The Effect of a Single Training Session on Changes in Inflammatory, Hormonal, and Metabolic Markers in Athletes

NCT07264751 | Completed

• 1 other identifier: AKS wrestlers
• Study Type: observational
• Target: 5
• Locations: 1 country
• Sites: 1

Brief Summary

The study aims to assess the acute response of wrestlers to a single training session. Short-term changes in inflammatory, hormonal, and metabolic markers are analyzed to assess fatigue development and recovery following intense exercise. Participation in the study involves completing a standard training session and providing blood samples at three time points to assess the physiological response. The results provide a better understanding of the mechanisms underlying adaptation to exercise in competitive wrestlers.

Conditions

Training; Hormone; Exercise Training; Inflammation Biomarkers

Keywords

Single training session; Inflammatory markers; Hormonal response; Wrestlers; Catecholamines

Outcome Measures

Primary Outcomes (6)

• Concentration of high-sensitivity C-reactive protein (hsCRP)

  High-sensitivity C-reactive protein (hsCRP) concentration will be measured to assess the inflammatory response.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of creatine kinase (CK)

  Creatine kinase (CK) concentration will be measured as a biomarker of muscle damage and the physiological response to the training session.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of tumor necrosis factor-alpha (TNF-α).

  Tumor necrosis factor-alpha (TNF-α) concentration will be assessed as a marker of the inflammatory response to training.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of interleukin-6 (IL-6).

  Interleukin-6 (IL-6) concentration will be assessed as a marker of the inflammatory response to training.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of adrenaline

  Adrenaline concentration will be assessed as a marker of the acute sympathoadrenal response to training.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of noradrenaline

  Noradrenaline concentration will be assessed as a marker of the acute sympathoadrenal response to training.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

Secondary Outcomes (4)

• Concentration of cortisol

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of total protein.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of urea.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

• Concentration of albumin.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

Other Outcomes (1)

• Concentration of uric acid.

  1. Before training (at rest), 2. Immediately after training, 3. 24 hours after exercise.

Interventions

physical training BEHAVIORAL

The intervention consists of a standardized, high-intensity wrestling training session designed to simulate typical strength-endurance exercise performed by elite wrestlers. The session includes sport-specific drills, resistance exercises, and aerobic conditioning components appropriate for this athletic discipline. All participants will perform the session under supervision to ensure safety and adherence to the planned intensity and duration.

Eligibility Criteria

• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The study participants are athletes from a national-level sports club who were also members of the national team. All athletes have experience competing in international events. The group includes athletes who have won medals at both national and international competitions. Participants are wrestling athletes specializing in strength-endurance training and represent the top competitive level in their sport. The study aims to evaluate acute physiological responses to a single high-intensity training session in this highly specialized discipline.

You may qualify if:

• Currently engaged in regular strength-endurance wrestling training.
• Medically cleared for high-intensity exercise by a sports physician.
• Willingness to participate and provide written informed consent.
• Availability to attend the training session and all blood sampling time points (pre-training, immediately post-training, 24 hours post-training).

You may not qualify if:

• Presence of acute or chronic illness that may affect exercise performance or physiological response.
• Recent musculoskeletal injury limiting participation in high-intensity training.
• Use of medications or supplements that may interfere with hormonal, inflammatory, or metabolic markers.
• History of endocrine disorders, adrenal insufficiency, or other conditions affecting catecholamine or cortisol levels.
• Inability or unwillingness to comply with study procedures.

Sponsors & Collaborators

• Poznan University of Physical Education: lead

Study Sites (1)

Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow Wielkopolski, Poland; Department of Biological Sciences.

Gorzów Wielkopolski, 66-400, Poland

Location

MeSH Terms

Interventions

Physical Conditioning, Human

Intervention Hierarchy (Ancestors)

Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Anna Kasperska, PhD

  Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow Wielkopolski, Poland;

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: November 23, 2025
• First Posted: December 4, 2025
• Study Start: February 1, 2014
• Primary Completion: May 1, 2014
• Study Completion: June 1, 2014
• Last Updated: August 13, 2026

Record last verified: 2026-08

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)