Procedure: Tissue samples; Procedure: Oral glucose tolerance test; Behavioral: Portion Size Task

Sleep

EXPERIMENTAL

Participants will have an 8-h sleep opportunity before 'Blood Samples' and 'Tissue samples' will be taken and 'Portion Size Task' and 'Inhibitory task' will be performed. This will be followed by the 'Oral glucose tolerance test' with additional 'Blood Samples' to be taken as described for that test.

Behavioral: Inhibitory task; Procedure: Blood samples; Procedure: Tissue samples; Procedure: Oral glucose tolerance test; Behavioral: Portion Size Task

Interventions

Inhibitory task BEHAVIORAL

Participants perform a binary decision on each presented stimuli. Of the two possible outcomes, participants are instructed to make a motor response (go) for one type, and are to withhold a response (no-go) for the other type. Reaction time and accuracy are measured for each event

Sleep; Total sleep deprivation

Blood samples PROCEDURE

Hormone levels, neuromolecular levels and gene expression profiles will be analyzed from repeated blood samples obtained before and after the nighttime intervention

Sleep; Total sleep deprivation

Tissue samples PROCEDURE

Expression profiles will be analyzed from samples obtained from tissues involved in metabolism

Sleep; Total sleep deprivation

Oral glucose tolerance test PROCEDURE

75 g of glucose will be dissolved in 300 ml of water and given to participants, followed by blood sampling at 0, 15, 30, 60, 90, 120 and 150 minutes following the ingestion of the glucose solution.

Sleep; Total sleep deprivation

Portion Size Task BEHAVIORAL

Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume

Sleep; Total sleep deprivation

Eligibility Criteria

• Age: 18 Years - 28 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male
• Age 18-28y
• Healthy (self-reported) and not on medication
• Non-smoking
• Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported via diaries)

You may not qualify if:

• Major illness
• Taking any serious medications
• Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
• Any dietary issues with the food items provided
• Current or history of endocrine, neurological or psychiatric disorders
• Shift work in the preceding three months or for a long duration
• Time travel over a significant number of time zones in the preceding two months
• Too much weight gain or weight loss in the preceding three months

Sponsors & Collaborators

• Uppsala University: lead

Study Sites (1)

Department of Neuroscience, Uppsala University

Uppsala, Uppsala County, 75324, Sweden

Location

Related Publications (3)

• Zhang L, Grip A, Hjelmqvist D, Benedict C, Brandao LEM, Cedernaes J. Acute Sleep Loss Increases Circulating Morning Levels of Two MicroRNAs Implicated in Neurodegenerative Disease in Healthy Young Men. J Cell Mol Med. 2025 Apr;29(7):e70523. doi: 10.1111/jcmm.70523.

  PMID: 40194981 DERIVED

• Mateus Brandao LE, Espes D, Westholm JO, Martikainen T, Westerlund N, Lampola L, Popa A, Vogel H, Schurmann A, Dickson SL, Benedict C, Cedernaes J. Acute sleep loss alters circulating fibroblast growth factor 21 levels in humans: A randomised crossover trial. J Sleep Res. 2022 Apr;31(2):e13472. doi: 10.1111/jsr.13472. Epub 2021 Sep 2.

  PMID: 34476847 DERIVED

• Cedernaes J, Osler ME, Voisin S, Broman JE, Vogel H, Dickson SL, Zierath JR, Schioth HB, Benedict C. Acute Sleep Loss Induces Tissue-Specific Epigenetic and Transcriptional Alterations to Circadian Clock Genes in Men. J Clin Endocrinol Metab. 2015 Sep;100(9):E1255-61. doi: 10.1210/JC.2015-2284. Epub 2015 Jul 13.

  PMID: 26168277 DERIVED

MeSH Terms

Conditions

Sleep Deprivation

Interventions

Blood Specimen Collection; Glucose Tolerance Test

Condition Hierarchy (Ancestors)

Dyssomnias; Sleep Wake Disorders; Nervous System Diseases; Neurologic Manifestations; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Mental Disorders

Intervention Hierarchy (Ancestors)

Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Investigative Techniques; Blood Chemical Analysis; Clinical Chemistry Tests; Diagnostic Techniques, Endocrine

Study Officials

• Christian Benedict, PhD

  Department of Neuroscience, Uppsala University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 21, 2013
• First Posted: February 27, 2013
• Study Start: March 1, 2013
• Primary Completion: October 1, 2013
• Study Completion: October 1, 2013
• Last Updated: December 9, 2013

Record last verified: 2013-11

Locations

SE (1)