Eccentric Exercise and Oxidative Stress

NCT01728675 | Completed

• 2 other identifiers: EUC-017EUC-019
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.

Conditions

Aging; Muscle Damage; Oxidative Stress

Keywords

Aging; Skeletal muscle; Biomarkers; Adaptation

Outcome Measures

Primary Outcomes (4)

• Muscle torque (torque)

  Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

• Range of motion, ROM (degrees)

  Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

• Delayed onset muscle soreness, DOMS (scale 1-10)

  Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

• Creatine kinase, CK (activity IU)

  Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Secondary Outcomes (10)

• F2-isoprostanes (pg/mg creatinine)

  Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

• Protein carbonyls (nmol/mg protein)

  Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

• Glutathione, GSH (μmol/g Hb)

  Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

• Catalase (U/g Hb)

  Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

• Superoxide dismutase, SOD (U/g Hb)

  Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Study Arms (2)

Young group

EXPERIMENTAL

Participants will underwent two isokinetic eccentric exercise sessions

Other: Exercise

Elderly group

EXPERIMENTAL

Participants will underwent two isokinetic eccentric exercise sessions

Other: Exercise

Interventions

Exercise OTHER

Participants will perform two isokinetic eccentric exercise bouts

Elderly group; Young group

Eligibility Criteria

• Age: 18 Years - 70 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• subject provides written informed consent
• physiological health profile
• stable at their anthropometric characteristics for at least the last year

You may not qualify if:

• muscle disease
• prior history of musculoskeletal injury to the lower limbs
• smokers
• consumed any nutritional supplement the last 3 months
• performed intense eccentric exercise the last 6 months
• non Caucasian

Sponsors & Collaborators

• European University Cyprus: lead
• Aristotle University Of Thessaloniki: collaborator

Study Sites (1)

European University Cyprus

Nicosia, 1516, Cyprus

Location

Related Publications (5)

• Nikolaidis MG, Kyparos A, Vrabas IS. F(2)-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res. 2011 Jan;50(1):89-103. doi: 10.1016/j.plipres.2010.10.002. Epub 2010 Oct 15.

  PMID: 20951733 BACKGROUND

• Nikolaidis MG, Jamurtas AZ. Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys. 2009 Oct 15;490(2):77-84. doi: 10.1016/j.abb.2009.08.015. Epub 2009 Aug 25.

  PMID: 19712664 BACKGROUND

• Nikolaidis MG, Kyparos A, Spanou C, Paschalis V, Theodorou AA, Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012 May 15;215(Pt 10):1615-25. doi: 10.1242/jeb.067470.

  PMID: 22539728 BACKGROUND

• Theodorou AA, Nikolaidis MG, Paschalis V, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. Med Sci Sports Exerc. 2010 Jun;42(6):1113-21. doi: 10.1249/MSS.0b013e3181c67ecd.

  PMID: 19997026 BACKGROUND

• Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31.

  PMID: 22288788 BACKGROUND

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Michalis G Nikolaidis, PhD

  Aristotle University Of Thessaloniki

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: SUPPORTIVE CARE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Researcher

Study Record Dates

• First Submitted: November 6, 2012
• First Posted: November 20, 2012
• Study Start: May 1, 2012
• Primary Completion: July 1, 2012
• Study Completion: July 1, 2012
• Last Updated: November 20, 2012

Record last verified: 2012-11

Locations

CY (1)