Sleep Deprivation's Regulation of Immune System Function and Behavior

NCT01730742 | Unknown

• 1 other identifier: 192837465
• Study Type: interventional
• Target: 18
• Locations: 1 country
• Sites: 1

Brief Summary

The study proposes to investigate whether sleep deprivation will affect a variety of measures, including hormones, immune system functioning, and behaviors related to food intake and hunger. It is predicted that sleep deprivation will affect circulating neutrophil activity, and do so via affects on DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and down-regulate circulating oxytocin. Finally, it is predicted that sleep deprivation will increase participants' tendencies to pick larger portions of food, and also increase their tendency to purchase foods that are more caloric in a mock supermarket scenario.

Conditions

Sleep Deprivation; Sleep

Outcome Measures

Primary Outcomes (1)

• Neutrophil phagocytotic function production

  This project is designed to test the inflammatory capacity of circulating neutrophils. It also aims to investigate if any changes in neutrophil efficacy are governed by alterations in DNA methylation. Measured during 36 hours (at 1930 day 1, 0730 and 1930 day 2, 0730 day 3)

  Change in 4 neutrophil phagocytotic function from baseline to 36hours later (after the nighttime intervention)

Secondary Outcomes (3)

• Portion Size Task

  Change in selected portion size from baseline to 1 hr after consuming a caloric preload

• Neuro-economics task

  Change in purchasing behaviour 1 hr after consuming a caloric preload

• Circulating hormone levels

  Change in circulating hormone levels from baseline (ie. 1930 - before sleep intervention) to 12hr later (0730 after the nighttime intervention)

Study Arms (2)

Sleep deprivation

EXPERIMENTAL

Total sleep deprivation: participants were required to stay up for the entire night before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed

Behavioral: Neuroeconomics task; Behavioral: Portion Size Task; Procedure: Blood sample

Sleep

EXPERIMENTAL

Sleep: participants had an 8-h sleep opportunity before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed

Behavioral: Neuroeconomics task; Behavioral: Portion Size Task; Procedure: Blood sample

Interventions

Neuroeconomics task BEHAVIORAL

After a night of wakefulness, participants performed the neuroeconomics task (shopping food items in a mock supermarket scenario).

Sleep; Sleep deprivation

Portion Size Task BEHAVIORAL

Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume

Sleep; Sleep deprivation

Blood sample PROCEDURE

After a night of wakefulness, a blood sample was taken to assess the level and efficacy of circulating neutrophils, as well as to assay blood serum and plasma for the presence of hormones involved in hunger such as oxytocin and ghrelin

Sleep; Sleep deprivation

Eligibility Criteria

• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

• Male
• Age 18-35y
• Healthy (self-reported) and not on medication
• Non-smoking
• Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported)

You may not qualify if:

• Major illness
• Taking any serious medications
• Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
• Any dietary issues with the food items provided
• A history of endocrine or psychiatric disorders

Sponsors & Collaborators

• Uppsala University: lead

Study Sites (1)

Uppsala University

Uppsala, Uppsala County, 75105, Sweden

Location

Related Publications (2)

• Nilsson EK, Ernst B, Voisin S, Almen MS, Benedict C, Mwinyi J, Fredriksson R, Schultes B, Schioth HB. Roux-en Y gastric bypass surgery induces genome-wide promoter-specific changes in DNA methylation in whole blood of obese patients. PLoS One. 2015 Feb 24;10(2):e0115186. doi: 10.1371/journal.pone.0115186. eCollection 2015.

  PMID: 25710379 DERIVED

• Hogenkamp PS, Nilsson E, Nilsson VC, Chapman CD, Vogel H, Lundberg LS, Zarei S, Cedernaes J, Rangtell FH, Broman JE, Dickson SL, Brunstrom JM, Benedict C, Schioth HB. Acute sleep deprivation increases portion size and affects food choice in young men. Psychoneuroendocrinology. 2013 Sep;38(9):1668-74. doi: 10.1016/j.psyneuen.2013.01.012. Epub 2013 Feb 18.

  PMID: 23428257 DERIVED

MeSH Terms

Conditions

Sleep Deprivation

Interventions

Blood Specimen Collection

Condition Hierarchy (Ancestors)

Dyssomnias; Sleep Wake Disorders; Nervous System Diseases; Neurologic Manifestations; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Mental Disorders

Intervention Hierarchy (Ancestors)

Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Investigative Techniques

Study Officials

• Christian Benedict, PhD

  dept. of Neuroscience, Uppsala University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: October 11, 2012
• First Posted: November 21, 2012
• Study Start: February 1, 2012
• Primary Completion: March 1, 2013
• Study Completion: March 1, 2013
• Last Updated: November 21, 2012

Record last verified: 2012-11

Locations

SE (1)