Evaluation of Biomarker Levels in Blood, Saliva and Gingival Fluid in Periodontitis

NCT04647123 | Unknown

• 1 other identifier: : 06.06.2018/09
• Study Type: observational
• Target: 40
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study was to compare IMA, MDA, CAT, GSH and ADA levels in gingival crevicular fluid, serum and saliva of periodontally hopeless, periodontitis and gingivitis teeth with healthy control groups.

Conditions

Periodontitis

Keywords

periodontitis; catalase; malondialdehide

Outcome Measures

Primary Outcomes (5)

• adenosine deaminase

  Adenosine deaminase (ADA) is an enzyme involved in proliferation and differentiation. Although ADA has been investigated in different inflammatory and infectious diseases and has been defined as an immunoenzyme biomarker, its role in periodontitis has not been fully elucidated yet.

  1 year

• catalase

  Catalase (CAT) decomposes H2O2 catalyzed by superoxide dismutase (SOD) into H2O and O2. (13) It is stated in the literature that CAT, SOD and total oxidant state are important biomarkers that can be used in the diagnosis of periodontal disease and evaluation of treatment results. (14)

  1 year

• Ischemia-modified albumin

  Ischemia-modified albumin (IMA) is suggested as an oxidative stress marker and an indicator of inflammatory diseases. It has been reported in the literature that the serum IMA level increases significantly in the case of periodontal disease and decreases after treatment. In this respect, it has been stated that IMA can be an indicator of systemic inflammation caused by periodontal disease.

  1 year

• malondialdehide

  In periodontal disease, an increase in reactive oxygen species and a decrease in antioxidant activity are observed because this disease is thought to be associated with an imbalance between antioxidants and oxidants. (8) Lipid peroxidation is a result of periodontitis. Free radicals that lead to the production of toxic and reactive aldehyde metabolites such as malondialdehyde (MDA) cause lipid peroxidation. (9) It is among the information in the literature that MDA levels increase in the case of periodontal inflammation and that MDA can be considered as an oxidative stress marker for periodontitis. (10-12)

  1 year

• glutathione

  Glutathione (GSH) acts as a coenzyme in the oxidation-reduction process. (15) GSH is a critical redox regulator and important for the health of peridontium

  1 year

Study Arms (4)

Hopeless teeth

The teeth that can not be treated periodontally and who are desperate for extraction will be included in this group.

Other: No intervention was applied to the groups the samples will be collected in terms of the diagnosis (hopeless, periodontitis, gingivitis, healthy)

Periodontitis

Teeth diagnosed with periodontitis will be included in this group.

Other: No intervention was applied to the groups the samples will be collected in terms of the diagnosis (hopeless, periodontitis, gingivitis, healthy)

Gingivitis

Teeth diagnosed with gingivitis will be included in this group.

Other: No intervention was applied to the groups the samples will be collected in terms of the diagnosis (hopeless, periodontitis, gingivitis, healthy)

Healthy

Teeth diagnosed with helthy will be included in this group.

Other: No intervention was applied to the groups the samples will be collected in terms of the diagnosis (hopeless, periodontitis, gingivitis, healthy)

Interventions

No intervention was applied to the groups the samples will be collected in terms of the diagnosis (hopeless, periodontitis, gingivitis, healthy) OTHER

No intervention was applied to the groups the samples will be collected in terms of the diagnosis (hopeless, periodontitis, gingivitis, healthy)

Gingivitis; Healthy; Hopeless teeth; Periodontitis

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Systemically healhty ,non-smokers,non-pregnants will be included in the study

You may qualify if:

• Systemically healhty non-smokers non-pregnants -

You may not qualify if:

• \- Patients with systemic disease smokers pregnants

Sponsors & Collaborators

• Yuzuncu Yil University: lead

Study Sites (1)

Van yuzuncu yil university

Van, 65080, Turkey (Türkiye)

RECRUITING

MeSH Terms

Conditions

Periodontitis

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Officials

• hacer sahin aydınyurtt, assoc. prof

  Yuzuncu Yil University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Hacer sahin aydınyurt

CONTACT

05304589710; hacersahinay@gmail.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Target Duration: 15 Months
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assoc. Prof.

Study Record Dates

• First Submitted: November 17, 2020
• First Posted: November 30, 2020
• Study Start: January 1, 2020
• Primary Completion: February 1, 2021
• Study Completion: March 1, 2021
• Last Updated: November 30, 2020

Record last verified: 2020-11

Locations

TU (1)