Influence of Exercise on Trans Fatty Acids

NCT03170973 | Unknown DMC

• 1 other identifier: 12345
• Study Type: interventional
• Target: 66
• Locations: 1 country
• Sites: 1

Brief Summary

Introduction: The metabolism of fatty acids in plasma is modulated by their availability in plasma. Individuals with increased weight have increased plasma fatty acids and physical exercise seems to favor the metabolic responses of fatty acid mobilization. Objective: To test the hypothesis that the physical exercise of acute way changes the fatty acids of medium chain of the serum of individuals with increase of the corporal weight. Method: Including 66 women, randomly divided into two groups, control and experiment, overweight, sedentary, and between 18 and 30 years of age. After a 12-hour fast, basal blood collection will be performed. The experiment group, 12 hours after the first collection, will be submitted to a physical exercise session with energy expenditure of 250Kcal. The volunteers in the control and experiment group will make a second blood collection 24 hours after the first one. The fatty acids will be dosed: pelargonic, azelaic, elaidic and oleic by gas chromatography. Intra and intergroup comparisons will be made using the t test for independent and dependent samples, p \<0.05.

Conditions

Obesity; Motor Activity; Fatty Acids; Lipid

Keywords

Obesity; Motor Activity; Fatty acids; Lipid

Outcome Measures

Primary Outcomes (1)

• Levels of fatty acids in individuals with changes in body weight before in the control and experimental group

  After blood collection, the initial step for analysis of fatty acids will be the transesterification of the samples through two steps: extraction and hydrolysis / esterification. Similarly, the 99% purity standards of the fatty acids (Pelargonium, azelaic, Oleic and elaidic will also be transesterified. After the transesterification of the standards and samples, they will be analyzed by gas chromatography.

  30 days

Secondary Outcomes (1)

• Plasma levels of clinical variables (lipid profile, glycemic profile, oxidative and inflammatory stress) before and after physical activity.

  3 months

Study Arms (2)

Comparison of fatty acids before and after exercise

EXPERIMENTAL

Other: Exercise

Comparison of fatty acids at baseline and 24 hours after

NO INTERVENTION

Interventions

Exercise OTHER

After a 12-hour fast, volunteers will collect blood in the antecubital vein to measure basal serum values. 12 after the first blood collection they will perform a physical exercise session on a treadmill. The same will be divided in 3 times: heating, conditioning and cooling. The heating will be of 7 minutes, the cooling of 5 minutes and the time of conditioning will correspond to the energy expenditure of 250Kcal with light intensity based on Borg's perception of effort, that is, in the original scale a value between 9 and 11. After 24 hours after the first blood collection the volunteers will return to the laboratory after a 12-hour fast and blood samples will be collected again.

Comparison of fatty acids before and after exercise

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• women
• Body mass index (BMI) over 24.9kg / m2
• years
• Sedentary

You may not qualify if:

• cardiovascular disease,
• metabolic disease (diabetes, dyslipidemias)
• history of alcoholism or smoking,
• use of lipid-lowering agents,
• use of corticosteroids,
• uses of diuretics,
• use of beta-blockers,
• use of contraceptives,
• use of hypothyroidism,
• use of parenchymal renal diseases

Sponsors & Collaborators

• Faculdade Adventista da Bahia: lead
• Conselho Nacional de Desenvolvimento Científico e Tecnológico: collaborator

Study Sites (1)

Djeyne Silveira Wagmacker

Cachoeira, Estado de Bahia, 44300---, Brazil

RECRUITING

Related Publications (14)

• Holloway GP, Lally J, Nickerson JG, Alkhateeb H, Snook LA, Heigenhauser GJ, Calles-Escandon J, Glatz JF, Luiken JJ, Spriet LL, Bonen A. Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle. J Physiol. 2007 Jul 1;582(Pt 1):393-405. doi: 10.1113/jphysiol.2007.135301. Epub 2007 May 3.

  PMID: 17478525 BACKGROUND

• Jayewardene AF, Mavros Y, Reeves A, Hancock DP, Gwinn T, Rooney KB. Interactions Between Fatty Acid Transport Proteins, Genes That Encode for Them, and Exercise: A Systematic Review. J Cell Physiol. 2016 Aug;231(8):1671-87. doi: 10.1002/jcp.25281. Epub 2016 Feb 2.

  PMID: 26638980 RESULT

• Bradley NS, Snook LA, Jain SS, Heigenhauser GJ, Bonen A, Spriet LL. Acute endurance exercise increases plasma membrane fatty acid transport proteins in rat and human skeletal muscle. Am J Physiol Endocrinol Metab. 2012 Jan 15;302(2):E183-9. doi: 10.1152/ajpendo.00254.2011. Epub 2011 Oct 25.

  PMID: 22028411 RESULT

• Barres R, Yan J, Egan B, Treebak JT, Rasmussen M, Fritz T, Caidahl K, Krook A, O'Gorman DJ, Zierath JR. Acute exercise remodels promoter methylation in human skeletal muscle. Cell Metab. 2012 Mar 7;15(3):405-11. doi: 10.1016/j.cmet.2012.01.001.

