NCT07644000

Brief Summary

The goal of this clinical trial is to evaluate if molecular hydrogen can reduce the metabolic response during graded exercise in healthy women aged 18-30 years. The main questions it aims to answer are:

  • Does molecular hydrogen reduce the respiratory exchange ratio?
  • Does molecular hydrogen reduce oxygen uptake? Researchers will compare molecular hydrogen to a placebo (ambient air containing no molecular hydrogen) to see if molecular hydrogen reduces the metabolic response. Participants will:
  • Inhale molecular hydrogen for 60 minutes each day for 7 days.
  • Undergo an exercise testing twice after a two-week washout period.

Trial Health

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Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
22

participants targeted

Target at below P25 for not_applicable

Timeline
5mo left

Started Jul 2026

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 8, 2026

Completed
4 days until next milestone

First Posted

Study publicly available on registry

June 12, 2026

Completed
19 days until next milestone

Study Start

First participant enrolled

July 1, 2026

Expected
5 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2026

Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2026

Last Updated

June 12, 2026

Status Verified

June 1, 2026

Enrollment Period

5 months

First QC Date

June 8, 2026

Last Update Submit

June 8, 2026

Conditions

Healthy Adult Females

Keywords

molecular hydrogenmetabolic response

Outcome Measures

Primary Outcomes (1)

  • Respiratory exchange ratio

    Respiratory exchange ratio is measured using an Ergostik analyser (Geratherm Respiratory, Bad Kissinger, Germany) breath by breath during the exercise, and the average is calculated for 6-minute segments for subsequent analysis.

    Change after 2 weeks of crossover.

Secondary Outcomes (7)

  • Oxygen uptake

    Change after 2 weeks of crossover.

  • Carbon dioxide production

    Change after 2 weeks of crossover.

  • Minute ventilation

    Change after 2 weeks of crossover.

  • Breathing frequency

    Change after 2 weeks of crossover.

  • Heart rate

    Change after 2 weeks of crossover.

  • +2 more secondary outcomes

Other Outcomes (1)

  • Blood glucose concentration

    Change after 2 weeks of crossover.

Study Arms (2)

Molecular hydrogen

EXPERIMENTAL

Molecular hydrogen inhalation for 60 minutes daily for 7 days.

Other: Molecular hydrogen

Placebo

PLACEBO COMPARATOR

Ambient air inhalation for 60 minutes daily for 7 days.

Other: Placebo

Interventions

Molecular hydrogenOTHER

Gas mixture containing 3.75% molecular hydrogen and ambient air.

Also known as: H2 Air-Blend Generator (H2 Medical Technologies, Ostrava, Czech Republic)
Molecular hydrogen
PlaceboOTHER

Ambient air without molecular hydrogen.

Placebo

Eligibility Criteria

Age18 Years - 30 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility DetailsCisgender women.
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy female participants.
  • Regular menstrual cycle with exercise testing scheduled outside the menstrual phase.
  • Signed informed consent.

You may not qualify if:

  • Pregnancy or breastfeeding.
  • History or presence of cardiovascular disease.
  • History or presence of respiratory disease.
  • Regular use of medications affecting metabolism.
  • Use of dietary supplements affecting metabolism within 14 days prior to intervention.
  • Strenuous physical activity within 24 hours prior to exercise testing.
  • Any contraindication to exercise testing or study participation determined by a physician.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Palacky University, Faculty of Physical Culture

Olomouc, 77111, Czechia

Location

MeSH Terms

Interventions

Hydrogen

Intervention Hierarchy (Ancestors)

ElementsInorganic ChemicalsGases

Study Officials

  • Tereza Rysava

    Palacky University

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Tereza Rysava

CONTACT

+420585636154tereza.rysava01@upol.cz

Jakub Krejci, PhD

CONTACT

jakub.krejci@upol.cz

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant professor

Study Record Dates

First Submitted

June 8, 2026

First Posted

June 12, 2026

Study Start (Estimated)

July 1, 2026

Primary Completion (Estimated)

December 1, 2026

Study Completion (Estimated)

December 1, 2026

Last Updated

June 12, 2026

Record last verified: 2026-06

Data Sharing

IPD Sharing
Will share

All IPD that underlie results in a publication.

Time Frame
Beginning 1 month after the publication of results with no end date.
Access Criteria
Anonymized IPD will be uploaded to an open-access data repository.

Locations

CZ(1)