Mitochondrial Energy Metabolism in Obese Women

NCT03119350 | Completed DMC

• 1 other identifier: Process HCRP: 1.387.040/2016
• Study Type: interventional
• Target: 14
• Locations: 1 country
• Sites: 1

Brief Summary

Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses:

1. 1.The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ;
2. 2.The unbalance is therapeutically modified through physical training ;
3. 3.Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program.

Conditions

Metabolism Disorder; Mitochondrial Alteration; Physical Activity; Obesity

Outcome Measures

Primary Outcomes (13)

• Changes Body weight

  Body weight was measured by digital balance before and after the intervention

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes Body composition

  The change in body composition through deuterium oxide was evaluated.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes White adipose tissue biopsy

  A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3).

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes Indirect calorimetry

  With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates).

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes in fatty acids

  Collected in lithium heparin tubes, they were centrifuged.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes oxidative stress

  Collected in lithium heparin tubes, they were centrifuged.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes inflammatory cytokines

  Collected in lithium heparin tubes, they were centrifuged.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes in total cholesterol

  Collected in lithium heparin tubes, they were centrifuged.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes Physical Performance

  Based on the Shuttle Walking Test adaptation.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes in Determination of Lactate

  Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes Food intake

  Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes Nitrogen Balance

  Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen.

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

• Changes Telomere length

  peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell. The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR).

  Two times: (1) First day and (2) 10 weeks after adaptation and intervention

Study Arms (1)

Physical Training

OTHER

There was concurrent physical training intervention: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation and learning to exercise, 8 weeks of physical training. Frequency: 3 times per week Duration: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.

Other: Physical Training

Interventions

Physical Training OTHER

Intervention with concurrent physical training: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation to physical exercise, 8 weeks of training. Frequency: 3 times a week. Time: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.

Physical Training

Eligibility Criteria

• Age: 20 Years - 40 Years
• Sex: female
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample

You may not qualify if:

• Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.

Sponsors & Collaborators

• University of Sao Paulo: lead

Study Sites (1)

Camila Fernanda Cunha Brandão

Ribeirão Preto, São Paulo, 14.048-900, Brazil

Location

Related Publications (28)

• BROWN, L.E.; WEIR, J.P.; ASEP procedures recommendation i: accurate assessment of muscular strength and power, Journal of Exercise Physiology, v. 4, n. 3, p. 1-21, 2001.

  BACKGROUND

• Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, Macdonald MJ, McGee SL, Gibala MJ. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J Physiol. 2008 Jan 1;586(1):151-60. doi: 10.1113/jphysiol.2007.142109. Epub 2007 Nov 8.

  PMID: 17991697 BACKGROUND

• Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.

  PMID: 2440339 BACKGROUND

• Colégio Americano de Medicina Esportiva, CAME. Guia para Teste de Esforço e Prescrição de Exercício. 3º Edição, Medsi, Rio de janeiro, RJ, p.25, 1987.

  BACKGROUND

• Crump C, Sundquist J, Winkleby MA, Sundquist K. Interactive effects of obesity and physical fitness on risk of ischemic heart disease. Int J Obes (Lond). 2017 Feb;41(2):255-261. doi: 10.1038/ijo.2016.209. Epub 2016 Nov 21.

  PMID: 27867205 BACKGROUND

• Curtis JM, Grimsrud PA, Wright WS, Xu X, Foncea RE, Graham DW, Brestoff JR, Wiczer BM, Ilkayeva O, Cianflone K, Muoio DE, Arriaga EA, Bernlohr DA. Downregulation of adipose glutathione S-transferase A4 leads to increased protein carbonylation, oxidative stress, and mitochondrial dysfunction. Diabetes. 2010 May;59(5):1132-42. doi: 10.2337/db09-1105. Epub 2010 Feb 11.

  PMID: 20150287 BACKGROUND

• Daussin FN, Zoll J, Dufour SP, Ponsot E, Lonsdorfer-Wolf E, Doutreleau S, Mettauer B, Piquard F, Geny B, Richard R. Effect of interval versus continuous training on cardiorespiratory and mitochondrial functions: relationship to aerobic performance improvements in sedentary subjects. Am J Physiol Regul Integr Comp Physiol. 2008 Jul;295(1):R264-72. doi: 10.1152/ajpregu.00875.2007. Epub 2008 Apr 16.