  PMID: 22405075 RESULT

• Jabbour G, Iancu HD, Paulin A, Lavoie JM, Lemoine-Morel S, Zouhal H. Effects of Acute Supramaximal Cycle Exercise on Plasma FFA Concentration in Obese Adolescent Boys. PLoS One. 2015 Jun 15;10(6):e0129654. doi: 10.1371/journal.pone.0129654. eCollection 2015.

  PMID: 26076464 RESULT

• Jabbour G, Iancu HD, Paulin A. Effects of High-Intensity Training on Anaerobic and Aerobic Contributions to Total Energy Release During Repeated Supramaximal Exercise in Obese Adults. Sports Med Open. 2015;1(1):36. doi: 10.1186/s40798-015-0035-7. Epub 2015 Oct 20.

  PMID: 26512339 RESULT

• Plaisance EP, Mestek ML, Mahurin AJ, Taylor JK, Moncada-Jimenez J, Grandjean PW. Postprandial triglyceride responses to aerobic exercise and extended-release niacin. Am J Clin Nutr. 2008 Jul;88(1):30-7. doi: 10.1093/ajcn/88.1.30.

  PMID: 18614721 RESULT

• Chen MJ, Fan X, Moe ST. Criterion-related validity of the Borg ratings of perceived exertion scale in healthy individuals: a meta-analysis. J Sports Sci. 2002 Nov;20(11):873-99. doi: 10.1080/026404102320761787.

  PMID: 12430990 RESULT

• Santos RD, Gagliardi AC, Xavier HT, Magnoni CD, Cassani R, Lottenberg AM; Sociedade Brasileira de Cardiologia; Arpadi Faludi A, Geloneze B, Scherr C, Kovacs C, Tomazzela C, Carla C, Barrera-Arellano D, Cintra D, Quintao E, Nakandakare ER, Fonseca FA, Pimentel I, Ernesto dos Santos J, Bertolami MC, Rogero M, Izar MC, Nakasato M, Teixeira Damasceno NR, Maranhao R, Cassani RS, Perim R, Ramos S; Sociedade Brasileira de Cardiologia. [First guidelines on fat consumption and cardiovascular health]. Arq Bras Cardiol. 2013 Jan;100(1 Suppl 3):1-40. No abstract available. Portuguese.

  PMID: 23598539 RESULT

• Garelnabi M, Litvinov D, Parthasarathy S. Evaluation of a gas chromatography method for azelaic acid determination in selected biological samples. N Am J Med Sci. 2010 Sep;2(9):397-402. doi: 10.4297/najms.2010.2397.

  PMID: 22558586 RESULT

• Miyamoto S, Taylor SL, Barupal DK, Taguchi A, Wohlgemuth G, Wikoff WR, Yoneda KY, Gandara DR, Hanash SM, Kim K, Fiehn O. Systemic Metabolomic Changes in Blood Samples of Lung Cancer Patients Identified by Gas Chromatography Time-of-Flight Mass Spectrometry. Metabolites. 2015 Apr 9;5(2):192-210. doi: 10.3390/metabo5020192.

  PMID: 25859693 RESULT

• Magkos F, Wright DC, Patterson BW, Mohammed BS, Mittendorfer B. Lipid metabolism response to a single, prolonged bout of endurance exercise in healthy young men. Am J Physiol Endocrinol Metab. 2006 Feb;290(2):E355-62. doi: 10.1152/ajpendo.00259.2005. Epub 2005 Oct 11.

  PMID: 16219668 RESULT

• Thackray AE, Barrett LA, Tolfrey K. High-Intensity Running and Energy Restriction Reduce Postprandial Lipemia in Girls. Med Sci Sports Exerc. 2016 Mar;48(3):402-11. doi: 10.1249/MSS.0000000000000788.

  PMID: 26460628 RESULT

• Wagmacker DS, Petto J, Fraga AS, Matias JB, Mota SKA, Rodrigues LEA, Ladeia AM. Metabolic Reponses to a physical exercise session in women with excess body mass: randomized clinical trial. Lipids Health Dis. 2017 Dec 19;16(1):249. doi: 10.1186/s12944-017-0600-9.

  PMID: 29258520 DERIVED

MeSH Terms

Conditions

Obesity; Motor Activity

Interventions

Exercise

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Behavior

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Ana Marice Prof Teixeira Ladeia, Doctor

  Bahian School of Medicine and Public Health

  STUDY DIRECTOR

Central Study Contacts

Ana Marice Prof Teixeira Ladeia, Doctor

CONTACT

(55) 71 99964 2420; analadeia@uol.com.br

Djeyne Prof Silveira wagmacker, MsC

CONTACT

(55) 75 99216 8222; djeyne@hotmail.com

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Will be included 66 women, randomly divided into two groups, control and experiment, overweight, sedentary and aged 18-30 years. After a 12-hour fast, baseline blood collection will be performed in both groups. The experiment group, 12 hours after the first collection, will be submitted to a physical exercise session with energy expenditure of 250Kcal. The volunteers in the control and experiment group will make a second blood collection 24 hours after the first one.

Study Record Dates

• First Submitted: May 25, 2017
• First Posted: May 31, 2017
• Study Start: May 1, 2017
• Primary Completion: July 1, 2017
• Study Completion: September 1, 2017
• Last Updated: May 31, 2017

Record last verified: 2017-04

Data Sharing

• IPD Sharing: Will not share

Locations

BR (1)