Animal and Microbial-based Dietary Protein Efficiency in Adults

NCT07148908 | Recruiting

• 1 other identifier: REB00048239
• Study Type: interventional
• Target: 13
• Locations: 1 country
• Sites: 1

Brief Summary

Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.

Conditions

Protein Metabolism

Keywords

Protein quality; protein metabolism; stable isotopes; protein oxidation; indicator amino acid oxidation; protein synthesis

Outcome Measures

Primary Outcomes (2)

• 1. Phenylalanine excretion (umol/kg/h)

  Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).

  8 hours

• Phenylalanine oxidation (umol/kg/h)

  Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period.

  8 hours

Study Arms (3)

Yeast Protein Supplement

EXPERIMENTAL

Participants will be consuming test beverage composed of yeast protein and their whole-body protein metabolism will be determined over the subsequent 8 hours.

Dietary Supplement: Yeast Protein Supplement

Whey Protein Isolate Supplement

ACTIVE COMPARATOR

Participants will be consuming test beverage composed of whey protein isolate and their whole-body protein metabolism will be determined over the subsequent 8 hours.

Dietary Supplement: Whey Protein Isolate Supplement

Collagen Hydrolysate Supplement

ACTIVE COMPARATOR

Participants will be consuming test beverage composed of collagen protein and their whole-body protein metabolism will be determined over the subsequent 8 hours.

Dietary Supplement: Collagen Hydrolysate Supplement

Interventions

Yeast Protein Supplement DIETARY_SUPPLEMENT

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Yeast Protein Supplement

Whey Protein Isolate Supplement DIETARY_SUPPLEMENT

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Whey Protein Isolate Supplement

Collagen Hydrolysate Supplement DIETARY_SUPPLEMENT

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Collagen Hydrolysate Supplement

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy, male and female, recreationally-active participants.
• Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C).
• Participants will be aged 18-45 years old.
• Participants are willing to abide by the compliance rules of this study.
• Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
• Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants).

You may not qualify if:

• Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy).
• Self-reported regular tobacco use.
• Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.).
• Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
• Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants).
• Use of COCs containing \>21 active pills per cycle due to potential differences in their effect on metabolism (female participants).
• Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants).
• Not currently or previously (in the past 6 months) on a vegan diet

Sponsors & Collaborators

• Daniel Moore: lead
• Lesaffre International: collaborator

Study Sites (1)

Kinesiology & Physical Education

Toronto, Ontario, M5S 2C9, Canada

RECRUITING

Central Study Contacts

Daniel R. Moore, Ph.D.

CONTACT

416-946-4088; drmoore@utoronto.ca

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Principal Investigator

Model Details: Single-blind randomized, counterbalanced design Thirteen healthy young (18-45 years of age, at least 6 females and 6 males) will be subjected to a non-invasive 13CO2 breath-test based on the indicator amino acid oxidation method and urine collections following hourly ingestion of protein test beverages (whey, yeast, and collagen randomized by trials).

Study Record Dates

• First Submitted: August 22, 2025
• First Posted: August 29, 2025
• Study Start: July 1, 2025
• Primary Completion: October 31, 2025
• Study Completion: December 31, 2025
• Last Updated: August 29, 2025

Record last verified: 2025-08

Locations

CA (1)