Food First Approach to Stimulate Muscle Protein Synthesis in Healthy Adults

NCT03870165 | Completed

• 1 other identifier: 19293
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

In a crossover design 10 young healthy adults (20-35 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after exercise participants will ingest either 3.5 oz of Salmon fillet or its constituent macronutrients as isolated amino acids and fat. Repeated blood and breath samples as well as muscle biopsies will be collected to determine whole body amino acid kinetics, muscle amino acid transporters, anabolic signalling and myofibrillar protein synthesis rates during the trials

Conditions

Hypertrophy

Outcome Measures

Primary Outcomes (1)

• Fractional synthetic rate of myofibrillar proteins

  Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 5 hr after the ingestion of the experimental interventions. This will allow us to assess the change from the postabsorptive to the postprandial period.

  Postabsorptive for 3 hours, postprandial for 5 hours.

Secondary Outcomes (2)

• Phosphorylation of muscle anabolic signaling

  Baseline and at 2 and 5 hours after protein ingestion

• Exogenous rate of leucine appearance

  5 hour postprandial phase

Study Arms (2)

Salmon

ACTIVE COMPARATOR

After resistance exercise, participants will ingest 3.5 oz of salmon fillet (21g protein, 24g fat) cooked sous-vide.

Behavioral: Resistance exercise; Other: Salmon

Isolated mixture

EXPERIMENTAL

After resistance exercise, participants will ingest an isolated amino acid and fatty acid mixture matched to the amino acid and fatty acid content of 3.5 oz salmon fillet.

Behavioral: Resistance exercise; Dietary Supplement: Isolated amino acids and fatty acids

Interventions

Resistance exercise BEHAVIORAL

Participants will perform leg press and leg extension immediately prior to ingestion of salmon or isolated mixture

Isolated mixture; Salmon

Salmon OTHER

Participants will ingest salmon immediately after resistance exercise

Salmon

Isolated amino acids and fatty acids DIETARY_SUPPLEMENT

Participants will ingest a mixture of isolated amino acids and fatty acids immediately after resistance exercise

Isolated mixture

Eligibility Criteria

• Age: 20 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age 20-35 years
• Recreationally-active adults: ≥ 30 min of physical activity at moderate intensity ≥ 3 times per week
• English fluency

You may not qualify if:

• Smoking
• Known allergies to fish consumption
• Vegans
• Diagnosed GI tract diseases
• Arthritic conditions
• A history of neuromuscular problems
• Diagnosed cognitive impairments
• Recent (1 year) participation in amino acid tracer studies
• Predisposition to hypertrophic scarring or keloid formation
• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
• High blood pressure (Systolic \> 140 mm HG; Diastolic \> 90 mm HG)
• Alcohol consumption \>10 drinks per week
• Metabolic disorders (e.g., Metabolic Syndrome, diabetes, thyroid diseases)
• Consumption of thyroid, androgenic or other medications known to affect endocrine function
• Consumption of ergogenic-leves of dietary supplements that may affect muscle mass (e.g., creatine, HMB), insulin-like substances, or anabolic/catabolic pro-hormones (e.g., DHEA) within 6 weeks prior to participation

Sponsors & Collaborators

• University of Illinois at Urbana-Champaign: lead

Study Sites (1)

Freer Hall

Urbana, Illinois, 61801, United States

Location

Related Publications (1)

• Paulussen KJ, Barnes TM, Askow AT, Salvador AF, McKenna CF, Scaroni SE, Fliflet A, Ulanov AV, Li Z, West DW, Bauer LL, Paluska SA, Dilger RN, Moore DR, Boppart MD, Burd NA. Underpinning the Food Matrix Regulation of Postexercise Myofibrillar Protein Synthesis by Comparing Salmon Ingestion With the Sum of Its Isolated Nutrients in Healthy Young Adults. J Nutr. 2023 May;153(5):1359-1372. doi: 10.1016/j.tjnut.2023.02.037. Epub 2023 Mar 2.

  PMID: 36870539 DERIVED

MeSH Terms

Conditions

Hypertrophy

Interventions

Resistance Training; Fatty Acids

Condition Hierarchy (Ancestors)

Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Lipids

Study Officials

• Nicholas A Burd, PhD

  University of Illinois at Urbana-Champaign

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: March 8, 2019
• First Posted: March 11, 2019
• Study Start: March 1, 2019
• Primary Completion: November 22, 2019
• Study Completion: November 22, 2019
• Last Updated: September 28, 2020

Record last verified: 2020-09

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)