Sleep Restriction and Postprandial Lipemia

NCT05713370 | Completed

• 1 other identifier: 2090503
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 2

Brief Summary

Sleep restriction increases overnight and early morning non-esterified fatty acids (NEFA) levels, which are correlated with whole-body decreases in insulin sensitivity, consistent with the observed impairment of intracellular insulin signaling. Adipose tissue biopsies from sleep restricted subjects that are insulin stimulated have reduced phosphorylation of protein kinase B (pAKT). This protein is involved in suppression of intracellular lipolysis and NEFA release. Aerobic exercise has beneficial effects on postprandial lipemia and insulinemia in normal-weight and obese individuals. Acute moderate-intensity aerobic exercise (30-90 min) performed 12-18 h before an oral fat tolerance test or mixed meal test reduces postprandial triglycerides (TG) and insulin concentrations. This response is largely dependent upon the exercise-induced energy deficit as the response is abolished when the calories expended during exercise are replaced. However, it is not known if sleep restriction will interfere with the beneficial effects of prior exercise on postprandial lipemia. The aim of this project is to investigate if sleep restriction negates the positive effect that exercise has on postprandial lipemia. It is hypothesized that sleep restriction will negate the beneficial effects of prior exercise on postprandial lipemia. Additionally sleep restriction will result in a worsening of the lipid profile compared to no exercise. For the proposed study, the investigators will use a repeated measures analysis of variance (ANOVA) (4 study conditions (no exercise+ sleep restriction, no exercise+normal sleep, exercise+normal sleep, exercise+sleep restriction) x time will be used to analyze changes in NEFA and TG concentrations while a one way ANOVA will be used to analyze area under the curve of the NEFA and TG concentrations.

Conditions

Sleep Deprivation; Obesity

Outcome Measures

Primary Outcomes (2)

• area under the curve of fatty acids concentrations

  blood samples for free fatty acid concentrations will be taken every 30 minutes for 4 hours

  4 hour

• area under the curve of triglycerides concentrations

  blood samples for triglyceride concentrations will be taken every 30 minutes for 4 hours

  4 hour

Secondary Outcomes (1)

• area under the curve of glucose concentrations

  4 hour

Study Arms (4)

no exercise, no SR

PLACEBO COMPARATOR

75 g of glucose will be given at the beginning of the study day (the evening prior there will be no exercise the night before the study day, normal sleep (8 h))

Dietary Supplement: high fat meal

no exercise, SR

EXPERIMENTAL

75 g of glucose will be given at the beginning of the study day (the evening prior there will be no exercise the night before the study day, 4 h of sleep the previous night)

Dietary Supplement: high fat meal

Exercise, no SR

EXPERIMENTAL

75 g of glucose will be given at the beginning of the study day (the evening prior there will be 45 min of exercise the night before the study day, normal sleep (8 h))

Dietary Supplement: high fat meal

Exercise, SR

EXPERIMENTAL

75 g of glucose will be given at the beginning of the study day (the evening prior there will be 45 min of exercise the night before the study day, 4 h of sleep the previous night)

Dietary Supplement: high fat meal

Interventions

high fat meal DIETARY_SUPPLEMENT

A high fat meal (milkshake) will be administered on the morning after the intervention of no exercise and no SR the night before.

Exercise, SR; Exercise, no SR; no exercise, SR; no exercise, no SR

Eligibility Criteria

• Age: 21 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Overweight and obese men and women
• years of age
• BMI of 25-35 kg/m2
• Normal sleeping habits of 7-9 hours per night

You may not qualify if:

• type 2 diabetic
• diagnosed with cardiovascular disease
• hypertensive
• smokers
• pregnant
• taking lipid-lowering medications
• sleep apnea
• fragmented sleep
• have any recent changes in hormonal birth control
• night shift workers or take regular daytime naps
• any medications known to impact metabolism, appetite, or sleep
• any allergies to milk, ice cream, peanut butter and soy.

Sponsors & Collaborators

• University of Missouri-Columbia: lead

Study Sites (2)

University of Misouri

Columbia, Missouri, 65211, United States

Location

University of Missouri

Columbia, Missouri, 65211, United States

Location

Related Publications (1)

• Maloney A, McDonald M, Petroski G, Kanaley JA. Effect of sleep restriction, with or without prior evening exercise, on morning postprandial lipemia. Appl Physiol Nutr Metab. 2025 Jan 1;50:1-10. doi: 10.1139/apnm-2024-0535.

  PMID: 40294439 DERIVED

MeSH Terms

Conditions

Sleep Deprivation; Obesity

Condition Hierarchy (Ancestors)

Dyssomnias; Sleep Wake Disorders; Nervous System Diseases; Neurologic Manifestations; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Mental Disorders; Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight

Study Officials

• Jill Kanaley, PhD

  University of Missouri-Columbia

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: professor

Model Details: Subjects will complete all arms of the study in a counterbalanced design

Study Record Dates

• First Submitted: January 13, 2023
• First Posted: February 6, 2023
• Study Start: July 1, 2022
• Primary Completion: December 1, 2023
• Study Completion: December 1, 2023
• Last Updated: January 31, 2024

Record last verified: 2024-01

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP
• Time Frame: The data will not be shared until at least 2 years after data collection is completed. Data will be available for another 3 years

Locations

US (2)