Blood Parameter Changes Following Aerobic Exercise in Professional Soccer Players

NCT07826598 | Not Yet Recruiting

• 1 other identifier: KBÜ-AEROBIC-BLOOD-2026-01
• Study Type: interventional
• Target: 40
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to investigate acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) levels following aerobic exercise in professional soccer players. Twenty professional male soccer players will voluntarily participate in the study. Participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and after the exercise protocol. Pre- to post-exercise changes in each blood parameter will be analyzed to determine the acute physiological responses to aerobic exercise in professional soccer players.

Conditions

Exercise Physiology; Blood Parameters

Keywords

Aerobic Exercise; Professional Soccer Players; Blood Parameters; Exercise Physiology; Acute Exercise

Outcome Measures

Primary Outcomes (4)

• Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise

  Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum ferritin concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

  Immediately before exercise and immediately after completion of the 20-meter shuttle run test

• Change in Serum Folate Concentration From Pre-Exercise to Immediately Post-Exercise

  Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum folate concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

  Immediately before exercise and immediately after completion of the 20-meter shuttle run test

• Change in Serum Vitamin B12 Concentration From Pre-Exercise to Immediately Post-Exercise

  Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum vitamin B12 concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

  Immediately before exercise and immediately after completion of the 20-meter shuttle run test

• Change in Serum Parathyroid Hormone Concentration From Pre-Exercise to Immediately Post-Exercise

  Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum parathyroid hormone (PTH) concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

  Immediately before exercise and immediately after completion of the 20-meter shuttle run test

Study Arms (1)

Aerobic Exercise

EXPERIMENTAL

All participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and immediately after the exercise protocol to assess acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone concentrations.

Behavioral: 20-Meter Shuttle Run Test

Interventions

20-Meter Shuttle Run Test BEHAVIORAL

Participants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions.

Aerobic Exercise

Eligibility Criteria

• Age: 18 Years - 25 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Willing and able to participate in the study procedures.
• Provision of informed consent.

You may not qualify if:

• \- Withdrawal from the study or unwillingness to continue participation.

Sponsors & Collaborators

• Inonu University: lead

Study Sites (1)

Exercise Physiology Laboratory, Faculty of Sport Sciences, Karabuk University

Karabük, Turkey (Türkiye)

Location

Central Study Contacts

Özgür EKEN, PhD

CONTACT

+905532132795; ozgur.eken@inonu.edu.tr

musa şahin, PhD

CONTACT

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Masking Details: No masking was applied in this single-group exercise intervention study.
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assoc. Prof.Dr.

Model Details: All participants will be assigned to a single intervention group and will complete the same aerobic exercise protocol. Blood parameters will be assessed in relation to the exercise intervention using a within-participant design.

Study Record Dates

• First Submitted: August 29, 2026
• First Posted: September 17, 2026
• Study Start: August 29, 2026
• Primary Completion: September 29, 2026
• Study Completion: September 29, 2026
• Last Updated: September 17, 2026

Record last verified: 2026-09

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)