  PMID: 18417645 BACKGROUND

• EIGENTLER, A.; DRAXL, A.; et al. Laboratory Protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondrial Physiology Network, v. 17.04, n. 3, p. 1-11, 2015.

  BACKGROUND

• Fernstrom M, Bakkman L, Loogna P, Rooyackers O, Svensson M, Jakobsson T, Brandt L, Lagerros YT. Improved Muscle Mitochondrial Capacity Following Gastric Bypass Surgery in Obese Subjects. Obes Surg. 2016 Jul;26(7):1391-7. doi: 10.1007/s11695-015-1932-z.

  PMID: 26471784 BACKGROUND

• Ferreira FC, Bertucci DR, Barbosa MR, Nunes JE, Botero JP, Rodrigues MF, Shiguemoto GE, Santoro V, Verzola AC, Nonaka RO, Verzola RM, Baldissera V, Perez SE. Circuit resistance training in women with normal weight obesity syndrome: body composition, cardiometabolic and echocardiographic parameters, and cardiovascular and skeletal muscle fitness. J Sports Med Phys Fitness. 2017 Jul-Aug;57(7-8):1033-1044. doi: 10.23736/S0022-4707.16.06391-X. Epub 2016 Jul 6.

  PMID: 27385544 BACKGROUND

• FETT, C.A.; FETT, W.C.R.; MARCHINI, J.S. Fitness Level of Overweight/Obese Women After 08 Weeks of Aerobic or Mixed Metabolism Exercises. Revista Brasileira de Cineantropometria & Desempenho Humano, v. 11, p. 261-266, 2009.

  BACKGROUND

• Foster C. Monitoring training in athletes with reference to overtraining syndrome. Med Sci Sports Exerc. 1998 Jul;30(7):1164-8. doi: 10.1097/00005768-199807000-00023.

  PMID: 9662690 BACKGROUND

• Frayn KN. Calculation of substrate oxidation rates in vivo from gaseous exchange. J Appl Physiol Respir Environ Exerc Physiol. 1983 Aug;55(2):628-34. doi: 10.1152/jappl.1983.55.2.628.

  PMID: 6618956 BACKGROUND

• Grimble GK, West MF, Acuti AB, Rees RG, Hunjan MK, Webster JD, Frost PG, Silk DB. Assessment of an automated chemiluminescence nitrogen analyzer for routine use in clinical nutrition. JPEN J Parenter Enteral Nutr. 1988 Jan-Feb;12(1):100-6. doi: 10.1177/0148607188012001100.

  PMID: 3125351 BACKGROUND

• Heilbronn LK, Gan SK, Turner N, Campbell LV, Chisholm DJ. Markers of mitochondrial biogenesis and metabolism are lower in overweight and obese insulin-resistant subjects. J Clin Endocrinol Metab. 2007 Apr;92(4):1467-73. doi: 10.1210/jc.2006-2210. Epub 2007 Jan 23.

  PMID: 17244782 BACKGROUND

• Koh EH, Park JY, Park HS, Jeon MJ, Ryu JW, Kim M, Kim SY, Kim MS, Kim SW, Park IS, Youn JH, Lee KU. Essential role of mitochondrial function in adiponectin synthesis in adipocytes. Diabetes. 2007 Dec;56(12):2973-81. doi: 10.2337/db07-0510. Epub 2007 Sep 7.

  PMID: 17827403 BACKGROUND

• Kong Z, Sun S, Liu M, Shi Q. Short-Term High-Intensity Interval Training on Body Composition and Blood Glucose in Overweight and Obese Young Women. J Diabetes Res. 2016;2016:4073618. doi: 10.1155/2016/4073618. Epub 2016 Sep 28.

