Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage

NCT02082600 | Unknown DMC

• 1 other identifier: 2010070050
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (\~18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.

Conditions

Sleep; Sleep Deprivation

Outcome Measures

Primary Outcomes (1)

• Change from baseline in isometric strength

  Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage

Secondary Outcomes (1)

• Area Under Curve for serum total and free testosterone, IGF-1, and cortisol

  Blood samples will be collected every 2 hours, for 24 hours.

Other Outcomes (1)

• Area Under Curve for serum pro and anti-inflammatory cytokines

  Blood samples will be collected every 4 hours, for 24 hours.

Study Arms (2)

Sleep deprivation

EXPERIMENTAL

Exercise induced-muscle damage protocol followed by 60h of sleep deprivation (two nights) and one night of normal sleep (rebound).

Other: Sleep deprivation

Normal sleep

EXPERIMENTAL

Exercise induced-muscle damage protocol followed by 3 nights of normal sleep

Other: Normal sleep

Interventions

Sleep deprivation OTHER

This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and than will keep awake for 60 hours (2 nights), followed by 1 night of sleep, all inside the lab. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.

Also known as: Sleep and muscle recovery

Sleep deprivation

Normal sleep OTHER

This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and and will be able to sleep the 3 nights. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.

Normal sleep

Eligibility Criteria

• Age: 20 Years - 31 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy subjects
• sedentary
• sleep duration between 6-8 hours peer night

You may not qualify if:

• smokers
• use vitamin supplements, like C and E
• drink two doses of alcohol/day or \> 4 doses in one occasion
• use of anti-inflammatory drugs
• antilipidemic drugs
• anemia
• muscle or joint diseases
• cardiovascular diseases
• diabetes
• renal diseases
• sleep disturbances
• breathing disorders

Sponsors & Collaborators

• Federal University of São Paulo: lead
• Fundação de Amparo à Pesquisa do Estado de São Paulo: collaborator

Study Sites (1)

Associação Fundo de Incentivo à Pesquisa

São Paulo, São Paulo, 04020-050, Brazil

Location

MeSH Terms

Conditions

Sleep Deprivation

Interventions

Sleep

Condition Hierarchy (Ancestors)

Dyssomnias; Sleep Wake Disorders; Nervous System Diseases; Neurologic Manifestations; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Mental Disorders

Intervention Hierarchy (Ancestors)

Nervous System Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Marco Tulio Mello, PhD

  Federal University of São Paulo

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PhD

Study Record Dates

• First Submitted: March 6, 2014
• First Posted: March 10, 2014
• Study Start: July 1, 2013
• Primary Completion: December 1, 2013
• Last Updated: March 11, 2014

Record last verified: 2014-03

Locations

BR (1)