The Effect of Antioxidant Vitamin Supplementation on Muscle Performance and Redox Status After Eccentric Training

NCT01290458 | Completed DMC

• 1 other identifier: UTH-2008
• Study Type: interventional
• Target: 28
• Locations: 1 country
• Sites: 1

Brief Summary

Consumption of vitamin supplements is a common practice among athletes or people participating in health promoting exercise programs. The reason for this interest in vitamin supplements is primarily because of the observation that enhanced production of reactive oxygen and nitrogen species (RONS) influence fundamental biological processes, such as gene expression, signal transduction and enzyme activity. In a muscle and exercise physiology context, a low level of RONS is required for normal force production, whereas marked increases in RONS can cause contractile dysfunction, resulting in muscle weakness and fatigue. On the other hand RONS are involved in signaling pathways and serve to up-regulate the expression of a number of genes and can exert favorable effects such as training adaptations. The present study will employ a valid eccentric exercise model to examine the influence of combined vitamin C and E supplementation after acute and chronic eccentric exercise on muscle damage and performance, redox status, hemolysis and lipid and lipoprotein profile.

Conditions

Muscle Damage; Oxidative Stress; Hemolysis

Keywords

Eccentric training; Free radicals; Antioxidant supplements; Training adaptations; Lipid profile

Outcome Measures

Primary Outcomes (4)

• Maximum isometric torque (torque)

  An isokinetic dynamometer (Cybex, Ronkonkoma, NY) will be used for the measurement of isometric knee extensor peak torque at 90° knee flexion. The average of the three best maximal voluntary contractions with their dominant leg will be recorded. In order to ensure that the subjects provide their maximal effort, the measurements will be repeated if the difference between the lower and the higher torque value exceeded 10%. There will be two minutes rest between isometric efforts.

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

• Range of motion, ROM (degrees)

  The assessment of pain-free ROM will be performed manually using the the isokinetic dynamometer. The investigator will move the calf at a very low angular velocity from 0 knee extension to the position where the subject will feel any discomfort.

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

• Delayed onset muscle soreness, DOMS (scale 1-10)

  Each participant will assess delayed onset muscle soreness (DOMS) during squat movement (90o knee flexion) and perceived soreness will be rated on a scale ranging from 1 (normal) to 10 (very sore).

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

• Creatine kinase, CK (activity IU)

  CK activity will be measured as a general indicator of muscle damage.It will be measured in a Cobas Integra Plus 400 chemistry analyzer. CK will be measured before the beginning of the supplementation (week 0), before and the week after the first bout of eccentric exercise (week 5), before and the week after the second bout of eccentric exercise (week 11).

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

Secondary Outcomes (15)

• Reduced glutathione, GSH (μmol/g Hb)

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

• Oxidized glutathione, GSSG (μmol/g Hb)

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

• Thiobarbituric acid-reactive substances, TBARS (μM)

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

• Protein carbonyls (nmol/mg pr.)

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

• Catalase (μmol/min/mg Hb)

  Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)

Study Arms (2)

Vitamin

EXPERIMENTAL

Dietary Supplement: Antioxidant vitamins

Control

PLACEBO COMPARATOR

Dietary Supplement: Antioxidant vitamins

Interventions

Antioxidant vitamins DIETARY_SUPPLEMENT

Oral supplementation with one tablet of 1 g of vitamin C (Lamberts Healthcare Ltd, UK) and one tablet of 400 IU of vitamin E (Lamberts Healthcare Ltd, UK)

Also known as: For vitamin C ascorbic acid, For vitamin E d-alpha tocopherol

Control; Vitamin

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Physiological body mass index (BMI)
• Physiological health profile
• Subject provides written informed consent

You may not qualify if:

• Smoker
• Professional athlete
• Consumed any nutritional supplement the last 3 months
• Performed pure eccentric exercise the last 6 months
• Non Caucasian

Sponsors & Collaborators

• University of Thessaly: lead
• European University Cyprus: collaborator
• Greek Ministry of Development: collaborator
• Democritus University of Thrace: collaborator

Study Sites (1)

University of Thessaly

Trikala, 42100, Greece

Location

Related Publications (3)

• Paschalis V, Nikolaidis MG, Theodorou AA, Panayiotou G, Fatouros IG, Koutedakis Y, Jamurtas AZ. A weekly bout of eccentric exercise is sufficient to induce health-promoting effects. Med Sci Sports Exerc. 2011 Jan;43(1):64-73. doi: 10.1249/MSS.0b013e3181e91d90.

  PMID: 20508540 BACKGROUND

• Paschalis V, Nikolaidis MG, Giakas G, Theodorou AA, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Beneficial changes in energy expenditure and lipid profile after eccentric exercise in overweight and lean women. Scand J Med Sci Sports. 2010 Feb;20(1):e103-11. doi: 10.1111/j.1600-0838.2009.00920.x. Epub 2009 Apr 14.

  PMID: 19422638 BACKGROUND

• Nikolaidis MG, Paschalis V, Giakas G, Fatouros IG, Sakellariou GK, Theodorou AA, Koutedakis Y, Jamurtas AZ. Favorable and prolonged changes in blood lipid profile after muscle-damaging exercise. Med Sci Sports Exerc. 2008 Aug;40(8):1483-9. doi: 10.1249/MSS.0b013e31817356f2.

  PMID: 18614942 BACKGROUND

MeSH Terms

Conditions

Hemolysis

Interventions

Ascorbic Acid; alpha-Tocopherol

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Sugar Acids; Acids, Acyclic; Carboxylic Acids; Organic Chemicals; Hydroxy Acids; Carbohydrates; Tocopherols; Vitamin E; Benzopyrans; Pyrans; Heterocyclic Compounds, 1-Ring; Heterocyclic Compounds; Heterocyclic Compounds, 2-Ring; Heterocyclic Compounds, Fused-Ring

Study Officials

• Athanasios Z Jamurtas, Dr

  University of Thessaly

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Intervention Model: PARALLEL
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: February 4, 2011
• First Posted: February 7, 2011
• Study Start: June 1, 2009
• Primary Completion: November 1, 2009
• Study Completion: June 1, 2010
• Last Updated: February 7, 2011

Record last verified: 2011-01

Locations

GR (1)