NCT03117127

Brief Summary

In crossover trials, ten (N=10) young men (18-35 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after resistance exercise, participants will ingest stable isotope labeled whole eggs (18 g protein, 17 g fat) or egg whites (18 g protein, 0 g fat) cooked in scrambled form. Repeated blood and muscle biopsies will be collected to determine whole body amino acid kinetics, muscle amino acid transporters, anabolic signaling and myofibrillar protein synthesis rates during the trials.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
10

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Jul 2015

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

July 1, 2015

Completed
1.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 1, 2017

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

April 10, 2017

Completed
7 days until next milestone

First Posted

Study publicly available on registry

April 17, 2017

Completed
14 days until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2017

Completed
Last Updated

May 1, 2018

Status Verified

April 1, 2018

Enrollment Period

1.6 years

First QC Date

April 10, 2017

Last Update Submit

April 30, 2018

Conditions

Hypertrophy

Outcome Measures

Primary Outcomes (1)

  • Fractional synthetic rate of myofibrillar proteins to whole egg and egg white ingestion

    Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 5 hr after the ingestion of the experimental beverages. This will allow us to assess the change from the postabsorptive to the postprandial period

    Postabsorptive for 3 hours, Postprandial for 5 hours

Secondary Outcomes (2)

  • Phosphorylation of muscle anabolic signaling

    Baseline and at 2 and 5 hours after protein ingestion

  • Exogenous rate of leucine appearance after ingestion of whole eggs and egg whites.

    5 hour postprandial phase

Study Arms (2)

Whole eggs

ACTIVE COMPARATOR

After resistance exercise, participants will ingest whole eggs (18 g protein, 17 g fat) cooked in scrambled form.

Behavioral: Resistance ExerciseOther: Whole Eggs

Egg Whites

EXPERIMENTAL

After resistance exercise, participants will ingest egg whites (18 g protein, 0 g fat) cooked in scrambled form.

Behavioral: Resistance ExerciseOther: Egg Whites

Interventions

Resistance ExerciseBEHAVIORAL

Participants will perform leg extension and leg press immediately prior to ingestion of whole eggs or egg whites

Egg WhitesWhole eggs
Whole EggsOTHER

Participants will ingestion whole eggs immediately after resistance exercise

Whole eggs
Egg WhitesOTHER

Participants will ingestion whole eggs immediately after resistance exercise

Egg Whites

Eligibility Criteria

Age18 Years - 35 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Male
  • Aged 18-35 years
  • Healthy, active (self-reported to exercise 2 - 4 times per week)
  • BMI \> 18.5 and \< 25 kg/m2

You may not qualify if:

  • Smoking
  • Known allergies to egg consumption
  • Vegans
  • Diagnosed GI tract diseases
  • Arthritic conditions
  • A history of neuromuscular problems
  • Diagnosed cognitive impairments
  • Recent (1 year) participation in amino acid tracer studies
  • Predisposition to hypertrophic scarring or keloid formation
  • Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
  • High blood pressure (Systolic \> 140 mm HG; Diastolic \> 90 mm HG)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Freer Hall

Urbana, Illinois, 61801, United States

Location

Related Publications (1)

  • van Vliet S, Shy EL, Abou Sawan S, Beals JW, West DW, Skinner SK, Ulanov AV, Li Z, Paluska SA, Parsons CM, Moore DR, Burd NA. Consumption of whole eggs promotes greater stimulation of postexercise muscle protein synthesis than consumption of isonitrogenous amounts of egg whites in young men. Am J Clin Nutr. 2017 Dec;106(6):1401-1412. doi: 10.3945/ajcn.117.159855. Epub 2017 Oct 4.

    PMID: 28978542DERIVED

MeSH Terms

Conditions

Hypertrophy

Interventions

Resistance TrainingEgg White

Condition Hierarchy (Ancestors)

Pathological Conditions, AnatomicalPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Exercise TherapyRehabilitationAftercareContinuity of Patient CarePatient CareTherapeuticsPhysical Therapy ModalitiesPhysical Conditioning, HumanExerciseMotor ActivityMovementMusculoskeletal Physiological PhenomenaMusculoskeletal and Neural Physiological PhenomenaEggsFoodDiet, Food, and NutritionPhysiological PhenomenaFood and Beverages

Study Officials

  • Nicholas A Burd, Ph.D.

    Assistant Professor

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
PARTICIPANT
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant Professor

Study Record Dates

First Submitted

April 10, 2017

First Posted

April 17, 2017

Study Start

July 1, 2015

Primary Completion

February 1, 2017

Study Completion

May 1, 2017

Last Updated

May 1, 2018

Record last verified: 2018-04

Data Sharing

IPD Sharing
Will not share

Locations

US(1)