NCT07765290

Brief Summary

This two-group, non-randomized study investigated the acute effects of repeated Wingate exercise on free thyroid hormone concentrations in male amateur soccer players. Twenty healthy male amateur soccer players completed a standardized repeated Wingate exercise protocol, and a separate group of twenty male amateur soccer players provided two morning blood samples without exercising. Blood samples were collected immediately before and immediately after the exercise session or the resting interval to determine free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. Including a non-exercising comparison group allows the observed changes to be evaluated against the change expected across a comparable interval in the absence of exercise.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
40

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Jun 2023

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 15, 2023

Completed
15 days until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 30, 2023

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 30, 2023

Completed
3.1 years until next milestone

First Submitted

Initial submission to the registry

August 11, 2026

Completed
3 days until next milestone

First Posted

Study publicly available on registry

August 14, 2026

Completed
Last Updated

September 23, 2026

Status Verified

September 1, 2026

Enrollment Period

15 days

First QC Date

August 11, 2026

Last Update Submit

September 18, 2026

Conditions

ExerciseThyroid Function

Keywords

free triiodothyroninefree thyroxinethyroid-stimulating hormonehigh-intensity interval exerciseWingate anaerobic testsocceracute endocrine response

Outcome Measures

Primary Outcomes (1)

  • Change in Serum Free Thyroid Hormone Concentrations (fT3, fT4, and TSH)

    The primary outcome was the within-participant change in serum free thyroid hormone concentrations, including free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH), measured immediately before and immediately after the repeated Wingate exercise protocol in the exercise group and across a comparable resting interval in the comparison group. Change scores were compared between groups.

    Immediately before and immediately after the repeated Wingate exercise session or the resting interval

Study Arms (2)

Repeated Wingate Exercise

EXPERIMENTAL

Participants completed a standardized repeated Wingate exercise protocol under laboratory conditions. Blood samples were collected immediately before and immediately after the exercise session to assess acute changes in serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH).

Behavioral: Repeated Wingate Exercise

Comparison group

NO INTERVENTION

Participants provided two venous blood samples on one morning and performed no exercise between the two samples. Serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) were measured in both samples.

Interventions

Repeated Wingate ExerciseBEHAVIORAL

Participants completed a standardized repeated Wingate exercise protocol under controlled laboratory conditions to investigate the acute effects of high-intensity anaerobic exercise on thyroid hormone responses. Venous blood samples were collected immediately before and immediately after the exercise session to measure serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations.

Also known as: Repeated Wingate Test
Repeated Wingate Exercise

Eligibility Criteria

Age18 Years - 25 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Male amateur soccer players.
  • Age between 18 and 25 years.
  • At least 6 years of licensed soccer experience.
  • Actively participating in amateur soccer training.
  • Apparently healthy and free from acute or chronic disease on medical history review.
  • Willingness to participate and provision of written informed consent.

You may not qualify if:

  • Acute illness or musculoskeletal injury at the time of testing.
  • Neurological, auditory, visual or vestibular disorder.
  • Reduced physical capacity due to injury, or a sedentary lifestyle.
  • History of nutritional or pharmacological ergogenic aid use.
  • Failure to complete the study procedures or all required blood sample collections.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Exercise Physiology Laboratory, Faculty of Sport Sciences, Karabuk University

Karabük, Karabük Province, 78050, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

  • Özgür EKEN, PhD

    Inonu University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Masking Details
No masking was used in this study. Participants were aware of their group assignment because the exercise protocol and blood sample collection required direct participant involvement. Laboratory personnel were not blinded to group or to sampling time point.
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Model Details: This study used a two-group, non-randomized, non-concurrent design. One group completed a repeated Wingate exercise protocol; a separate comparison group provided two morning blood samples without exercising. Serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations were measured immediately before and immediately after the exercise protocol or the resting interval. The two groups were recruited and sampled in different periods rather than concurrently, so participants were not randomly allocated to conditions.
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assoc. Prof.Dr.

Study Record Dates

First Submitted

August 11, 2026

First Posted

August 14, 2026

Study Start

June 15, 2023

Primary Completion

June 30, 2023

Study Completion

June 30, 2023

Last Updated

September 23, 2026

Record last verified: 2026-09

Data Sharing

IPD Sharing
Will share

De-identified participant-level data and analysis code are available from the corresponding author on reasonable request, subject to applicable ethics, participant-consent, and institutional requirements.

Shared Documents
ANALYTIC CODE
Time Frame
Data will be available from the date of publication, with no planned end date.
Access Criteria
Requests should be directed to the corresponding author. Data will be shared with researchers who provide a methodologically sound proposal, subject to applicable ethics approval, participant-consent restrictions, and institutional requirements. A data-use agreement may be required.

Locations

TU(1)