A Single-Arm Pre-Post Study

NCT07765290 | Completed

• 1 other identifier: KBU-HIIT-2023-01
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

This interventional single-arm pre-post study aimed to investigate the acute effects of repeated Wingate exercise on free thyroid hormone responses in male amateur soccer players. Twenty healthy male amateur soccer players aged 19-22 years completed a standardized repeated Wingate exercise protocol. Blood samples were collected immediately before and after exercise to determine serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. The study evaluated the immediate endocrine responses to repeated high-intensity anaerobic exercise and their potential implications for exercise physiology and thyroid hormone regulation.

Conditions

Exercise Physiology

Keywords

free triiodothyronine; free thyroxine; thyroid-stimulating hormone; high-intensity interval exercise; Wingate anaerobic test; soccer; acute endocrine response

Outcome Measures

Primary Outcomes (1)

• Change in Serum Free Thyroid Hormone Concentrations (fT3, fT4, and TSH)

  The primary outcome was the change in serum free thyroid hormone concentrations, including free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH), measured immediately before and immediately after the repeated Wingate exercise protocol. Hormone concentrations were determined from venous blood samples to evaluate the acute endocrine response to repeated high-intensity anaerobic exercise.

  Immediately before and immediately after the repeated Wingate exercise session

Study Arms (1)

Repeated Wingate Exercise

EXPERIMENTAL

Participants completed a standardized repeated Wingate exercise protocol under laboratory conditions. Blood samples were collected immediately before and immediately after the exercise session to assess acute changes in serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH).

Behavioral: Repeated Wingate Exercise

Interventions

Repeated Wingate Exercise BEHAVIORAL

Participants completed a standardized repeated Wingate exercise protocol under controlled laboratory conditions to investigate the acute effects of high-intensity anaerobic exercise on thyroid hormone responses. Venous blood samples were collected immediately before and immediately after the exercise session to measure serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations.

Also known as: Repeated Wingate Test

Repeated Wingate Exercise

Eligibility Criteria

• Age: 19 Years - 22 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age between 19 and 22 years.
• Apparently healthy and free from acute or chronic disease.
• Regular participation in amateur soccer training.
• Willingness to participate and provision of written informed consent.

You may not qualify if:

• Current use of medications or supplements known to affect thyroid function or hormonal responses.
• Acute illness or musculoskeletal injury at the time of testing.
• Failure to complete the repeated Wingate exercise protocol or all required blood sample collections.

Sponsors & Collaborators

• Inonu University: lead
• İnönü University: collaborator

Study Sites (1)

Exercise Physiology Laboratory, Faculty of Sport Sciences, Karabuk University

Karabük, Karabük Province, 78050, Turkey (Türkiye)

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Masking Details: No masking was used in this study. All participants received the same repeated Wingate exercise intervention, and outcome measurements were performed before and immediately after the intervention using a single-group pre-post study design. Neither participants nor investigators were blinded because the exercise intervention and blood sample collection required direct participant involvement.
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assoc. Prof.Dr.

Model Details: This study used a single-group pre-post design in which all participants received the same repeated Wingate exercise intervention. Serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations were measured immediately before and immediately after the exercise protocol to evaluate the acute effects of repeated high-intensity anaerobic exercise on thyroid hormone responses.

Study Record Dates

• First Submitted: August 11, 2026
• First Posted: August 14, 2026
• Study Start: June 15, 2023
• Primary Completion: June 30, 2023
• Study Completion: June 30, 2023
• Last Updated: August 14, 2026

Record last verified: 2026-08

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)