Effects of Exercise on Fructose Metabolism

NCT01866215 | Completed

• 1 other identifier: University of Lausanne
• Study Type: interventional
• Target: 8
• Locations: 1 country
• Sites: 1

Brief Summary

A high fructose diet increases fasting and post-prandial triglyceride (TG) concentrations in sedentary healthy human subjects.These effects may be secondary to fructose-induced hepatic de novo lipogenesis. Recent evidence indicate that exercise can prevent fructose induced dyslipidemia.This study will evaluate

1. 1.how exercise effects the metabolic fate of oral fructose 1a) when exercise is performed before fructose ingestion 1b) when exercise is performed after fructose ingestion Metabolic effects of exercise will be assessed in healthy male subjects by measuring fructose oxidation (13CO2 production), fructose conversion into glucose (13C glucose concentrations in blood) and hepatic fructose conversion into lipid (13C palmitate-very low density lipoprotein (VLDL) concentrations in blood) after ingestion of 13C-labelled fructose meals
2. 2.how fructose and protein modulate muscle glycogen and intramyocellular lipid repletion after exercise Healthy male subjects will be fed various fructose, glucose, lipid and whey protein meals after a glycogen/intramyocellular lipid depleting exercise. The effects of meals' composition will be assessed after 24 hours by measuring intramyocellular lipids and glycogen using proton-magnetic resonance spectroscopy (MRS).

Conditions

Healthy Subjects

Keywords

gluconeogenesis; de novo lipogenesis; intramyocellular lipids; muscle glycogen; fructose

Outcome Measures

Primary Outcomes (3)

• Plasma triglyceride

  measurement of total and VLDL-TG concentrations in study 1

  measurements in fasting conditions and every hour from 7:00 am to 4:00 pm (up to 9 hours)

• intramyocellular lipids

  Measurement by 1H-MRS in muscle vastus lateralis in study 2

  after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise

• Fructose conversion into lipids

  Estimated from 13C palmitate-VLDL concentration

  measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)

Secondary Outcomes (4)

• Fructose conversion into glucose

  measured between 7:00 am and 4:oo pm (up to 9 hours)

• intramyocellular glycogen concentrations

  after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise

• fructose oxidation

  measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)

• exogenous lipid oxidation

  measured from 7:00 am to 4:00 pm in study 2 (up to 9 hours)

Study Arms (5)

Study1a

EXPERIMENTAL

exercice performed 90 min before 13C fructose meal ingestion

Other: exercise

study 1b

EXPERIMENTAL

exercise performed 90 min after 13C fructose meal ingestion

Other: exercise

study 1c

NO INTERVENTION

no exercise

study 2a

EXPERIMENTAL

meals containing fructose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise

Dietary Supplement: fructose

study 2b

ACTIVE COMPARATOR

meals containing glucose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise

Dietary Supplement: glucose

Interventions

exercise OTHER

cycling at 100W during 60 min

Study1a; study 1b

fructose DIETARY_SUPPLEMENT

isocaloric nutrition with fructose, cream and whey protein during the 24 hour following a glycogen/intramyocellular lipid depleting exercise

study 2a

glucose DIETARY_SUPPLEMENT

isocaloric nutrition with glucose, cream and whey protein during the 24 hour following a glycogen/intramyocellular lipid depleting exercise

study 2b

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• males
• years old
• kg/m2\>BMI\>25kg/m2
• moderate to high usual physical activity
• non-smokers

You may not qualify if:

• family history of diabetes mellitus
• ECG anomaly
• any medication
• participation to blood spending or other clinical study in the 3 months before the beginning of this study
• consumption of drugs
• consumption of more than 50g alcool/week
• family history of food intolerance

Sponsors & Collaborators

• University of Lausanne: lead
• University of Bern: collaborator
• Centre Hospitalier Universitaire Vaudois: collaborator

Study Sites (1)

Centre Hospitalier Universitaire Vaudois

Lausanne, Canton of Vaud, CH-1011, Switzerland

Location

Related Publications (2)

• Rosset R, Lecoultre V, Egli L, Cros J, Dokumaci AS, Zwygart K, Boesch C, Kreis R, Schneiter P, Tappy L. Postexercise repletion of muscle energy stores with fructose or glucose in mixed meals. Am J Clin Nutr. 2017 Mar;105(3):609-617. doi: 10.3945/ajcn.116.138214. Epub 2017 Jan 18.

  PMID: 28100512 DERIVED

• Egli L, Lecoultre V, Cros J, Rosset R, Marques AS, Schneiter P, Hodson L, Gabert L, Laville M, Tappy L. Exercise performed immediately after fructose ingestion enhances fructose oxidation and suppresses fructose storage. Am J Clin Nutr. 2016 Feb;103(2):348-55. doi: 10.3945/ajcn.115.116988. Epub 2015 Dec 23.

  PMID: 26702120 DERIVED

MeSH Terms

Interventions

Exercise; Fructose; Glucose

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Hexoses; Monosaccharides; Sugars; Carbohydrates; Ketoses

Study Officials

• Luc Tappy, MD

  University of Lausanne

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor of Physiology

Study Record Dates

• First Submitted: May 27, 2013
• First Posted: May 31, 2013
• Study Start: May 1, 2013
• Primary Completion: January 1, 2014
• Study Completion: January 1, 2014
• Last Updated: June 4, 2014

Record last verified: 2014-06

Locations

CH (1)