Examination of Certain Blood Parameters Following Performance and Acute Exercise at Specific Times of the Day

NCT07825740 | Completed

• 2 other identifiers: 2025-259282025
• Study Type: interventional
• Target: 8
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to investigate whether the time of day at which acute exercise is performed influences physical performance and acute hormonal and biochemical responses in healthy young male soccer players. Participants will complete the same standardized exercise protocol in the morning (08:00-09:30) and evening (17:00-18:30), with a 96-hour interval between sessions. Performance will be assessed using vertical jump and Yo-Yo shuttle run tests. Blood samples will be collected before exercise, immediately after exercise, and two hours after exercise to assess testosterone, cortisol, creatine kinase, AST, ALT, LDH, and troponin levels. The study will examine whether exercise responses differ between morning and evening sessions and whether these differences are consistent with circadian variation.

Conditions

Exercise; Physical Performance; Circadian Rhythm

Keywords

Exercise1, Cortisol2, Creatine Kinase3, Circadian Rhythm4, Testosterone5,

Outcome Measures

Primary Outcomes (1)

• Yo-Yo Shuttle Run Performance

  Change in endurance performance measured by the Yo-Yo shuttle run test following the standardized acute exercise protocol performed in the morning and evening.

  Immediately after completion of the exercise performance assessment during each experimental session.

Secondary Outcomes (8)

• Vertical Jump Performance

  Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.

• Serum Testosterone Concentration

  Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.

• Serum Cortisol Concentration

  Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.

• Serum Creatine Kinase (CK) Concentration

  Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.

• Serum Aspartate Aminotransferase (AST) Concentration

  Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.

Study Arms (1)

Healthy Male Soccer Players

EXPERIMENTAL

Healthy male soccer players participated in a single-group intervention study. All participants completed the same standardized acute exercise protocol twice, once in the morning (08:00-09:30) and once in the evening (17:00-18:30), separated by 96 hours. Performance tests (vertical jump and Yo-Yo shuttle run) and blood samples were obtained before exercise, immediately after exercise, and 2 hours after exercise to evaluate hormonal and biochemical responses.

Behavioral: Standardized Acute Exercise Protocol

Interventions

Standardized Acute Exercise Protocol BEHAVIORAL

Participants completed a standardized acute exercise protocol consisting of a vertical jump test and a Yo-Yo shuttle run endurance test. The protocol was performed twice, once in the morning (08:00-09:30) and once in the evening (17:00-18:30), with a 96-hour washout period between sessions. Blood samples were collected before exercise, immediately after exercise, and 2 hours after exercise to assess hormonal (testosterone and cortisol) and biochemical (CK, AST, ALT, LDH, and troponin) responses.

Healthy Male Soccer Players

Eligibility Criteria

• Age: 18 Years - 25 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

* Healthy male soccer players. * Age between 18 and 25 years. * Regular participation in organized soccer training. * Free from musculoskeletal injury during the previous 6 months. * Able and willing to provide written informed consent.

Contact the study team to discuss eligibility requirements. They can help determine if this study is right for you.

Sponsors & Collaborators

• Firat University: lead

Study Sites (1)

MUNZUR University

Tunceli, 62100, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Masking Details: This is an open-label, single-group repeated-measures study. No masking or blinding procedures are used. Participants, investigators, and outcome assessors are aware of the exercise session and the time of day at which each assessment is performed.
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: ASSOCIATE PROFESSOR

Study Record Dates

• First Submitted: July 8, 2026
• First Posted: September 17, 2026
• Study Start: November 25, 2025
• Primary Completion: December 15, 2025
• Study Completion: January 15, 2026
• Last Updated: September 17, 2026

Record last verified: 2026-09

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)