Digestion and Absorption Kinetics of Beef Consumed Alone or as Part of a Mixed-Meal: a Crossover Trial in Young Adults

NCT07552740 | Not Yet Recruiting DMC

• 2 other identifiers: METC 25-032NL-010299
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to assess the digestion and absorption kinetics of beef consumed alone or as part in healthy adults. In a randomized, crossover design, 10 young, healthy adult males and females aged 18-35 years, will consume a meal consisting of 25 g protein from minced beef alone or as part of a mixed-meal during two separate experimental trials. The main study endpoint is the exogenous rate of amino acid appearance in response to the ingested beef over a 10-h period.

Conditions

Protein Metabolism

Outcome Measures

Primary Outcomes (1)

• Digestion and absorption kinetics

  The exogenous rate of amino acid appearance in response to the ingested beef (expressed as μmol/kg/min). Based on tracer plasma amino acid concentrations over time (measured with ultra performance liquid chromatography-mass spectrometry).

  Over the 10 hour timeframe following beef ingestion

Secondary Outcomes (1)

• Whole-body protein metabolism

  Over the 10 hour timeframe following beef ingestion

Study Arms (2)

Beef only

EXPERIMENTAL

Dietary Supplement: Food proteins

Beef in mixed-meal

EXPERIMENTAL

Dietary Supplement: Food proteins

Interventions

Food proteins DIETARY_SUPPLEMENT

Meal consisting of 25 g protein･kg BM-1 from minced beef protein.

Beef in mixed-meal; Beef only

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Aged between 18-35 years
• BMI between 18.5-30 kg/m2
• Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)
• No physical limitations (i.e., able to perform all activities associated with daily living independently)

You may not qualify if:

• Smoking
• Vegan, vegetarian, or does not consume beef protein
• Diagnosed musculoskeletal disorders
• Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
• Chronic use of gastric acid-suppressing medication or anti-coagulants
• Unstable weight over the last three months
• Diagnosed GI tract disorders or diseases
• Blood donation in the past 2 months
• Females: pregnancy

Sponsors & Collaborators

• Maastricht University Medical Center: lead

Study Sites (1)

Maastricht University Medical Centre+

Maastricht, Limburg, 6200 MD, Netherlands

Location

Study Officials

• Luc JC van Loon, PhD

  Maastricht University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Julia M Malowany, MSc

CONTACT

+31 43 388 1617; julia.malowany@maastrichtuniversity.nl

Jorn Trommelen, PhD

CONTACT

+31 43 388 1617; jorn.trommelen@maastrichtuniversity.nl

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Within-subject, randomized, crossover design

Study Record Dates

• First Submitted: January 15, 2026
• First Posted: April 27, 2026
• Study Start (Estimated): September 1, 2026
• Primary Completion (Estimated): September 1, 2027
• Study Completion (Estimated): January 1, 2028
• Last Updated: April 27, 2026

Record last verified: 2026-04

Locations

NL (1)