Amino Acid Oxidation of Whole-Food Protein: a Dose-Response Crossover Trial in Young Adults

NCT07547176 | Recruiting DMC

• 2 other identifiers: METC 25-011NL-009478
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to assess the amino acid oxidation dose-response relationship of ingested whole-food protein in healthy adults. In a randomized, crossover design, 20 young, healthy adult males and females aged 18-35 years, will consume a dinner consisting of 0.32, 0.74, and 1.32 g protein･kg BM-1 (corresponding to 1, 2, and 4 minced beef patties, respectively) during 3 separate experimental trials.The main study endpoint is the amino acid oxidation of ingested protein over a 24-h period.

Conditions

Protein Metabolism

Outcome Measures

Primary Outcomes (1)

• Amino acid oxidation of ingested protein

  Amino acid oxidation; compared between interventions to determine the dose-response relationship of amino acid oxidation to ingested whole-food protein.

  Over the 24 hour timeframe following protein ingestion

Secondary Outcomes (1)

• Plasma amino acid response of ingested protein

  Over the 24 hour timeframe following protein ingestion

Study Arms (3)

Dinner consisting of 0.32 g protein･kg BM-1

EXPERIMENTAL

Dietary Supplement: Food proteins

Dinner consisting of 0.74 g protein･kg BM-1

EXPERIMENTAL

Dietary Supplement: Food proteins

Dinner consisting of 1.32 g protein･kg BM-1

EXPERIMENTAL

Dietary Supplement: Food proteins

Interventions

Food proteins DIETARY_SUPPLEMENT

Dinner meal consisting of minced beef protein.

Dinner consisting of 0.32 g protein･kg BM-1; Dinner consisting of 0.74 g protein･kg BM-1; Dinner consisting of 1.32 g protein･kg BM-1

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Aged between 18-35 years
• BMI between 18.5-30 kg/m2
• Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)
• No physical limitations (i.e., able to perform all activities associated with daily living independently)

You may not qualify if:

• Smoking
• Vegan, vegetarian, or does not consume beef protein
• Diagnosed musculoskeletal disorders
• Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
• Chronic use of gastric acid-suppressing medication or anti-coagulants
• Unstable weight over the last three months
• Diagnosed GI tract disorders or diseases
• Blood donation in the past 2 months
• Females: pregnancy

Sponsors & Collaborators

• Maastricht University Medical Center: lead

Study Sites (1)

Maastricht University Medical Centre+

Maastricht, Limburg, 6200 MD, Netherlands

RECRUITING

Study Officials

• Luc JC van Loon, PhD

  Maastricht University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Julia M Malowany, MSc

CONTACT

+31 43 388 1617; julia.malowany@maastrichtuniversity.nl

Jorn Trommelen, PhD

CONTACT

+31 43 388 1617; jorn.trommelen@maastrichtuniversity.nl

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Within-subject, randomized, crossover design

Study Record Dates

• First Submitted: March 25, 2026
• First Posted: April 23, 2026
• Study Start: November 1, 2025
• Primary Completion (Estimated): March 1, 2027
• Study Completion (Estimated): March 1, 2027
• Last Updated: April 23, 2026

Record last verified: 2026-04

Locations

NL (1)