Microbiome and Proteome Mapping in Periodontitis

NCT06675474 | Completed

• 1 other identifier: All-Site Mapping
• Study Type: observational
• Target: 3
• Locations: 1 country
• Sites: 1

Brief Summary

This study aimed to compare the microbiome and proteome profiles of saliva, serum and all periodontal sites in patients with periodontitis. Saliva, serum, gingival crevicular fluid and subgingival plaque were obtained from three patients with stage III, grade C periodontitis. Quantitative proteomics were performed for proteome analysis of saliva, serum and gingival crevicular fluid, whereas shotgun whole genome sequencing was performed for microbiome analysis in saliva, serum and plaque.

Conditions

Periodontitis

Outcome Measures

Primary Outcomes (2)

• Host proteomics profiles from saliva, serum and GCF

  Concentrations

  24 hours after clinical periodontal measurements

• Microbial profiles from subgingival plaque and saliva

  Linear discriminant analysis scores

  24 hours after clinical periodontal measurements

Study Arms (1)

Periodontitis

These patients had at least two interdental sites (at two non-adjacent teeth) with interproximal CAL ≥5 mm, along with PD ≥6 mm and RBL extending to the mid-third of the root or beyond. CAL was not originated from endodontic lesions draining through the marginal periodontium, dental caries extending into the cervical areas of the teeth, trauma-related gingival recession or distal bone loss in 2nd molars owing to extractions of 3rd molars. They had ≤4 teeth lost due to periodontitis.

Other: Clinical periodontal measurements; Other: Saliva sampling; Other: Serum Sampling; Other: GCF sampling; Other: Subgingival plaque sampling

Interventions

Clinical periodontal measurements OTHER

Clinical periodontal measurements included PD, CAL, BOP (+/-), GI, and PI. Clinical recordings were performed at six points (mesiobuccal, buccal, distobuccal, mesiopalatal, palatal, and distopalatal) of all teeth, except the 3rd molars.

Periodontitis

Saliva sampling OTHER

The whole unstimulated saliva samples were collected. Each participant was asked first to rinse the mouth completely with tap water for 2 minutes, wait for 10 minutes. Then the participants were requested to let the saliva pool in their floor of the mouth and to allow the saliva to drain passively into a falcon tube, 5 minutes.

Periodontitis

Serum Sampling OTHER

5 ml of venous blood were taken from the antecubital vein by a standard venipuncture method.

Periodontitis

GCF sampling OTHER

GCF was collected from the six sites (mesiobuccal, buccal, distobuccal, mesiopalatal, palatal, and distopalatal) per tooth, except the 3rd molars, via steril paper strips.

Periodontitis

Subgingival plaque sampling OTHER

Subgingival plaque was collected by inserting two standardized paper points (no:30) at six sites (mesiobuccal, buccal, distobuccal, mesiopalatal, palatal, and distopalatal) per tooth, except the 3rd molars.

Periodontitis

Eligibility Criteria

• Age: 43 Years - 60 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

A total of three systemically healthy and non-smoking patients with stage III, grade C peridontitis (3 males; age range 43 to 60 years) were consecutively enrolled from the population seeking periodontal treatment for the first time at the Department of Periodontology, School of Dentistry, Aydın Adnan Menderes University, Aydın, Turkey, between December 2019 to February 2020.

You may qualify if:

• No history of smoking
• At least 24 natural teeth (excluding 3rd molars)

You may not qualify if:

• Being diagnosed with diabetes mellitus, rheumatoid arthritis, cardiovascular system diseases, endocrine, immunologic, and mucocutaneous disorders
• Use of antibiotics, antihypertensives immunosuppressive, anti-inflammatory drugs and topical antiseptic solutions within the past 6 months
• Having periodontal treatment in the previous year
• Wearing removable partial dentures or orthodontic appliances
• Pregnant or nursing women

Sponsors & Collaborators

• Aydin Adnan Menderes University: lead

Study Sites (1)

Aydın Adnan Menderes University, Faculty of Dentistry, Department of Periodontology

Aydin, 09100, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Saliva, serum, gingival crevicular fluid and subgingival plaque

MeSH Terms

Conditions

Periodontitis

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Officials

• Nagihan Bostanci

  Karolinska Institutet

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assoc. Prof.

Study Record Dates

• First Submitted: November 4, 2024
• First Posted: November 5, 2024
• Study Start: December 4, 2019
• Primary Completion: February 12, 2020
• Study Completion: May 15, 2020
• Last Updated: November 5, 2024

Record last verified: 2024-11

Locations

TU (1)