Effects of Aerobic and Resistance Exercise on Cardiovascular Biomarkers in Sedentary Women

NCT07457047 | Completed

• 1 other identifier: TR.ANKAU.REC.E-33665751
• Study Type: interventional
• Target: 45
• Locations: 1 country
• Sites: 1

Brief Summary

This randomized controlled trial investigated the effects of aerobic and resistance exercise on cardiovascular and inflammatory biomarkers in sedentary young women. Forty-five healthy sedentary females aged 18 to 25 years were randomly assigned to aerobic exercise, resistance exercise, or control groups. The exercise groups trained three times per week for 8 weeks under supervision, while the control group maintained a sedentary lifestyle. Blood samples and physiological measurements were obtained at baseline and after the intervention. The primary aim was to evaluate changes in circulating natriuretic peptides and inflammatory biomarkers, including NT-proBNP, BNP, soluble ST2, and Galectin-3. Secondary outcomes included lipid profile, glucose and insulin levels, blood pressure, and aerobic fitness. The study examined whether different exercise modalities induce beneficial cardiometabolic adaptations in previously sedentary young women.

Conditions

Sedentary Lifestyle; Cardiometabolic Risk

Keywords

Exercise; Aerobic Exercise; Resistance Training; NT-proBNP; BNP; Galectin-3; sST2; Cardiometabolic Biomarkers; Sedentary Women

Outcome Measures

Primary Outcomes (1)

• Change in Serum NT-proBNP Level

  Difference in fasting serum N-terminal pro-brain natriuretic peptide (NT-proBNP) concentration between baseline and after 8 weeks of intervention.

  Baseline and Week 8

Study Arms (3)

Aerobic Exercise

EXPERIMENTAL

Participants performed supervised moderate-intensity aerobic exercise on a treadmill (walking and jogging) at 60-75% of heart rate reserve, 60 minutes per session, three times per week for 8 weeks.

Behavioral: Aerobic Exercise Training

Resistance Exercise

EXPERIMENTAL

Participants performed supervised whole-body resistance training consisting of major upper and lower body exercises, 3 sets of 8-10 repetitions at approximately 70-80% of one-repetition maximum, three times per week for 8 weeks.

Behavioral: Resistance Exercise Training

Resistance Training

NO INTERVENTION

Participants maintained their habitual sedentary lifestyle and did not participate in any structured exercise program for 8 weeks.

Interventions

Aerobic Exercise Training BEHAVIORAL

Supervised treadmill-based moderate-intensity walking and jogging at 60-75% of heart rate reserve, 60 minutes per session including warm-up and cool-down, three sessions per week for 8 weeks.

Aerobic Exercise

Resistance Exercise Training BEHAVIORAL

Supervised whole-body resistance training including major muscle group exercises, performed as 3 sets of 8-10 repetitions at approximately 70-80% of one-repetition maximum, three sessions per week for 8 weeks.

Resistance Exercise

Eligibility Criteria

• Age: 18 Years - 25 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Female, aged 18 to 25 years
• Sedentary lifestyle (no regular structured exercise training)
• Apparently healthy with no diagnosed cardiovascular, metabolic, or inflammatory disease
• Willing to participate in an 8-week supervised exercise program and provide blood samples
• Provided written informed consent

You may not qualify if:

• History of cardiovascular disease, diabetes mellitus, or chronic inflammatory disorder
• Current use of medications affecting cardiovascular or metabolic function
• Regular participation in structured exercise or competitive sports
• Smoking or alcohol/drug abuse
• Any musculoskeletal or medical condition that prevents safe participation in exercise
• Pregnancy or suspected pregnancy

Sponsors & Collaborators

• Ondokuz Mayıs University: lead

Study Sites (1)

Ankara University

Ankara, Besevler, 06100, Turkey (Türkiye)

Location

Related Publications (3)

• Schmitt VH, Prochaska JH, Foll AS, Schulz A, Keller K, Hahad O, Koeck T, Trobs SO, Rapp S, Beutel M, Pfeiffer N, Strauch K, Lackner KJ, Munzel T, Wild PS. Galectin-3 for prediction of cardiac function compared to NT-proBNP in individuals with prediabetes and type 2 diabetes mellitus. Sci Rep. 2021 Sep 24;11(1):19012. doi: 10.1038/s41598-021-98227-x.

  PMID: 34561496 BACKGROUND

• Lavie CJ, Ozemek C, Carbone S, Katzmarzyk PT, Blair SN. Sedentary Behavior, Exercise, and Cardiovascular Health. Circ Res. 2019 Mar;124(5):799-815. doi: 10.1161/CIRCRESAHA.118.312669.

  PMID: 30817262 BACKGROUND

• Rukavina Mikusic NL, Kouyoumdzian NM, Puyo AM, Fernandez BE, Choi MR. Role of natriuretic peptides in the cardiovascular-adipose communication: a tale of two organs. Pflugers Arch. 2022 Jan;474(1):5-19. doi: 10.1007/s00424-021-02596-1. Epub 2021 Jun 26.

  PMID: 34173888 BACKGROUND

MeSH Terms

Conditions

Sedentary Behavior; Motor Activity

Interventions

Resistance Training

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor of Sports Sciences

Model Details: Participants were randomly assigned to one of three parallel groups: aerobic exercise, resistance exercise, or non-exercising control, and followed for 8 weeks.

Study Record Dates

• First Submitted: January 31, 2026
• First Posted: March 9, 2026
• Study Start: January 22, 2023
• Primary Completion: November 23, 2023
• Study Completion: May 1, 2025
• Last Updated: March 9, 2026

Record last verified: 2026-03

Data Sharing

• IPD Sharing: Will share

Locations

TU (1)