Acute Endocrine Responses to Aerobic and Anaerobic Exercise in Sedentary Women: A Comparative Analysis of Thyroid Hormones and Cortisol

NCT07823335 | Recruiting

• 1 other identifier: KBU-THYROID-2026-01
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to compare acute thyroid hormone and cortisol responses to aerobic and anaerobic exercise in sedentary women. Thirty sedentary women aged 18-25 years will voluntarily participate in the study. Participants will complete a 20-meter shuttle run test as the aerobic exercise protocol and a 30-second Wingate test as the anaerobic exercise protocol in separate testing sessions. Blood samples will be collected immediately before and after each exercise protocol. Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations will be determined using chemiluminescence-based laboratory methods. Changes in hormone concentrations will be compared between the aerobic and anaerobic exercise conditions.

Conditions

Sedentary Behavior; Acute Exercise; Endocrine Response

Keywords

Aerobic Exercise; Anaerobic Exercise; Sedentary Women; Cortisol; Thyroid Hormones

Outcome Measures

Primary Outcomes (1)

• Change in Serum Cortisol Concentration

  Immediately before and immediately after each exercise protocol

Study Arms (1)

Aerobic and Anaerobic Exercise

EXPERIMENTAL

Participants will complete both a progressive 20-meter shuttle run test and a 30-second Wingate anaerobic test in separate testing sessions. Venous blood samples will be collected immediately before and after each exercise protocol to assess acute changes in serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations. Each participant will serve as her own control.

Behavioral: 20-Meter Shuttle Run Test; Behavioral: Wingate Anaerobic Test

Interventions

20-Meter Shuttle Run Test BEHAVIORAL

Participants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. The initial running speed will progressively increase as the interval between audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions.

Aerobic and Anaerobic Exercise

Wingate Anaerobic Test BEHAVIORAL

Participants will perform a 30-second Wingate anaerobic test on a Monark cycle ergometer connected to computerized analysis software. Following an unloaded acceleration phase of approximately 3-4 seconds, a resistance corresponding to 65 g/kg of body mass will be applied. Participants will be instructed to pedal against this resistance at maximal effort for 30 seconds. Peak power, mean power, minimum power, and fatigue index will be recorded.

Aerobic and Anaerobic Exercise

Eligibility Criteria

• Age: 18 Years - 24 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Female participants aged 18-25 years
• Studying at the Faculty of Sports Sciences of Yalova University
• Not actively participating in regular exercise or sports training
• Voluntarily agreeing to participate in the study
• Providing written informed consent
• Being medically eligible to perform the exercise protocols following preparticipation medical screening

You may not qualify if:

• Having a neurological disorder during the previous year
• Having an auditory, visual, or vestibular disorder during the previous year
• Having experienced a serious upper- or lower-extremity injury during the previous six months
• Having any medical condition identified during screening that could prevent safe participation in maximal exercise testing
• Being unable or unwilling to complete either exercise protocol or blood-sampling procedures

Sponsors & Collaborators

• Assoc. Prof. Dr. Özgür EKEN: lead
• Karabuk University: collaborator

Study Sites (1)

Karabuk University, Hasan Dogan Faculty of Sport Sciences

Karabük, Karabük Province, 78050, Turkey (Türkiye)

RECRUITING

MeSH Terms

Conditions

Sedentary Behavior

Condition Hierarchy (Ancestors)

Behavior

Central Study Contacts

Özgür EKEN, PhD

CONTACT

+905532132795; ozgur.eken@inonu.edu.tr

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Assoc. Prof.Dr.

Model Details: Each participant will complete both the aerobic and anaerobic exercise interventions in two separate testing sessions and will serve as her own control. Acute changes in thyroid hormone and cortisol concentrations from before to after each exercise protocol will be compared between the two exercise conditions.

Study Record Dates

• First Submitted: September 11, 2026
• First Posted: September 16, 2026
• Study Start: September 15, 2026
• Primary Completion: September 30, 2026
• Study Completion: September 30, 2026
• Last Updated: September 16, 2026

Record last verified: 2026-09

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)