Effect of Branched-chain Amino Acid Supplementation on Muscle Damage

NCT03766815 | Unknown DMC

• 1 other identifier: 113281
• Study Type: interventional
• Target: 78
• Locations: 1 country
• Sites: 1

Brief Summary

Seventy-eight recreational athletes who never resistance trained will be randomly assigned into three groups of twenty-six. Branched-chain amino acid will be supplemented for 18days at 0, 200 or 400 mg/body weight(kg)/day in jelly. Participants will be asked to perform elbow flexions on the 12th day of supplementation. Maximum voluntary contractions will be measured before, during and after the supplementation period to compare the effects of different doses of branched-chain amino acid has on muscle damage markers.

Conditions

Muscle Damage

Outcome Measures

Primary Outcomes (1)

• Change in maximum voluntary contraction (MVC)

  Participants will be asked to perform three repetitions of concentric elbow flexion at their maximal force (1RM) with both their dominant and non-dominant arm on an isokinetic dynamometer (Biodex).

  baseline (before supplementation) and at 1,3,24,48,72,96,120,144 hours after muscle damage

Secondary Outcomes (9)

• Change in blood myoglobin

  10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage

• Change in 3-methyl-histidine

  10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage

• Changes in insulin

  10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage

• Change in muscle soreness

  10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage

• Change in ultrasound images of the arm muscles

  10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage

Study Arms (3)

Placebo

PLACEBO COMPARATOR

Fiber supplement mixed with jelly will be ingested for 18 days with participants performing elbow eccentric contractions as a muscle damaging exercise on the 12th-day of supplementation.

Dietary Supplement: Placebo; Behavioral: Muscle damaging exercise

BCAA 200mg/kg/day

EXPERIMENTAL

Branched-chain amino acid supplement mixed with jelly will be ingested for 18 days. The amount of supplement provided will be based on body weight (200mg/kg/day) with participants performing elbow eccentric contractions as a muscle damaging exercise on the 12th-day of supplementation.

Dietary Supplement: BCAA 200mg/kg/day; Behavioral: Muscle damaging exercise

BCAA 400mg/kg/day

EXPERIMENTAL

Branched-chain amino acid supplement mixed with jelly will be ingested for 18 days. The amount of supplement provided will be based on body weight (400mg/kg/day) with participants performing elbow eccentric contractions as a muscle damaging exercise on the 12th-day of supplementation.

Dietary Supplement: BCAA 400mg/kg/day; Behavioral: Muscle damaging exercise

Interventions

BCAA 200mg/kg/day DIETARY_SUPPLEMENT

BCAA supplement based on 200mg/kg/day for 18 days.

BCAA 200mg/kg/day

Placebo DIETARY_SUPPLEMENT

Fiber supplement jelly for 18 days.

Placebo

BCAA 400mg/kg/day DIETARY_SUPPLEMENT

BCAA supplement based on 400mg/kg/day for 18 days.

BCAA 400mg/kg/day

Muscle damaging exercise BEHAVIORAL

Participants will be asked to perform muscle damaging elbow eccentric contraction exercise at 120% of 1RM for three sets of 15 repetitions with the dominant arm at maximal effort on a Biodex machine with a 3 minute rest period between each set.

BCAA 200mg/kg/day; BCAA 400mg/kg/day; Placebo

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy males and females at the age of 18-40
• Endurance trained athletes
• Weekly exercise duration is less than 150minutes but more than 30 minutes

You may not qualify if:

• Individuals who cannot endure three days for not eating meat
• Diabetic
• Have been performing resistance trainings
• Have been taking protein supplement
• Have been consuming a high level of protein in the regular diet
• Have been taking omega-three and vitamin E supplements
• Have been taking anabolic Steroids
• Have been taking regular medications
• Have cardiovascular Disease
• Have a history of joint \& Muscle Injuries
• Females with irregular Menstrual Cycle

Sponsors & Collaborators

• Western University, Canada: lead

Study Sites (1)

Exercise Nutrition Laboratory (Western University)

London, Ontario, N6A 3K7, Canada

RECRUITING

Study Officials

• Peter Lemon, PhD

  Western University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Crystal Lee, BSc

CONTACT

519 6612111; plee263@uwo.ca

Arash Bandegan, PhD

CONTACT

519 6612111; abandeg@uwo.ca

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Masking Details: Participants will not be able to distinguish branched-chain amino acid and fiber supplement by taste, color or scent as the supplements are provided in jelly.
• Purpose: OTHER
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Model Details: Participants will be supplemented for 18 days with either 200mg BCAA/kg/day, 400mg BCAA/kg/day or placebo (fiber supplement) in jelly in a single-blind fashion. Followed by a muscle damaging exercise and subsequent MVC and blood sample for force and metabolite measurements.

Study Record Dates

• First Submitted: December 3, 2018
• First Posted: December 6, 2018
• Study Start: October 1, 2019
• Primary Completion: December 31, 2019
• Study Completion: December 31, 2019
• Last Updated: October 2, 2019

Record last verified: 2019-09

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)