NCT01808820

Brief Summary

The purpose of this research study is to evaluate an investigational vaccine using patent-derived dendritic cells (DC) to treat malignant glioma or glioblastoma.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
20

participants targeted

Target at P25-P50 for phase_1

Timeline
Completed

Started Aug 2013

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 6, 2013

Completed
5 days until next milestone

First Posted

Study publicly available on registry

March 11, 2013

Completed
5 months until next milestone

Study Start

First participant enrolled

August 21, 2013

Completed
5.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 7, 2018

Completed
3.7 years until next milestone

Study Completion

Last participant's last visit for all outcomes

July 16, 2022

Completed
Last Updated

July 20, 2022

Status Verified

July 1, 2022

Enrollment Period

5.2 years

First QC Date

March 6, 2013

Last Update Submit

July 19, 2022

Conditions

Keywords

HGGDendritic Cell VaccineDC VaccineLeukapheresis

Outcome Measures

Primary Outcomes (1)

  • Number of Participants with Treatment-Related Adverse Events

    Adverse Events will be evaluated using the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI-CTCAE) Version 4.0 by treating physician

    Up to Week 32 (30 days after last dose of protocol therapy)

Secondary Outcomes (6)

  • Rate of Overall Survival (OS) in Study Participants

    Up to Week 80 (5 years post therapy)

  • Rate of Progression-Free Survival (PFS) in Study Participants

    Up to Week 80 (5 years post therapy)

  • Change in MDSC Levels

    Baseline, Up to Week 28

  • Change in blood counts

    Baseline, Up to Week 28

  • Comparison of clinical parameters associated with outcomes in study participants to patients on other DC/Imiquimod studies.

    Up to 5 years Post-Therapy

  • +1 more secondary outcomes

Study Arms (2)

Safety Pilot: DC Vaccine/Lysate

EXPERIMENTAL

Participants in this group will undergo leukapheresis after standard of care surgical tumor resection. Leukapheresis will be used to obtain peripheral blood mononuclear cells (PBMC) from which the dendritic cells (DC) will be obtained. Retrieved DC will be used as a vaccine once weekly on Weeks 1-4 within 3 weeks from end of leukapheresis. Participants will also receive Imiquimod, which will be applied one evening prior to DC dose for 8 hours then for 8 hours each of the next two evenings. Enrollment of participants in the Pilot group will be staggered until the second participant has no treatment limiting toxicities. For the first five subjects to be enrolled in the pilot, the administration of DC to each subject will be delayed until the prior subject has received the second administration of DC. Participants will also receive Lysate of tumor administered every 4 weeks + 3 days on Weeks 8, 12, 16 and 28 (+ / - 3 days).

Biological: Dendritic Cell VaccineBiological: Tumor LysateDrug: ImiquimodProcedure: Leukapheresis

Expansion Cohort: DC Vaccine/Lysate

EXPERIMENTAL

Participants in this group will undergo leukapheresis within after standard of care surgical tumor resection. Leukapheresis will be used to obtain peripheral blood mononuclear cells (PBMC) from which the dendritic cells (DC) will be obtained. Retrieved DC will be used as a vaccine once weekly on Weeks 1-4 within 3 weeks from end of pheresis. Participants will also receive Imiquimod, which will be applied one evening prior to DC dose for 8 hours then for 8 hours each of the next two evenings. Participants will also receive Lysate of tumor administered every 4 weeks + 3 days on Weeks 8, 12, 16 and 28 (+ / - 3 days).

Biological: Dendritic Cell VaccineBiological: Tumor LysateDrug: ImiquimodProcedure: Leukapheresis

Interventions

Between 1.2 to 12 million DC per dose administered once a week via intradermal injection for 4 weeks.

Also known as: DC Vaccine
Expansion Cohort: DC Vaccine/LysateSafety Pilot: DC Vaccine/Lysate
Tumor LysateBIOLOGICAL

Post-DC Vaccine therapy. Up to 1.5 mg of Lysate of tumor per dose administered via intradermal injection at intervals defined by study protocol.

Also known as: Lysate of Tumor, Lysate Boost
Expansion Cohort: DC Vaccine/LysateSafety Pilot: DC Vaccine/Lysate

5% topical cream applied to vaccine site before and after administrations of DC vaccine or lysate

Also known as: Aldara
Expansion Cohort: DC Vaccine/LysateSafety Pilot: DC Vaccine/Lysate
LeukapheresisPROCEDURE

Baseline, post-surgery blood draw via catheter to obtain peripheral blood mononuclear cells (PBMCs) from which Dendritic cells will be obtained.

