Netrin 1 and Its Receptor Unc5b as Markers of Periodontal Disease
Levels of Netrin 1 and Its Receptor Unc5b in Gingival Crevicular Fluid, Saliva and Serum in Periodontal Disease and Health
2 other identifiers
interventional
60
1 country
1
Brief Summary
This study aimed to investigate gingival crevicular fluid (GCF), saliva and serum Netrin 1 and Unc5b levels in periodontal health and disease. A total of 60 individuals, 20 patients with periodontitis, 20 with gingivitis and 20 periodontally healthy individuals were included. Whole-mouth and site-specific clinical periodontal parameters including probing depth, clinical attachment level, bleeding on probing, gingival index, plaque index and papillar bleeding index were recorded. GCF, salivary and serum Netrin 1 and Unc5b levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was performed by using non-parametric tests.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P25-P50 for not_applicable
Started Jan 2018
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
January 1, 2018
CompletedPrimary Completion
Last participant's last visit for primary outcome
February 1, 2019
CompletedStudy Completion
Last participant's last visit for all outcomes
April 1, 2019
CompletedFirst Submitted
Initial submission to the registry
April 15, 2019
CompletedFirst Posted
Study publicly available on registry
April 18, 2019
CompletedApril 18, 2019
April 1, 2019
1.1 years
April 15, 2019
April 17, 2019
Conditions
Keywords
Outcome Measures
Primary Outcomes (1)
Biochemical parameters (Netrin-1 and Unc5b)
The changes in levels of Netrin-1 and Unc5b 4 weeks after periodontal treatment determined by ELISA. The changes in levels of Netrin-1 and Unc5b were analyzed to determine as a diagnostic biomarker of periodontal disease.
Baseline and 4 weeks after treatment
Secondary Outcomes (6)
Probing pocket depth
Baseline and 4 weeks after treatment
Clinical attachment loss
Baseline and 4 weeks after treatment
Gingival index
Baseline and 4 weeks after treatment
Plaque index
Baseline and 4 weeks after treatment
Bleeding on probing
Baseline and 4 weeks after treatment
- +1 more secondary outcomes
Study Arms (3)
Periodontitis
ACTIVE COMPARATORGCF, saliva and serum samples were taken before and after treatment from periodontitis patients. Intervention: Non- surgical periodontal treatment (SRP and oral hygiene instructions)
Gingivitis
ACTIVE COMPARATORGCF, saliva and serum samples were taken before and after treatment from gingivitis patients. Intervention: Non- surgical periodontal treatment (Scaling and oral hygiene instructions)
Periodontally healthy
PLACEBO COMPARATORGCF, saliva and serum samples were taken at baseline from periodontally healthy individuals.
Interventions
SRP under local anaesthesia, in a total of 2 clinical visits. Oral hygiene instructions including the modified Bass technique and an appropriate interdental cleaning device with dental floss and interdental brush.
GCF with filter paper using the intracrevicular method, saliva and serum collection
Eligibility Criteria
You may qualify if:
- Aged \>18 years,
- Having at least 16 natural teeth (excluding third molar),
- Nonsmokers with no history of smoking,
- Not having any diagnosed medical illness or drug intake that could affect the periodontal condition.
You may not qualify if:
- Patients who had taken antibiotics, nonsteroidal antiinflammatory or any other drugs within the past 3 months,
- Patients received nonsurgical or surgical periodontal treatment,
- Patients who have a restorative and endodontic therapy requirement,
- Having a removable partial denture and/or having orthodontic therapy,
- Current pregnancy or lactation and having serum CRP \> 3mg/L .
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
Bolu Abant Izzet Baysal University
Bolu, 14030, Turkey (Türkiye)
Related Publications (2)
Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, Flemmig TF, Garcia R, Giannobile WV, Graziani F, Greenwell H, Herrera D, Kao RT, Kebschull M, Kinane DF, Kirkwood KL, Kocher T, Kornman KS, Kumar PS, Loos BG, Machtei E, Meng H, Mombelli A, Needleman I, Offenbacher S, Seymour GJ, Teles R, Tonetti MS. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol. 2018 Jun;89 Suppl 1:S173-S182. doi: 10.1002/JPER.17-0721.
PMID: 29926951BACKGROUNDCaton JG, Armitage G, Berglundh T, Chapple ILC, Jepsen S, Kornman KS, Mealey BL, Papapanou PN, Sanz M, Tonetti MS. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. J Clin Periodontol. 2018 Jun;45 Suppl 20:S1-S8. doi: 10.1111/jcpe.12935.
PMID: 29926489BACKGROUND
MeSH Terms
Conditions
Condition Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Sadiye Gunpinar, Asst. Prof.
Abant Izzet Baysal University
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- NON RANDOMIZED
- Masking
- SINGLE
- Who Masked
- OUTCOMES ASSESSOR
- Masking Details
- Single (Outcomes Assessor)
- Purpose
- DIAGNOSTIC
- Intervention Model
- PARALLEL
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Asst. Prof.
Study Record Dates
First Submitted
April 15, 2019
First Posted
April 18, 2019
Study Start
January 1, 2018
Primary Completion
February 1, 2019
Study Completion
April 1, 2019
Last Updated
April 18, 2019
Record last verified: 2019-04