Assessments of Metabolic Responses to Acute Oral Administration of Sucrose, Glucose, and Fructose

NCT06799715 | Active Not Recruiting DMC

• 1 other identifier: SuGluFru
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

This project aims to compare the acute metabolic effects of the three sweeteners sucrose, glucose, and fructose on GI hormones (GLP-1, PYY, CCK, and ghrelin). Furthermore, glycemic control, erythritol and xylitol concentrations, blood coagulation function , blood lipids, uric acid, high-sensitive C-reactive protein (hsCRP), complete blood count, gastric emptying, appetite-related sensations, and GI symptoms will be investigated.

Conditions

Gastrointestinal Hormones; Glycemic Control; Blood Coagulation Function; Gastric Emptying

Outcome Measures

Primary Outcomes (4)

• Effect of sucrose, glucose, fructose, and water on the GI hormone response - GLP-1

  Plasma GLP-1 will be measured with a commercially available immunoassay kit (MILLIPLEX® MAP; Millipore Corporation, Billerica, MA, USA).

  Blood will be drawn at the following timepoints: : -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration)

• Effect of sucrose, glucose, fructose, and water on the GI hormone response - PYY

  PYY-3-36 will be quantified using a non-radioactive high sensitive sandwich ELISA (Millipore - # EZHPYYT66K).

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

• Effect of sucrose, glucose, fructose, and water on the GI hormone response - CCK

  Plasma cholecystokinin (CCK) levels will be measured with a sensitive radioimmunoassay using a highly specific antiserum (No. 92128)

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

• Effect of sucrose, glucose, fructose, and water on the GI hormone response - Ghrelin

  Octanoylated ghrelin will be measured by a radioimmunoassay with 125I \[Tyr24\] human ghrelin as tracer and a rabbit antibody against human ghrelin (final dilution 1/100000), which does not cross-react with desoctanoylated ghrelin.

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

Secondary Outcomes (16)

• Effects on glycemic control - plasma glucose

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

• Effects on glycemic control - plasma insulin

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

• Effects on glycemic control - plasma c-peptide

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

• Effects on glycemic control - plasma glucagon

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

• Effects on erythritol concentrations

  Blood will be drawn at the following time points: -10 and -1 minutes (before administration) and 15, 30, 45, 60, 90, 120, and 180minutes (after administration).

Study Arms (4)

Sucrose

ACTIVE COMPARATOR

20 volunteers receive 33.5g sucrose dissolved in 300mL water as a drink.

Dietary Supplement: Sucrose

Glucose

ACTIVE COMPARATOR

20 volunteers receive 33.5g glucose dissolved in 300mL water as a drink.

Dietary Supplement: Glucose

Fructose

ACTIVE COMPARATOR

20 volunteers receive 33.5g fructose dissolved in 300mL water as a drink.

Dietary Supplement: Fructose

Water

PLACEBO COMPARATOR

20 volunteers receive 300mL water as a drink.

Dietary Supplement: Water

Interventions

Sucrose DIETARY_SUPPLEMENT

33.5g sucrose dissolved in 300mL tap water

Also known as: Saccharose

Sucrose

Glucose DIETARY_SUPPLEMENT

33.5g glucose dissolved in 300mL tap water

Also known as: Dextrose

Glucose

Fructose DIETARY_SUPPLEMENT

33.5g fructose dissolved in 300mL tap water

Fructose

Water DIETARY_SUPPLEMENT

300mL water

Water

Eligibility Criteria

• Age: 18 Years - 55 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy, non-diabetic (glycated hemoglobin (HbA1c) \< 5.7%, fasting glucose \< 5.6 mmol/L), and normal weight participants with a body-mass index (BMI) of 19.0-24.9 kg/m2, parameters of complete blood count within normal range
• Age 18-55 years
• Stable body weight (± 5%) for at least three months
• Able to give informed consent as documented by signature

You may not qualify if:

• Fructose intolerance
• Any pre-existing diet (e.g., vegetarian diet, vegan diet, sugar free diet, paleo diet, Atkins diet, ketogenic diet) that deviates from normal eating habits
• Regular consumption (\>1/ week) of erythritol or xylitol
• Regular intake of medications, except contraceptives
• Pre-existing impairment of blood coagulation/thrombocyte function (e.g. hereditary, regular intake of anti-coagulant agents (e.g. NSAIDs, heparin, warfarin, etc.))Chronic or clinically relevant acute infections/diseases
• Substance abuse (more than 1 glass wine/beer per day; regular consumption of cannabis, consumption of cocaine, heroin, etc.), regular smoking
• Pregnancy: although no contraindication, pregnancy might influence metabolic state. Women who are pregnant or have the intention to become pregnant during the course of the study are excluded. In female participants a urine pregnancy test is carried out upon screening.
• Shift worker
• Participation in another study with investigational drug within the 30 days preceding and during the present study

Sponsors & Collaborators

• University Hospital, Basel, Switzerland: lead

Study Sites (1)

St. Claraspital

Basel, 4002, Switzerland

Location

MeSH Terms

Interventions

Sucrose; Glucose; Fructose; Water

Intervention Hierarchy (Ancestors)

Disaccharides; Oligosaccharides; Polysaccharides; Carbohydrates; Sugars; Hexoses; Monosaccharides; Ketoses; Hydroxides; Alkalies; Inorganic Chemicals; Anions; Ions; Electrolytes; Oxides; Oxygen Compounds

Study Officials

• Anne Christin Meyer-Gerspach, PD, PhD

  St. Clara Research Ltd.

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: January 23, 2025
• First Posted: January 29, 2025
• Study Start: February 6, 2025
• Primary Completion (Estimated): September 1, 2026
• Study Completion (Estimated): September 1, 2026
• Last Updated: May 1, 2026

Record last verified: 2026-04

Data Sharing

• IPD Sharing: Will not share

Locations

CH (1)