Also known as: Pheresis
Expansion Cohort: DC Vaccine/LysateSafety Pilot: DC Vaccine/Lysate

Eligibility Criteria

Age13 Years - 99 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Age: ≥ 13 years and ≤ 99 years.
  • (2a) Relapse of high grade glioma (anaplastic astrocytoma World Health Organization (WHO) grade III or glioblastoma multiforme WHO grade IV), histologically proven at first stage of disease (radiological evidence for recurrence suffices); OR (2b) Relapse of glioma, which was grade II at initial diagnosis, but which is grade III or IV at relapse based on radiological or pathological criteria.
  • Total or subtotal resection of tumor mass, confirmed by assessment by the neurosurgeon and by postoperative MRI scan within 72 hours after surgery. The post-operative assessment should demonstrate residual tumor less than or equal to 2 cm\^3 as judged by surgeon and on MRI the tumor should only show linear contrast enhancement at the border of the resection cavity or nodule less than 2 cm\^3.
  • No radiotherapy and/or chemotherapy received for at least 1 month before first DC vaccination is to be administered
  • No treatment with corticosteroids or salicylates for at least 1 week before first vaccination. Corticosteroid therapy should be rapidly weaned within 1-2 weeks after surgery.
  • Life expectancy \> 3 months.
  • Written consent by patient or parent(s) (if patient is \< 18 years) on an institutional review board (IRB)-approved informed consent form prior to any study-specific evaluation. Assent is required from children as per University of Miami (UM) IRB guidelines. Subject must be capable of understanding the investigational nature, potential risks and benefits of the study and able to provide valid informed consent.
  • Adequate organ function (to be measured at enrollment)
  • Absolute neutrophil count (ANC) ≥ 0.75 10\*3/µl
  • Lymphocytes ≥ 0.5 10\*3/µl
  • Platelets ≥ 75 10\*3/µl
  • Hemoglobin ≥ 9 g/dL
  • Aspartate transaminase (AST)/Alanine transaminase (ALT) ≤ 2.5 X upper limit of normal (ULN); if liver metastases, ≤ 5 X ULN
  • Serum Creatinine ≤ 1.5 X ULN
  • Total Bilirubin ≤ 3 X ULN
  • +3 more criteria

You may not qualify if:

  • Pregnancy.
  • Breast feeding females.
  • Any concomitant participation in other therapeutic trials.
  • Virus serology positive for HIV (testing is not required in the absence of clinical suspicion).
  • Documented immunodeficiency or autoimmune disease.
  • Mandatory treatment with corticosteroids or salicylates in the week prior to first vaccination.
  • Other active malignancies.
  • Patients with unresectable tumors, for instance pontine gliomas, are excluded.
  • Refusal to use adequate contraception for fertile patients (females and males) during the study and for 30 days after the last dose of study treatment.
  • Any serious or uncontrolled medical or psychiatric condition that in the opinion of the investigator makes the patient not able to participate in the study.
  • Application of gliadel wafers within the prior 4 months or a plan to place gliadel wafers at the time of resection for tumor acquisition for study.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Miami

Miami, Florida, 33136, United States

Location

MeSH Terms

Conditions

GliomaGlioblastomaAstrocytoma

Interventions

lentiviral minigene vaccine of COVID-19 coronavirusImiquimodLeukapheresisBlood Component Removal

Condition Hierarchy (Ancestors)

Neoplasms, NeuroepithelialNeuroectodermal TumorsNeoplasms, Germ Cell and EmbryonalNeoplasms by Histologic TypeNeoplasmsNeoplasms, Glandular and EpithelialNeoplasms, Nerve Tissue

Intervention Hierarchy (Ancestors)

AminoquinolinesQuinolinesHeterocyclic Compounds, 2-RingHeterocyclic Compounds, Fused-RingHeterocyclic CompoundsCytapheresisBiological TherapyTherapeuticsLeukocyte Reduction ProceduresCell SeparationCytological TechniquesClinical Laboratory TechniquesInvestigative Techniques

Study Officials

  • Macarena De La Fuente, MD

    University of Miami

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Assistant Professor of Clinical

Study Record Dates

First Submitted

March 6, 2013

First Posted

March 11, 2013

Study Start

August 21, 2013

Primary Completion

November 7, 2018

Study Completion

July 16, 2022

Last Updated

July 20, 2022

Record last verified: 2022-07

Data Sharing

IPD Sharing
Will not share

Locations