Recovery Kinetics Following Eccentric Exercise

NCT04733911 | Completed

• 1 other identifier: Recovery-Eccentrics-UTH
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study will be to investigate the recovery kinetics of neuromuscular fatigue, muscle microtrauma indicators, performance and oxidative stress factors after three different exercise induced muscle damage (EIMD) protocols in adult Males. Also, this study will determine the comparison among three different eccentric protocols in neuromuscular fatigue, muscle microtrauma indicators, performance and oxidative stress factors. The eccentric protocols will be differentiated in training volume and specifically in the eccentric repetitions in isokinetic dynamometer.

Conditions

Muscle Damage

Keywords

muscle damage; oxidative stress; Eccentric exercise; Neuromuscular fatigue; Recovery

Outcome Measures

Primary Outcomes (24)

• Change in white blood cell count

  White blood cell count will be measured using an automatic blood analyzer

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in catalase activity

  Concentration of catalase will be measured in red blood cells

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in uric acid in plasma

  concentration of uric acid will be measured in plasma

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in protein carbonyls in blood

  Concentration of protein carbonyls will be measured in red blood cells

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in total antioxidant capacity

  total antioxidant capacity will be measured in serum

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in reduced glutathione in blood

  Concentration of oxidized glutathione will be measured in red blood cells

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in thiobarbituric acid reactive substances

  Thiobarbituric acid will be measured in plasma

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in Creatine kinase in blood

  Creatine kinase will be measured in plasma

  At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in countermovement jump height

  Countermovement jump height will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height data for each leg.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in ground reaction force (GRF) during countermovement jump test

  The ground reaction force will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the GRF data for each leg.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in peak power during countermovement jump test

  The peak power will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the peak power data for each leg.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in mean power during countermovement jump test

  The mean power will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the mean power data for each leg.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in vertical stifness during countermovement jump test

  The vertical stiffness will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of vertical stifness data for each leg.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in peak rate of force develpemnt during countermovement jump test

  The Peak rate of force development will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the Peak RFD data for each leg.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in peak normalized EMG during the eccentric and concentric phases of the counter movement jump

  Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system (Myon AG, Schwarzenberg, Switzerland) for the vastus medialis (VM) and vastus lateralis muscles.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in mean normalized EMG during the eccentric and concentric phases of the counter movement jump

  Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system (Myon AG, Schwarzenberg, Switzerland) for the vastus medialis (VM) and vastus lateralis muscles.

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in knee range of motion

  Knee range of motion will be measured by goniometer

  At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in eccentric isokinetic knee extensors peak torque

  Eccentric isokinetic knee extensors peak torque will be assessed on an isokinetic dynamometer

  At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in concentric isokinetic knee extensors peak torque

  Concentric isokinetic knee extensors peak torque will be assessed on an isokinetic dynamometer

  At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in eccentric isokinetic knee flexors peak torque

  Eccentric isokinetic knee flexors peak torque will be assessed on an isokinetic dynamometer

  At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in concentric isokinetic knee flexors peak torque

  Concentric Iisokinetic knee flexors peak torque will be assessed on an isokinetic dynamometer

  At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in isometric peak torque

  The isometric peak torque will be assessed on an isokinetic dyanmometer

  At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in fatigue index of isometric torque during 10 second

  Fatigue rate during MVIC will be estimated through the percent drop of peak torque between the first and the last three seconds of a 10-secong maximal isometric contraction.

  At baseline, 1hour, at 24, 48 and 192 hours following the eccentric exercise protocol.

• Change in delayed onset of muscle soreness (DOMS) in the knee flexors (KF) and extensors (KE) of both limbs

  Muscle soreness (KF and KE) will be assessed during palpation of the muscle belly and the distal region

  At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.

Secondary Outcomes (5)

• Body weight

  At baseline

• Body height

  At baseline

• Body fat

  At baseline

• Dietary intake

  Over a 5 -day period at baseline

• Maximal oxygen consumption (VO2max)

  At baseline

Study Arms (4)

Eccentric 75

EXPERIMENTAL

Participants in this arm will perform an eccentric exercise protocol consisted of 75 eccentric repetetions on an isokinetic dynamometer.

Other: 75 eccentric contractions

Eccentric 150

EXPERIMENTAL

Participants in this arm will perform an eccentric exercise protocol consisted of 150 eccentric repetetions on an isokinetic dynamometer.

Other: 150 eccentric contractions

Eccentric 300

EXPERIMENTAL

Participants in this arm will perform an eccentric exercise protocol consisted of 300 eccentric repetetions on an isokinetic dynamometer.

Other: 300 eccentric contractions

Control

NO INTERVENTION

Participants in this arm will receive no intervention.

Interventions

75 eccentric contractions OTHER

An acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 5 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec.

Eccentric 75

150 eccentric contractions OTHER

An acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 10 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec.

Eccentric 150

300 eccentric contractions OTHER

An acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 20 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec.

Eccentric 300

Eligibility Criteria

• Age: 18 Years - 30 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Aged between 18 and 30 years
• Body Mass Index between 18.5-24.9 kg/m\*m
• Free of chronic diseases
• Free of musculoskeletal injury
• participants should be non-smokers

You may not qualify if:

• Musculoskeletal injury
• Chronic disease
• Use of alcohol, caffeine and any type of nutritional supplements or medication before (≥ 6 months) and throughout the study.

Sponsors & Collaborators

• University of Thessaly: lead

Study Sites (1)

University o Thessaly, School of Physical Education and Sports Science

Trikala, 42100, Greece

Location

Study Officials

• Ioannis G Fatouros, Prof

  University of Thessaly

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: January 25, 2021
• First Posted: February 2, 2021
• Study Start: April 1, 2021
• Primary Completion: February 28, 2022
• Study Completion: April 30, 2022
• Last Updated: June 10, 2022

Record last verified: 2022-06

Locations

GR (1)