GCF Levels of IL-1β and IL-6 in Gingivitis and Stage III-Grade C Periodontitis

NCT04689438 | Completed

• 1 other identifier: UsakU7
• Study Type: observational
• Target: 64
• Locations: 1 country
• Sites: 1

Brief Summary

Periodontal diseases are chronic diseases that occur as a result of a violation of the balance between microbial dental plaque and the host response. Gingivitis is a disease characterized by inflammation of the gingiva that occurs in one or more areas without loss of attachments.1 in periodontitis, an inflammatory event that begins in the gingiva along with gingivitis spreads to the periodontal ligament, alveolar bone and soft tissues that support the tooth, causing the destruction of these structures.2 Cytokines are low molecular weight proteins that participate in the initial and active stages of inflammation and immunity. In periodontal disease pathogenesis, cytokine response has been reported to play a very critical role in determining disease progression.3 IL-1beta and IL-6 are key cytokines in chronic inflammatory diseases and have the potential to initiate bone loss and tissue destruction seen in periodontal disease.4the purpose of this study; it is to determine the degree of inflammation and periodontal destruction by determining the levels of IL-1beta and IL-6 cytokines in the gingival crevicular fluid of periodontal healthy and diseased individuals.

Conditions

Periodontitis

Keywords

periodontal disease; periodontitis; gingivitis; cytokines

Outcome Measures

Primary Outcomes (2)

• Measurement of IL-1Beta level

  GCF was taken interproximal region.

  GCF was taken within 1 week after clinical examination

• Measurement of IL-6 level in GCF

  GCF was taken interproximal region.

  GCF was taken within 1 week after clinical examination

Study Arms (3)

Healthy control groups

clinically healthy gingiva BOP score less than 10%

Diagnostic Test: gingival crevicular fluid

Gingivitis groups

BOP score of 10% or greater

Diagnostic Test: gingival crevicular fluid

Periodontitis groups

interdental AL ≥5 mm, PD≥6 mm

Diagnostic Test: gingival crevicular fluid

Interventions

gingival crevicular fluid DIAGNOSTIC_TEST

GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.

Gingivitis groups; Healthy control groups; Periodontitis groups

Eligibility Criteria

• Age: 20 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

individuals who have periodontal disease will be selected

You may qualify if:

• have periodontitis
• have 16 permanent teeth

You may not qualify if:

• Systemic disease
• using drugs

Sponsors & Collaborators

• Uşak University: lead

Study Sites (1)

University of Usak

Uşak, 64200, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Gingival crevicular fluid

MeSH Terms

Conditions

Periodontitis; Periodontal Diseases; Gingivitis

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases; Infections; Gingival Diseases

Study Officials

• ahu dikilitaş

  University of Usak

  PRINCIPAL INVESTIGATOR

• fatih karaaslan

  University of Usak

  STUDY CHAIR

• abdullah seçkin ertuğrul

  University of IZMIR KATİP CELEBİ

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Usak University Faculty of Dentistry

Study Record Dates

• First Submitted: December 28, 2020
• First Posted: December 30, 2020
• Study Start: October 8, 2020
• Primary Completion: December 25, 2020
• Study Completion: December 28, 2020
• Last Updated: November 4, 2022

Record last verified: 2022-11

Locations

TU (1)