  PMID: 27774458 BACKGROUND

• Kraunsoe R, Boushel R, Hansen CN, Schjerling P, Qvortrup K, Stockel M, Mikines KJ, Dela F. Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity. J Physiol. 2010 Jun 15;588(Pt 12):2023-32. doi: 10.1113/jphysiol.2009.184754. Epub 2010 Apr 26.

  PMID: 20421291 BACKGROUND

• Lepage G, Roy CC. Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J Lipid Res. 1984 Dec 1;25(12):1391-6.

  PMID: 6530596 BACKGROUND

• Medbo JI, Mamen A, Holt Olsen O, Evertsen F. Examination of four different instruments for measuring blood lactate concentration. Scand J Clin Lab Invest. 2000 Aug;60(5):367-80. doi: 10.1080/003655100750019279.

  PMID: 11003256 BACKGROUND

• Perry CG, Lally J, Holloway GP, Heigenhauser GJ, Bonen A, Spriet LL. Repeated transient mRNA bursts precede increases in transcriptional and mitochondrial proteins during training in human skeletal muscle. J Physiol. 2010 Dec 1;588(Pt 23):4795-810. doi: 10.1113/jphysiol.2010.199448. Epub 2010 Oct 4.

  PMID: 20921196 BACKGROUND

• Pfrimer K, Moriguti JC, Lima NK, Marchini JS, Ferriolli E. Bioelectrical impedance with different equations versus deuterium oxide dilution method for the inference of body composition in healthy older persons. J Nutr Health Aging. 2012 Feb;16(2):124-7. doi: 10.1007/s12603-011-0137-y.

  PMID: 22323345 BACKGROUND

• POLLOCK, M.L.; WILMORE, J.H.; FOX III, S.M. Exercícios na Saúde e na Doença - Avaliação e prescrição para prevenção e reabilitação. Rio de Janeiro: 1986.

  BACKGROUND

• Singh SJ, Morgan MD, Scott S, Walters D, Hardman AE. Development of a shuttle walking test of disability in patients with chronic airways obstruction. Thorax. 1992 Dec;47(12):1019-24. doi: 10.1136/thx.47.12.1019.

  PMID: 1494764 BACKGROUND

• Tan S, Wang J, Cao L, Guo Z, Wang Y. Positive effect of exercise training at maximal fat oxidation intensity on body composition and lipid metabolism in overweight middle-aged women. Clin Physiol Funct Imaging. 2016 May;36(3):225-30. doi: 10.1111/cpf.12217. Epub 2014 Nov 19.

  PMID: 27072372 BACKGROUND

• WEIR JB. New methods for calculating metabolic rate with special reference to protein metabolism. J Physiol. 1949 Aug;109(1-2):1-9. doi: 10.1113/jphysiol.1949.sp004363. No abstract available.

  PMID: 15394301 BACKGROUND

• Yin X, Lanza IR, Swain JM, Sarr MG, Nair KS, Jensen MD. Adipocyte mitochondrial function is reduced in human obesity independent of fat cell size. J Clin Endocrinol Metab. 2014 Feb;99(2):E209-16. doi: 10.1210/jc.2013-3042. Epub 2013 Nov 25.

  PMID: 24276464 BACKGROUND

• Brandao CFC, Nonino CB, de Carvalho FG, Nicoletti CF, Noronha NY, San Martin R, de Freitas EC, Junqueira-Franco MVM, Marchini JS. The effects of short-term combined exercise training on telomere length in obese women: a prospective, interventional study. Sports Med Open. 2020 Jan 16;6(1):5. doi: 10.1186/s40798-020-0235-7.

  PMID: 31950310 DERIVED

MeSH Terms

Conditions

Metabolic Diseases; Motor Activity; Obesity

Interventions

Physical Conditioning, Human

Condition Hierarchy (Ancestors)

Nutritional and Metabolic Diseases; Behavior; Overweight; Overnutrition; Nutrition Disorders; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: April 10, 2017
• First Posted: April 18, 2017
• Study Start: April 1, 2016
• Primary Completion: July 1, 2016
• Study Completion: September 15, 2016
• Last Updated: April 30, 2019

Record last verified: 2019-04

Data Sharing

• IPD Sharing: Will not share

Locations

BR (1)