Recovery From 50 Eccentric Biceps Curls in Young, Untrained Men and Women

NCT05036239 | Completed

• 1 other identifier: EIMD19
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of the study is to investigate muscle stiffness in relation to muscle damaging work and to investigate how well the change in muscle stiffness correlates with the degree of muscle damage (myofibrillar disruption and necrosis). To date, the reduction in force-generating capacity is the best non-invasive marker of muscle damage. It is already established that muscle stiffness correlates well with the decline in force-generating capacity after damaging exercise. However, the correlation between degree of muscle damage and muscle stiffness has not yet been investigated. The main focus of the study is therefore to investigate the relationship between muscle stiffness and muscle damage. Further, the researchers aim to investigate how calcium cycling is affected by damaging work, and if impaired calcium cycling may partially explain the observed reduction in force-generating capacity.

Conditions

Recovery; Muscle Damage; Muscle Stiffness; Cell Structure Alteration; Calcium Cycling

Outcome Measures

Primary Outcomes (4)

• Change in muscle strength

  Recovery of arm flexion torque

  Baseline, and 5 minutes, 3 hours, 24 hours, 48 hours, 72 hours, and 96 hours after eccentric biceps curls

• Change in muscle stiffness

  Muscle stiffness measured with shear wave elastography as mean young modulus in different conditions (static and dynamic)

  Baseline, and 50 minutes, 3 hours, 24 hours, 48 hours, 72 hours, and 96 hours after eccentric biceps curls

• Change in muscle damage

  Development of myofibrillar disruption and necrosis observed in skeletal muscle biopsies with electron and confocal microscopy

  2 hours, 48 hours, and 96 hours after eccentric biceps curls

• Change in calcium cycling

  Calcium cycling in muscle single fibers and Sarcoplasmic reticulum-homogenate

  2 hours, 48 hours and 96 hours after eccentric biceps curls

Secondary Outcomes (1)

• Change in organization of the tubular system in skeletal muscle

  2 hours, 48 hours and 96 hours after eccentric biceps curls

Other Outcomes (17)

• Change in HSP70

  2 hours, 48 hours and 96 hours after eccentric biceps curls

• Change in AlphaB-crystallin

  2 hours, 48 hours and 96 hours after eccentric biceps curls

• Change in Fiber-specific AlphaB-crystallin staining intensity

  2 hours, 48 hours and 96 hours after eccentric biceps curls

Study Arms (2)

Exercised

EXPERIMENTAL

One bout of 50 eccentric biceps curls

Other: Eccentric biceps curls

Control

NO INTERVENTION

No eccentric biceps curls

Interventions

Eccentric biceps curls OTHER

10 x 5 repetitions of eccentric biceps curls, interspaced by 30 seconds of rest.

Exercised

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• \- 18 to 35 years of age

You may not qualify if:

• Injury to the muscle-skeletal system
• Other conditions causing inability to perform heavy-load resistance exercise
• Having engaged in resistance exercise targeting the m. biceps brachii once a week or more over the past year

Sponsors & Collaborators

• Norwegian School of Sport Sciences: lead
• Université de Nantes: collaborator
• Oslo University Hospital: collaborator
• University of Oslo: collaborator
• Syddansk Universitet, Denmark: collaborator
• University of Copenhagen: collaborator

Study Sites (1)

Norwegian School of Sport Sciences

Oslo, 0863, Norway

Location

Study Officials

• Truls Raastad, PhD

  Norwegian School of Sport Sciences

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: Participants perform one bout of eccentric exercise with one arm, while the contralateral arm serves as control. Which arm who receives eccentric exercise is randomized between dominant/non-dominant arm.

Study Record Dates

• First Submitted: May 10, 2021
• First Posted: September 5, 2021
• Study Start: December 3, 2019
• Primary Completion: October 20, 2020
• Study Completion: December 20, 2020
• Last Updated: November 30, 2023

Record last verified: 2023-11

Data Sharing

• IPD Sharing: Will not share

Locations

NO (1)